Congratulations to Dr. Argyle Bumanglag and the team at University of Florida for the important work they presented using SSP-SAP to create an animal model of temporal lobe epilepsy.
Injections of SSP-SAP into the hippocampus of a rat causes acute hippocampal injury, permanent dentate granule cell-onset epilepsy, and hippocampal sclerosis that closely resembles the selective hippocampal pathology exhibited by patients diagnosed with TLE. The rats are chronically epileptic, with data going out a year for lesioned animals.
Also see the recent publication:
Chun E, Bumanglag AV, Burke SN, & Sloviter RS. Targeted hippocampal GABA neuron ablation by Stable Substance P–saporin causes hippocampal sclerosis and chronic epilepsy in rats. (2019). Epilepsia, 60 (5):e52-e57.
Objective: Hippocampal GABA neurons were targeted for selective elimination to determine whether a focal hippocampal GABAergic defect in an otherwise normal brain can initiate cryptogenic temporal lobe epilepsy with hippocampal sclerosis.
Summary: Hippocampal GABAergic dysfunction is epileptogenic and can produce the defining features of cryptogenic temporal lobe epilepsy.
Dose: Intrahippocampal injections of SSP-SAP (0.4 ng/10 nL) were performed using a 0.5-μL Neuros Syringe lowered into four hippocampal sites along both the transverse and longitudinal hippocampal axes bilaterally.