Yamamoto-Fukuda T, Akiyama N, Tatsumi N, Okabe M, Kojima H (2022) Keratinocyte growth factor stimulates growth of p75+ neural crest lineage cells during middle ear cholesteatoma formation in mice. Am J Pathol 192(11):1573-1591. doi: 10.1016/j.ajpath.2022.07.010 PMID: 36210210
Objective: To identify the role of neural crest (NC) lineage epithelial cells during cholesteatoma formation and to detect specific cell markers to identify these NC lineage epithelial cells in human cholesteatoma tissues.
Summary: Cholesteatoma is a pathologic condition associated with otitis media and is a gradually destructive epithelial tumor within the middle ear and temporal bone. NC lineage epithelium controls the homeostasis of the middle ear and plays a role in repairing otitis media. Keratinocyte growth factor (KGF) stimulates epithelial cell growth and is associated with the recurrence of cholesteatomas. Injections of the immunotoxin mu p75-SAP (Cat. #IT-16) into the cholesteatoma tissue eliminated p75-postitive NC cells and diminished the cholesteatoma. This helped demonstrate that NC lineages are the cellular origin of cholesteatoma and that p75 transcription under KGF is required for the development of cholesteatoma. Controlling p75 signaling may reduce the proliferation of NC cells and could represent a new therapeutic target for cholesteatoma.
Usage: 10 uL mu-p75-SAP (0.2 or 0.6 mg/mL) was injected into the cholesteatoma region or tympanic membrane.
Related Products: mu p75-SAP (Cat. #IT-16)