Murillo-Rodriguez E, Liu M, Blanco-Centurion C, Shiromani PJ (2008) Effects of hypocretin (orexin) neuronal loss on sleep and extracellular adenosine levels in the rat basal forebrain. Eur J Neurosci 28(6):1191-1198. doi: 10.1111/j.1460-9568.2008.06424.x
Summary: Adenosine levels in the basal forebrain are thought to regulate the waxing and waning of sleep drive. Rats received bilateral 100-ng injections of orexin-SAP (Cat. #IT-20) into the lateral hypothalamus – resulting in a 94% loss of orexin-containing neurons. Lesioned animals displayed several changes in sleep characteristics, but no increase of adenosine levels after sleep deprivation. The results indicate that sleep changes due to orexin-SAP lesioning occur independently of adenosine levels.
Related Products: Orexin-B-SAP (Cat. #IT-20)