Marasmius oreades agglutinin (MOA) derives from the Marasmius oreades mushroom. MOA consists of an intact 33 kDa and truncated 23 kDa subunit as well as a 10 kDa polypeptide. MOA has been used to study glomerular endothelial injury and to agglutinate B-type red blood cells.
This recombinant protein binds specifically to blood group B antigens and has a high affinity to alpha-Gal, a carbohydrate found on B-type blood cells. Recombinant MOA was expressed in E. coli and purified using affinity chromatography. This product is routinely tested by coomassie stain of a SDS-PAGE gel and agglutination assay.
Applications include red blood cell (RBC) agglutination.
keywords: Lectin, Marasmium oreades, agglutinin, mushroom, MOA, Galα1,3Gal, Galα1,3Galß1,4GlcNAc/Glc, carbohydrate, sugar, blood group B, thrombotic microangiopathies, glomerular endothelial injury, glycoproteins, 3T3 cells, biotin