The Neurokinin-1 (NK-1) receptor is a G-protein-coupled receptor characterized by seven transmembrane helices which preferentially binds the neuropeptide substance P. The NK-1 receptor (also known as the substance P receptor) plays a key role in pain and inflammation. Studies also implicate NK-1 receptors in depression and the growth of brain tumors.
This antibody recognizes the NK-1 receptor in rat, dog, and human. This antibody was developed in rabbit using a synthetic peptide corresponding to an amino acid sequence at the C-terminus of dog NK-1 receptor conjugated to bovine thyroglobulin with glutaraldehyde. The peptide sequence has a high degree of homology to other species such as human, mouse, rat and guinea pig. The antibody is routinely tested by immunohistochemistry and flow cytometry.
Applications include immunohistochemistry, flow cytometry, immunoblotting, and ELISA.
keywords: neurokinin-1 receptor, nk-1 receptor, nk-1r, nk1r, substance p receptor, SP, Anti-NK-1R, Anti-NK1R, Anti-SP, Anti-Neurokinin-1 Receptor, Anti-Substance P, pain, brain, neuroscience
Allopregnanolone suppresses mechanical allodynia and internalization of neurokinin-1 receptors at the spinal dorsal horn in a rat postoperative pain model
Fujita M, Fukuda T, Sato Y, Takasusuki T, Tanaka M (2018) Allopregnanolone suppresses mechanical allodynia and internalization of neurokinin-1 receptors at the spinal dorsal horn in a rat postoperative pain model. Korean J Pain 31:10-15. doi: 10.3344/kjp.2018.31.1.10. PMID: 29372021
Objective: To identify a new strategy for postoperative pain management, this study investigated the analgesic effects of allopregnanolone (Allo) in an incisional pain model, and its effects on the activities of the primary afferent fibers at the dorsal horn.
Summary: Systemic administration of Allopregnanolone inhibited mechanical allodynia and activities of the primary afferent fibers at the dorsal horn in a rat postoperative pain model. Allopregnanolone was proposed as a candidate for postoperative pain management.
Usage: Fluorescence immunohistochemistry: Six sections per animal were labeled with primary antiserum, rabbit anti-NK-1R (1:3000).
Related Products: NK-1 Receptor Rabbit Polyclonal, affinity-purified (Cat. #AB-N33AP)
Review
P2Y1 receptor-mediated potentiation of inspiratory motor output in neonatal rat in vitro.
Alvares T, Revill A, Huxtable A, Lorenz C, Funk G (2014) P2Y1 receptor-mediated potentiation of inspiratory motor output in neonatal rat in vitro. J Physiol 592:3089-3111. doi: 10.1113/jphysiol.2013.268136 PMID: 24879869
Summary: P2YR's are metabotropic purinergic receptors found in some parts of the CNS. A subtype of this receptor excites rhythm generating networks in the preBötzinger complex. In order to better understand the role of these receptors in modulation of motor output the authors used brainstem-spinal cord and medullary slice preparations from neonatal rats to investigate P2Y1R signaling on specific neurons that innervate diaphragm and airway muscles. Anti-NK1r (Cat. #AB-N33AP) at a 1:1000 dilution was used during the immunohistochemistry. The data suggest that loss of purinergic modulation contributes to motoneuron excitability.
Related Products: NK-1 Receptor Rabbit Polyclonal, affinity-purified (Cat. #AB-N33AP)
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