- We’re highlighting our ZAP antibody internalization kits and our line of secondary antibody saporin conjugates. We have given these products the moniker “ZAP” in place of saporin.
- Our ZAP conjugates consist of a variety of secondary antibodies that allow a large number of targeting agents to be screened quickly and cost-efficiently for specificity, functional binding, internalization, and EC50 determination.
- The conjugates are constructed using either species-specific secondary antibodies, or streptavidin (for use with biotinylated Targeting Agents), and they are chemically attached to Saporin, a potent plant ribosome-inactivating proteins.
- This article by Marquez et al. showcases how our secondary conjugates can be used to screen antibodies and then from that choose the best candidate to create a custom conjugation.
- Marquez J, Dong J, Dong C, Tian C, Serrero G (2021) Identification of prostaglandin F2 receptor negative regulator (PTGFRN) as an internalizable target in cancer cells for antibody-drug conjugate development. PLoS One 16(1):e0246197. doi: 10.1371/journal.pone.0246197
- PTGFRN is a cell-surface protein that is upregulated in certain cancer types, including head and neck and, notably, pediatric medulloblastoma, an aggressive cancer with limited therapeutic options. With the selection of the mouse monoclonal antibody 33B7, the authors identified PTGFRN as a potential therapy target, and were able to show that it is internalized by incubation with 33B7.
episode24, episode46