Shanahan SL, Kunder N, Inaku C, Hagan NB, Gibbons G, Mathey-Andrews N, Anandappa G, Soares S, Pauken KE, Jacks T, Schenkel JM (2024) Longitudinal intravascular antibody labeling identified regulatory t cell recruitment as a therapeutic target in a mouse model of lung cancer. J Immunol ji2400268. doi: 10.4049/jimmunol.2400268 PMID: 39082930
Objective: To develop an intravascular antibody technique to label circulating mouse leukocytes before they migrate to tissues, providing unprecedented insight into the kinetics of recruitment.
Summary: Leukocyte trafficking depended on the integrins CD11a/CD49d, and CD11a/CD49d blockade led to significant tumor burden reduction in mice. Preventing circulating Treg recruitment through depletion or sequestration in lymph nodes was sufficient to decrease tumor burden, indicating that Treg migration was crucial for suppressing antitumor immunity.
Usage: To deplete circulating Tregs, mice were treated every other day with 20mg of Anti-CD25 SAP (IT-29) for 6 days.
Related Products: Anti-CD25-SAP mouse (Cat. #IT-29)