FITC-labeled Anti-SAP can be used to verify binding specificity of a targeted toxin to a cell line expressing the target molecule. By first binding the targeted toxin to fixed cells, then binding FITC-labeled Anti-SAP to the targeted toxin, specificity can be confirmed through the use of competing molecules or a control cell line.
Saporin was used as the immunogen. The antibody was affinity-purified against saporin attached to a CnBr-Sepharose support column. The affinity-purified polyclonal antiserum was conjugated to FITC (5-iodoacetamidofluorescein) using SPDP. The conjugate binds native and recombinant saporin.
Applications include flow cytometry.
Other Saporin Products:
Saporin Chicken Polyclonal, affinity-purified (Cat. #AB-17AP) | biotin-labeled (Cat. #AB-17APBT) | Alexa488-labeled (Cat. #AB-17APFLA)
Saporin Goat Polyclonal (Cat. #AB-15) | HRP-labeled (Cat. #AB-15HRP) | affinity-purified (Cat. #AB-15AP) | affinity-purified biotin-labeled (Cat. #AB-15APBT) | affinity-purified Alexa488-labeled (Cat. #AB-15APFLA) | affinity-purified FITC-labeled (Cat. #AB-15APFL)
Saporin Rabbit Polyclonal, affinity-purified (Cat. #AB-41AP)