Doyle S, Doran MM, Cunningham C, Lowry JP (2025) Real-time in vivo monitoring of cholinergic neurotransmission in the mouse brain using a microelectrochemical choline biosensor. Eur J Neurosci 62)9):e70291. doi: 10.1111/ejn.70291 PMID: 41185145
Objective: To refine an established choline oxidase (ChOx) microelectrochemical biosensor to validate its use for long-term recording in the freely moving mouse.
Summary: Systemic administration of donepezil produced a pronounced decrease in current in both the pre-frontal cortex and hippocampus, with scopolamine and amphetamine resulting in signal increases that were not observed in animals with selective saporin lesioning (mu p75-SAP) of the cholinergic basal forebrain. Furthermore, continuous biosensor recording in both regions displayed diurnal oscillations across repetitive light–dark phases. All are consistent with successful monitoring of endogenous changes in cholinergic neurotransmission.
Usage: Two 1-μL icv injections of either sterile PBS (control animals) or mu p75-SAP (IT-16) at a concentration of 0.6 μg/μL were made into the lateral ventricles usinga NanoFil syringe under the control of an infusion pump at a rate of 0.2 nL/min. Following injection, the needle tip was left in place for 8 min to minimize reflux.
Related Products: mu p75-SAP (Cat. #IT-16)