Agents of Interest in Pain Research
| Agent | Target | Lesioning Use |
|---|---|---|
| 192-IgG-SAP Cat. #IT-01 | p75NTR, low affinity NGFR (cholinergic basal forebrain, cerebellar Purkinje neurons, postganglionic autonomic neurons and some primary sensory neurons) | Immunolesioning |
| Anti-DBH-SAP Cat. #IT-03 | Dopamine beta hydroxylase (adrenergic and noradrenergic neurons) | Immunolesioning by application to either dendrites/soma or by retrograde axonal transport |
| Anti-SERT-SAP Cat. #IT-23 | Sertonin transporter (serotonergic neurons) | Immunolesioning |
| Dermorphin-SAP Cat. #IT-12 | mu opioid receptor (striatal neurons, lamina II dorsal horn nociceptive internerons, ventral medulla) | Direct application, spinal intrathecal injection |
| OX7-SAP Cat. #IT-02 | Thy-1 (all rat neurons and some T lymphocytes) | Suicide transport in both peripheral and central nervous systems |
| SSP-SAP Cat. #IT-11 | Neurokinin-1 receptor (NK-1R) (striatal cholinergic interneurons, dorsal horn nociceptive projection neurons) | Direct application to dendrites/soma, spinal intrathecal injection |
Note: Only antibody-toxin conjugates undergo retrograde transport
A) Normal immunoperoxidase staining for NK-1r after vehicle injection (arrows).
B) Decreased NK-1r staining after intrathecal administration of SP-SAP (175 ng).
Magnification bar in B indicates 100 μm. Counts of NK-1r-expressing neurons in deeper laminae revealed that cell loss was limited to the superficial laminae (I and II).