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  4. Effects of hypocretin (orexin) neuronal loss on sleep and extracellular adenosine levels in the rat basal forebrain.

Effects of hypocretin (orexin) neuronal loss on sleep and extracellular adenosine levels in the rat basal forebrain.

Murillo-Rodriguez E, Liu M, Blanco-Centurion C, Shiromani PJ (2008) Effects of hypocretin (orexin) neuronal loss on sleep and extracellular adenosine levels in the rat basal forebrain. Eur J Neurosci 28(6):1191-1198. doi: 10.1111/j.1460-9568.2008.06424.x

Summary: Adenosine levels in the basal forebrain are thought to regulate the waxing and waning of sleep drive. Rats received bilateral 100-ng injections of orexin-SAP (Cat. #IT-20) into the lateral hypothalamus – resulting in a 94% loss of orexin-containing neurons. Lesioned animals displayed several changes in sleep characteristics, but no increase of adenosine levels after sleep deprivation. The results indicate that sleep changes due to orexin-SAP lesioning occur independently of adenosine levels.

Related Products: Orexin-B-SAP (Cat. #IT-20)