Macrophage Targeting

The Critical Role of Il-10 in the Antineuroinflammatory and Antioxidative Effects of Rheum Tanguticum on Activated Microglia. Meng J, Ni J, Wu Z, Jiang M, Zhu A, Qing H, & Nakanishi H. (2018). Oxidative Medicine and Cellular Longevity, 2018 12.

Objective:  To investigate anti-inflammatory and antioxidative effects of a traditional Tibetan medicine (Rheum tanguticum; RT) on activated microglia.

Summary:  RT may be useful for the pharmacological intervention against excessive inflammatory and oxidative responses associated with AD by inducing the production of IL-10 by microglia.

DoseMac-1-SAP (Cat. #IT-06, 1.3nM) was applied to hippocampal slice cultures 24 h prior to stimulation with chromogranin A or pancreastatin.

Mac-1-SAP (Cat. #IT-06)

A tool for eliminating cells that express Mac-1 (CD11b) receptor in mouse or human; targeted via the antibody to CR3 (CD11b), eliminated via saporin.

CD11b is an alpha subunit of Mac-1, also known as CR3. CD11b is the receptor for the C3bi fragment of complement. This receptor is involved in bacterial phagocytosis. A reduction in neutrophil CD11b expression after severe traumatic injury correlates with increased septic complications. CD11b is a component of integrins, important for adhesion of neutrophils to surfaces. Mac-1-SAP recognizes the Mac-1 (CD11b) receptor in mouse and human. Mac-1-SAP is excellent for removing contaminating macrophages from primary cultures to determine their role(s) in autoimmune diseases and in degenerative diseases such as Alzheimer’s.

Mac-1-SAP eliminates Mac-1 positive (CD11b positive) cells. All other cells are left untouched.

To eliminate Mac-1-positive cells in rat, use Mac-1-SAP rat (Cat. #IT-33).