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Selective lesioning of nucleus incertus with corticotropin releasing factor-saporin conjugate.

Lee LC, Rajkumar R, Dawe GS (2014) Selective lesioning of nucleus incertus with corticotropin releasing factor-saporin conjugate. Brain Res 1543:179-190. doi: 10.1016/j.brainres.2013.11.021 PMID: 24287211

Objective: To investigate the role of Nucleus Incertus (NI) neurons in behavior by lesioning these neurons in rats using CRF-SAP (IT-13).

Summary: NI neurons are integral to CRF1, Relaxin-3, and GABA signaling pathways. By targeting the NI region with CRF-SAP, these neurons are selectively eliminated, allowing for the study of Relaxin-3’s influence on mood and behavior. Given the NI neurons’ involvement in emotional regulation, this approach sheds light on their contribution to mental illness and psychiatric disorders.

Usage: Infusion of 172 ng of CRF–SAP (IT-13) into the NI of rats. Blank-SAP (Cat. #IT-21) was used as a control.

Related Products: CRF-SAP (Cat. #IT-13), Blank-SAP (Cat. #IT-21)

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