Komatsu N, Komatsu M, Ohashi R, Horii A, Hoshi K, Takato T, Abe T, Hamakubo T (2020) Saponin facilitates anti-robo1 immunotoxin cytotoxic effects on maxillary sinus squamous cell carcinoma. J Oncol 2020:9593516. doi: 10.1155/2020/9593516 PMID: 32256588
Objective: To examine whether a flashed treatment with saponin exerted a significant specific cytotoxic effect on cancer cells.
Summary: The authors have shown previously the enhanced cytotoxic effects of saporin-conjugated anti-Robo1 immunotoxin (IT-Robo1) on the HNSCC cell line HSQ-89 in combination with a photochemical internalization technique. The combination of an immunotoxin with saponin also exhibited a significant tumor-suppressive effect on mice HSQ-19 xenograft.
Usage: A saporin-conjugated anti-Robo1 antibody (IT-Robo1) and saporin-conjugated negative control antibody (IT-NC), were prepared by incubating 121μl of 1.1μM streptavidin-saporin and 138μl of 1.1μM biotinylated monoclonal antibodies for 30 min at room temperature. HSQ-89 cells were seeded at 2.0×104cells per well in 96-well plates and cultured overnight. On the following day, they were exposed to various concentrations (0.054 pM∼4.2 nM) of either IT-Robo1 or IT-NC.
Related Products: Streptavidin-ZAP (Cat. #IT-27)