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Development of a novel pipette tip-aided cell cloning method for the effective isolation of genome-edited porcine cell

Sato M, Saitoh I, Akasaka E, Inada E (2021) Development of a novel pipette tip-aided cell cloning method for the effective isolation of genome-edited porcine cell. OBM Genetics 5(1):16. doi: 10.21926/obm.genet.2101126

Summary: Isolation of clonal cells from a single colony is an essential step in the process of obtaining pure populations of stably-transfected clones after gene transfer and the subsequent drug selection. In the present study, a novel, simple, and non-invasive technique for the isolation of cells from single colonies using a disposable pipette tip was developed.

Usage: A toxin-based, drug-free selection system involving IB4-SAP was employed in the present study.

Related Products: IB4-SAP (Cat. #IT-10)

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