pHast Conjugates
pHast Conjugates are one of our pHastest tools for quantitative testing of your primary antibody’s specificity, binding, and internalization, providing results in 1 day.
Fluorescence intensity increases over time, indicating internalization of the pHast conjugate. Data generated using an IncuCyte SX5.
How It Works
The pHast conjugate binds to your primary antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. pHast conjugates are fluorescent plate reader compatible and can be used to illuminate your lead antibody candidates in a 96-well plate format.
Contact us now to get started discussing the strategy that best meets your needs.
Available Conjugate Options
bivalent antibodies that
recognize the whole IgG
monovalent (Fab) antibodies that
recognize the whole IgG, without bivalent capping
monovalent (Fab) antibodies that recognize Fc region only
| Target | Recommended Product | Recommended Product | Recommended Product |
|---|---|---|---|
| alpaca/llama IgG (VHH domain) | Alpaca-pHast (PH-05) | ||
| chicken IgY | Chick-pHast (PH-06) | ||
| goat IgG | Goat-pHast (PH-07) | ||
| guinea pig IgG | gPIG-pHast (PH-08) | ||
| human IgG | Hum-pHast (PH-09) | Fab-pHast human (PH-01) | FabFc-pHast human (PH-10) |
| human IgM | Hug-M-pHast (PH-11) | FabFc-pHast Hug-M (PH-04) | |
| mouse IgG | Mab-pHast (PH-12) | Fab-pHast mouse (PH-02) | FabFc-pHast mouse (PH-13) |
| mouse IgM | Anti-M-pHast (PH-14) | ||
| rabbit IgG | Rab-pHast (PH-15) | Fab-pHast rabbit (PH-16) | |
| rat IgG | Rat-pHast (PH-17) | Fab-pHast rat (PH-18) | FabFc-pHast rat (PH-19) |
| | |
Also available:
Streptavidin-pHast
Streptavidin-pHast binds to your biotinylated protein and contains a pH-dependent fluorescent reporter (pHast). This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell.
