Leptin receptor-expressing neurons in the ventromedial nucleus of the hypothalamus contribute to weight loss caused by fourth ventricle leptin infusions. (2019). Am J Physiol Endocrinol Metab PMID: 31361549
Seamon M, Ahn W, Li A-J, Ritter S, & Harris RBS.
Objective: To test the importance of VMH leptin responsiveness in mediating weight loss caused by fourth ventricle leptin infusion.
Summary: Leptin did not inhibit food intake and respiratory exchange ratio in rats treated with Leptin-SAP. VMH leptin receptors do not play a significant role in maintaining energy balance in basal conditions, but limit weight gain during positive energy balance.
Dose: Bilateral VMH 75-nl injections of 260 ng/ml of Leptin-SAP or Blank-SAP.