FITC-labeled Anti-SAP can be used to verify binding specificity of a targeted toxin to a cell line expressing the target molecule. By first binding the targeted toxin to fixed cells, then binding FITC-labeled Anti-SAP to the targeted toxin, specificity can be confirmed through the use of competing molecules or a control cell line.
Saporin was used as the immunogen. The antibody was affinity-purified against saporin attached to a CnBr-Sepharose support column. The affinity-purified polyclonal antiserum was conjugated to FITC (5-iodoacetamidofluorescein) using SPDP. The conjugate binds native and recombinant saporin.
Applications include flow cytometry.
Saporin Goat Polyclonal (Cat. #AB-15) | affinity-purified (Cat. #AB-15AP) | affinity-purified biotin-labeled (Cat. #AB-15AP-BT) | affinity-purified HRP-labeled (Cat. #AB-15AP-HRP) | affinity-purified Alexa488-labeled (Cat. #AB-15AP-FLA) | affinity-purified FITC-labeled (Cat. #AB-15AP-FL)
keywords: saporin, saponaria officinalis, plant enzyme, RIP, ribosome-inactivating protein, RIP I, internalization, cell death, Anti-Saporin, Anti-SAP, fluorescent, FITC