Anti-CD150-SAP [IT-103, KIT-103]

a tool for eliminating cells that express the CD150 receptor in mice; targeted via the rat monoclonal antibody to mouse CD150 (clone TC15-12F12.2), eliminated via saporin 

   First time ordering this product? Consider purchasing a kit which also includes controls.

SKU: IT-103 Category: Quantity: Individual 25 ug, Individual 100 ug, Individual 250 ug, Individual 1 mg, Kit w/controls 25 ug, Kit w/controls 100 ug, Kit w/controls 250 ug | Antibody Type: Monoclonal | Host: rat | Reactivity: mouse | Conjugate: streptavidin-saporin | Usage: eliminates cells |

CD150, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is primarily expressed on immune cells, including thymocytes, activated T and B cell subsets, dendritic cells, and endothelial cells. CD150 expression is upregulated following cellular activation and is notably higher on Th1 cells and regulatory T cells, while it is reduced or absent in Th2 populations. Through interaction with intracellular signaling molecules such as SHP-2, CD150 plays a role in modulating immune responses, including enhancing IFN-γ production in Th1 cells. Functionally, CD150 is involved in T cell activation, cytokine signaling, and immune cell differentiation. Additionally, in combination with CD48, it serves as a valuable marker in the study of hematopoietic stem cells. 

This targeted toxin recognizes cells that express the CD150 receptor in mice. Anti-CD150-SAP is a bonded toxin between biotinylated rat monoclonal antibody to mouse CD150 (clone TC15-12F12.2) and the secondary conjugate Streptavidin-ZAP containing the ribosome-inactivating protein, saporin.  

Anti-CD150-SAP is available individually (Cat. #IT-103) or as a kit (Cat. #KIT-103) which includes Anti-CD150-SAP and BIgG-SAP rat (Cat. #IT-73).

keywords: cd150, slam, signaling lymphocyte activation molecule, IPO-3, T cells, B Cells hematopoietic stem cells, targeted conjugate, saporin

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Targeted depletion of dysfunctional hematopoietic stem cells mitigates myeloid-biased differentiation in aged mice

Ren X, Wang Y, Zhang Y (2025) Targeted depletion of dysfunctional hematopoietic stem cells mitigates myeloid-biased differentiation in aged mice. Cell Discov 11:56. doi: 10.1038/s41421-025-00810-3 PMID: 40490480

Objective: To develop and evaluate a targeted strategy for depleting dysfunctional, myeloid-biased CD150-high hematopoietic stem cells (HSCs) in aged mice to restore balanced hematopoiesis and mitigate aging-related blood disorders.

Summary: The study used an antibody-toxin conjugate to selectively eliminate CD150-high HSCs, improving lymphoid-to-myeloid ratios, reducing platelet hyperproduction, and restoring hematopoietic balance in aged mice. Treatment preserved functional CD150-low HSCs and showed minimal off-target or systemic toxicity.

Usage: Streptavidin-ZAP (IT-27) was combined with a biotinylated anti-CD150 antibody to generate Anti-CD150-SAP (IT-103). This conjugate was used at doses of 1–2 mg/kg in vivo and as low as 0.01 nM in vitro to specifically deplete CD150-high HSCs while sparing CD150-low populations.

Related Products: Streptavidin-ZAP (Cat. #IT-27), Anti-CD150-SAP (Cat. #IT-103)

https://youtube.com/shorts/V5vDAH65GzA?si=98CbkOcytvCDJd8o

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BIgG-SAP Rat (Cat. #IT-73)

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Cytotoxicity Assay for Targeted Toxins in vitro

Concentration Calculation Explained: Convert molarity to mg/ml and mg/ml to molarity

Preparing and Interpreting Cytotoxicity Data in vitro

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