Antibody Screening for Cancer Therapeutics

Targeting of Embryonic Annexin A2 Expressed on Ovarian and Breast Cancer by the Novel Monoclonal Antibody 2448. (2018) Cua S, Tan HL, Fong WJ, Chin A, Lau A, Ding V, Song Z, Yang Y, & Choo A. Oncotarget, 9 (17):13206-13221. [PMID 29568351]

Objective: To develop mAbs to potentially target oncofetal antigens and be repurposed for antibody or antibody drug conjugate (ADC) therapy.

Summary: The novel IgG1, 2448, was shown to target a unique glycosylated surface epitope on ANXA2. As a possible therapeutic candidate for ovarian and breast cancer, 2448 demonstrated anti-tumor activity via two independent mechanisms of action.

Method: Cells were seeded in 96-well plates at 1000 or 2000 cells/well. Primary antibody, 2448 or ch2448 (10 μg/mL) was pre-mixed with appropriate secondary saporin conjugate, Mab-ZAP or Hum-ZAP.  The most significant decreases in cell viability (20% to 60%) were observed against the epithelial IGROV1 and MCF7 cell lines.  ATS created a Custom ADC by direct conjugation of saporin to ch2448 (ch2448-SAP).  As a control, an isotype chimeric IgG was also conjugated to saporin (IgG-SAP). Compared to using secondary saporin conjugates, ch2448-SAP induced and increase of  20–30% cytotoxicity.)


Mab-ZAP Antibody Internalization Kit (Cat. #KIT-04)

Mab-ZAP uses your primary mouse monoclonal IgG antibody to target and eliminate cells that recognize your primary antibody. This secondary conjugate uses the secondary antibody (affinity-purified goat anti-mouse IgG) to “piggyback” onto YOUR mouse primary antibody. Mab-ZAP can be utilized for screening mouse IgG antibodies for internalization and/or their suitability to make potent immunotoxins.

Three sizes are available; each kit includes assay reagents and the following aliquots of Mab-ZAP and control:

  • KIT-04-Z (tests 1 ab) includes 25 mcg each of Mab-ZAP and Control Goat-IgG-SAP
  • KIT-04-Z4 (tests 4 abs) includes 100 mcg each of Mab-ZAP and Control Goat-IgG-SAP
  • KIT-04-Z10 (tests 10 abs) includes 250 mcg each of Mab-ZAP and Control Goat-IgG-SAP

Hum-ZAP Antibody Internalization Kit (Cat. #KIT-22)

Hum-ZAP uses your primary human monoclonal IgG antibody to target and eliminate cells that recognize your primary antibody. This secondary conjugate uses the secondary antibody (affinity-purified goat anti-human IgG) to “piggyback” onto YOUR human primary antibody. Hum-ZAP can be utilized for screening human IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.

Three sizes are available; each kit includes assay reagents and the following aliquots of Hum-ZAP and control:

  • KIT-22-Z (tests 1 ab) includes 25 mcg each of Hum-ZAP and Control Goat-IgG-SAP
  • KIT-22-Z4 (tests 4 abs) includes 100 mcg each of Hum-ZAP and Control Goat-IgG-SAP
  • KIT-22-Z10 (tests 10 abs) includes 250 mcg each of Hum-ZAP and Control Goat-IgG-SAP