Proteins come in all shapes and sizes and don’t always contain a ready-to-conjugate binding site. This and other variables can cause you to look toward one-step kits, often leaving you with un-purified and un-verified conjugate.
Let the experts in the field help provide you with the most effective conjugate while retaining the functionality of each of the components.
Contact us now to get started discussing the strategy that best meets your needs.
Why biotinylate through Advanced Targeting Systems?
What you get with a “One-Step-Kit”
- Un-purified conjugate
- Un-verified conjugate
- Estimated concentration
- Reacts only with amine groups
- >$590 to biotinylate up to 2mg material
ATS Custom Biotinylation
- Discuss options available for your protein
- Removal of unbound biotin & protein
- Calculation of ratio of moles biotin/moles protein
- Concentration verified by BCA*
- $525 to biotinylate 200 micrograms to 5 milligrams of material
- Zero hands-on time for you
*Dependent upon peptide, but typically available for most proteins such as antibodies.
Vice President and Director of Custom Conjugations at ATS, talks about important things to watch for to avoid compromising your next experiment.
The streptavidin-biotin interaction has found extensive use as a research tool. The bond created between streptavidin and biotin is rapid and essentially non-reversible, unaffected by most extremes of pH, organic solvents, and denaturing reagents. A variety of molecules including lectins, proteins, and antibodies, can be biotinylated and reacted with streptavidin-labeled probes or other detection reagents for use in biological assays.
It’s easy to test your biotinylated targeting agent using the Biotin-Z Internalization Kit. Each kit contains all the components needed to allow your targeting agent to be screened quickly and economically to provide specificity, functional binding, internalization, and EC50 determination.
The key component of the Biotin-Z Internalization Kit is Streptavidin-ZAP; Streptavidin attached to Saporin, the most potent (and safest for use in the laboratory) of the plant ribosome-inactivating proteins. Streptavidin-ZAP “piggybacks” onto YOUR biotinylated material in order to evaluate the ability of the reagent to internalize upon binding to its receptor. Using Streptavidin-ZAP and biotinylated targeting agents, specific cytotoxins can be created JUST BY MIXING!
You can also test your biotinylated targeting agent in vivo. Streptavidin-ZAP can be mixed with your biotinylated targeting agent and delivered specifically to eliminate cells that recognize your targeting agent.
Your scientific applications demand a purified and verified conjugation.
The experts at ATS can make your next conjugation a solid success with “zero” hands-on time for you.
Data sheets accompany all biotinylations and include:
- Protein Concentration Verified by Bicinchoninic Acid Assay (BCA assay)
- Average chemical ratio of moles of biotin to moles of protein
- Average effective molecular weight
Pricing: 200 micrograms to 5 milligrams of starting material …….$525.00 USD