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Intrathecal injections of saporin conjugated substance-P alter the development of renal hypertension.

Ciriello J, Rosas-Arellano MP, Solano-Flores LP (2000) Intrathecal injections of saporin conjugated substance-P alter the development of renal hypertension. Neuroscience 2000 Abstracts 310.2. Society for Neuroscience, New Orleans, LA.

Summary: We have recently shown that there is an up-regulation of mRNA encoding Substance-P (SP) in dorsal root ganglia in the two kidney, one clip (2K1C) model of renal hypertension. In this study, the effect of intrathecal injections of the toxin saporin conjugated to SP (SAP; 5 x 10-6 M) on the development of 2K1C hypertension was investigated in male Wistar rats. Rats were randomly assigned to 2 groups and instrumented with an intrathecal cannula that ended at the T10-T12 spinal level. Arterial pressure (AP) and heart rate (HR) were recorded using the indirect tail-cuff method. After a 1 week control period, one group of animals received a single 10 ┬Ál injection of SAP and the other an equal volume of the saline vehicle (control). Two weeks later a clip was placed on the left renal artery in all animals. Injections of either SAP or the vehicle did not alter AP and HR compared to pre-injection levels. In addition, AP and HR were not altered in the SAP group compared to controls for the 2 week period prior to the renal clip. However, during the 4 weeks after the renal clip, AP and HR in the SAP group were significantly lower compared to the control animals. These data suggest that dorsal horn neurons containing SP receptors and that receive afferent renal nerve inputs are involved in renal hypertension.

Related Products: SP-SAP (Cat. #IT-07)

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