Goat IgG-SAP is the perfect control for use with secondary conjugates. The secondary antibody conjugates are used with a bivalent primary antibody (a mouse monoclonal, rabbit polyclonal, human monoclonal, rat monoclonal, or IgM mouse monoclonal) to determine if the primary antibody can internalize saporin and would, therefore, be suitable for conjugation as a primary immunotoxin. Goat IgG-SAP, used in place of the secondary antibody conjugate, will give a definitive baseline for comparison of the activity of the primary antibody to internalize.
Goat IgG-SAP serves as a control for goat IgG-containing immunolesioning agents.
keywords: saporin, control, IgG, goat, whole
Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom
Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom. Toxins (Basel) 14(3):184. doi: 10.3390/toxins14030184 PMID: 35324681
Summary: Saporin is a ribosome-inactivating protein that can cause inhibition of protein synthesis and causes cell death when delivered inside a cell. Development of commercial Saporin results in a technology termed ‘molecular surgery’, with Saporin as the scalpel. Its low toxicity (it has no efficient method of cell entry) and sturdy structure make Saporin a safe and simple molecule for many purposes. The most popular applications use experimental molecules that deliver Saporin via an add-on targeting molecule. These add-ons come in several forms: peptides, protein ligands, antibodies, even DNA fragments that mimic cell-binding ligands. Cells that do not express the targeted cell surface marker will not be affected. This review will highlight some newer efforts and discuss significant and unexpected impacts on science that molecular surgery has yielded over the last almost four decades. There are remarkable changes in fields such as the Neurosciences with models for Alzheimer’s Disease and epilepsy, and game-changing effects in the study of pain and itch. Many other uses are also discussed to record the wide-reaching impact of Saporin in research and drug development.
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