FGF-2, or basic fibroblast growth factor, binds all of the FGF receptors with high affinity. We have used this molecule to produce FGF-SAP (Cat. #IT-38), which has a healthy experimental publication record (“FGF” and “saporin” in PubMed: 25 hits). It has been used to clean primary cultures of fibroblasts.[1] It was important in determining the role of smooth muscle cells in restenosis of damaged vasculature.[2] It was widely used in vivo for the elimination of FGF receptor-expressing cells, including neuronal cell types,[3] cancer cells,[4] and lens epithelial cells.[5] Figure 1 shows the cytotoxicity of ATS’ rat FGF-SAP conjugate to mouse NIH3T3 cells, with a potent ED50 of 68 pM. This conjugate will be useful for the study of systems biology.
References
- Beattie GM, Lappi DA, Baird A, Hayek A (1990) Diabetes 39:1002.
- Lindner V, Lappi DA, Baird A, Majack RA, Reidy MA (1991) Circulation Res 68:106.
- Gonzalez AM, Lappi DA, Buscaglia ML, Carman LS, Gage FH, Baird A (1991) Ann NY Acad Sci 638:442.
- Beitz JG, Davol-Lewis P, Clark JW, Kato J, Medina M, Frackelton AR, Lappi DA, Baird A, Calabresi P (1992) Cancer Res 52:227.
- David T, Tassin J, Lappi DA, Baird A, Courtois Y (1992) J Cell Physiol 153:483.
