pHast Conjugates
pHast Conjugates are one of our pHastest tools for quantitative testing of your primary antibody’s specificity, binding, and internalization,
providing results in 1 day.
How It Works
The pHast conjugate binds to your primary antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. pHast conjugates are fluorescent plate reader compatible and can be used to illuminate your lead antibody candidates in a 96-well plate format.
Contact us now to get started discussing the strategy that best meets your needs.
Available Conjugate Options
 |  |  | |
|---|---|---|---|
| Target | made with bivalent antibodies that recognize the whole IgG | made with monovalent (Fab) antibodies that recognize the whole IgG, without bivalent capping | made with monovalent (Fab) antibodies that recognize Fc region only |
| alpaca/llama IgG (VHH domain) | Alpaca-pHast (PH-05) | ||
| chicken IgY | Chick-pHast (PH-06) | ||
| goat IgG | Goat-pHast (PH-07) | ||
| guinea pig IgG | gPIG-pHast (PH-08) | ||
| human IgG | Hum-pHast (PH-09) | Fab-pHast human (PH-01) | FabFc-pHast human (PH-10) |
| human IgM | Hug-M-pHast (PH-11) | FabFc-pHast Hug-M (PH-04) | |
| mouse IgG | Mab-pHast (PH-12) | Fab-pHast mouse (PH-02) | FabFc-pHast mouse (PH-13) |
| mouse IgM | Anti-M-pHast (PH-14) | ||
| rabbit IgG | Rab-pHast (PH-15) | Fab-pHast rabbit (PH-16) | |
| rat IgG | Rat-pHast (PH-17) | Fab-pHast rat (PH-18) | FabFc-pHast rat (PH-19) |
Also available:
Streptavidin-pHast
Streptavidin-pHast binds to your biotinylated protein and contains a pH-dependent fluorescent reporter (pHast). This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell.
Monitor Internalization Via Fluorescent Microscopy
Biotinylated Isolectin B4 mixed with Streptavidin-pHast (100 nM), then incubated overnight on rat kidney cells, shows detailed fluorescence and confirmation of internalization.