Birthelmer A, Dommes E, Jeltsch H, Cassel JC, Jackisch R (2002) Septal grafts and evoked acetylcholine release in the rat hippocampus after 192 IgG-saporin lesions. Neuroreport 13(7):973-976. doi: 10.1097/00001756-200205240-00015
Summary: The authors investigate the structural and behavioral effects of intrahippocampal grafts containing cholinergic neurons into a lesioned region of the brain. Previous studies in rats were complicated by the lack of a specific cholinergic lesioning agent. 0.4 µg 192-Saporin (Cat. #IT-01) in 0.4 µl was injected into the vertical limb of the diagonal band of Broca in rats, then 6 to 10 months later the animals received intrahippocampal grafts of septal cells containing cholinergic neurons. Measurement of noradrenaline and serotonin uptake indicate that the grafts were able to produce only modest cholinergic effects. The authors conclude that this may be a result of performing the graft too soon following administration of the immunotoxin.
Related Products: 192-IgG-SAP (Cat. #IT-01)