How can I target B-cells? Which secondary conjugate should I use?
ATS makes secondary conjugates that use your primary antibody to target cells for elimination. Just mix your primary antibody with a secondary antibody conjugated to the ribosome-inactivating protein, Saporin, to screen your antibodies. The cells targeted by your primary antibody are eliminated.
Recognizes whole IgG
![whole IgG](https://atsbio.com/news/thumb/13q2_talk2.jpg)
![Hum-ZAP](https://atsbio.com/news/thumb/13q2_talk1.jpg)
Hum-ZAP (Cat. #IT-22) is made with
a bivalent secondary antibody
that recognizes whole IgG.
B-Cells have endogenous IgGs.
![Fab-ZAP](https://atsbio.com/news/thumb/13q2_talk3.jpg)
Fab-ZAP (Cat. #IT-51) is made with
a monovalent secondary antibody
that recognizes whole IgG.
B-Cells have endogenous IgGs.
Recognizes Fc region
![FC region IgG](https://atsbio.com/news/thumb/13q2_talk4.jpg)
![FabFc-ZAP](https://atsbio.com/news/thumb/13q2_talk5.jpg)
FabFc-ZAP (Cat. #IT-65) is made with
a monovalent secondary antibody
that recognizes ONLY the FC portion of IgG.