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Lack of phenotypical and morphological evidences of endothelial to hematopoietic transition in the murine embryonic head during hematopoietic stem cell emergence.
Iizuka K, Yokomizo T, Watanabe N, Tanaka Y, Osato M, Takaku T, Komatsu N (2016) Lack of phenotypical and morphological evidences of endothelial to hematopoietic transition in the murine embryonic head during hematopoietic stem cell emergence. PLoS One 11:e0156427. doi: 10.1371/journal.pone.0156427 PMID: 27227884
Summary: Hemogenic endothelial cells have been observed in several embryonic tissues, such as the dorsal aorta, the placenta and the yolk sac. Recent work also suggest that the mouse embryonic head also produces hematopoietic stem cells (HSCs)/progenitors. However, a histological basis for HSC generation in the head hasn’t been determined because the hematopoietic cluster and hemogenic endothelium have not been well characterized. The authors in this study used whole-mount immunostaining and 3D confocal reconstruction techniques to analyze both c-Kit hematopoietic cluster and Runx1 hemogenic endothelium in the whole-head vasculature. Alexa488 labeled anti-NGFr (Cat. #FL-N01AP) was used in flow cytometry. The number of c-Kit hematopoietic cells was 20-fold less in the head arteries than in the dorsal aorta. In addition, nascent hematopoietic cells, observed by a budding structure and a Runx1 hemogenic endothelium, were not observed in the head. These results suggest that head HSCs may not be or are rarely generated from the endothelium in the same manner as aortic HSCs.
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified Alexa488-labeled (Cat. #AB-N01APFLA)
Polyurethane/polylactide-blend films doped with zinc ions for the growth and expansion of human olfactory ensheathing cells (OECs) and adipose-derived mesenchymal stromal stem cells (ASCs) for regenerative medicine applications
Marycz K, Marędziak M, Grzesiak J, Szarek D, Lis A, Laska J (2016) Polyurethane/polylactide-blend films doped with zinc ions for the growth and expansion of human olfactory ensheathing cells (OECs) and adipose-derived mesenchymal stromal stem cells (ASCs) for regenerative medicine applications. Polymers (Basel 8(5):175. doi: 10.3390/polym8050175 PMID: 30979270
Objective: To show that polyurethane/polylactide blends (PU/PLDL) may be further improved by the addition of ZnO nanoparticles for the delivery of bioactive zinc oxide for cells.
Summary: The researchers showed that PU/PLDL blends doped with 0.001% of ZnO exert beneficial influence on adipose stromal stem cells and olfactory ensheathing cells in vitro.
Usage: Immunofluorescence staining to verify the p75+/GFAP+ phenotype of olfactory ensheathing cells (1:1000)
Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Efficient generation of human embryonic stem cell-derived corneal endothelial cells by directed differentiation
McCabe KL, Kunzevitzky NJ, Chiswell BP, Xia X, Goldberg JL, Lanza R (2015) Efficient generation of human embryonic stem cell-derived corneal endothelial cells by directed differentiation. PLoS One 10(12):e0145266. doi: 10.1371/journal.pone.0145266 PMID: 26689688
Usage: For immunostaining of the expression of Zona Occludens protein 1 (ZO-1), von Willebrand factor (vWF), p75/NGFR and CD31, traditional methods were utilized. Anti-NGFr (p75) 1:100.
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Treatment efficacy of NGF nanoparticles combining neural stem cell transplantation on Alzheimer’s Disease model rats.
Chen Y, Pan C, Xuan A, Xu L, Bao G, Liu F, Fang J, Long D (2015) Treatment efficacy of NGF nanoparticles combining neural stem cell transplantation on Alzheimer’s Disease model rats. Med Sci Monit 21:3608-3615. doi: 10.12659/msm.894567
Summary: NSC (neural stem cell) transplants into animals have been shown to compensate for the loss of cholinergic cells in the basal forebrain, a hallmark of Alzheimer’s disease. One hurdle to overcome is the actuation of NSC differentiation into the specific replacement cells needed. NGF has been shown to induce this differentiation, but it has a very short half-life and does not permeate tissue very effectively. In this work the authors administered 5 mcl of icv 192-IgG-SAP (Cat. #IT-01) to rats, followed by a graft of NCSs in the presence of NGF nanoparticles with a polymer coating. Rats receiving both NCSs and NGF nanoparticles showed significantly improved memory and learning functions as compared to control animals.
Related Products: 192-IgG-SAP (Cat. #IT-01)
The specification and maturation of nociceptive neurons from human embryonic stem cells
Boisvert EM, Engle SJ, Hallowell SE, Liu P, Wang ZW, Li XJ (2015) The specification and maturation of nociceptive neurons from human embryonic stem cells. Sci Rep 5:16821. doi: 10.1038/srep16821 PMID: 26581770
Usage: Immunocytochemistry 1:200
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)
Effects of brain-derived neurotrophic factor-pretreated neuron stem cell transplantation on Alzheimer’s disease model mice
Li T, Yu Y, Cai H (2015) Effects of brain-derived neurotrophic factor-pretreated neuron stem cell transplantation on Alzheimer’s disease model mice. Int J Clin Exp Med 8:21947-21955.
Summary: In order to generate the AD mouse model, mu p75-SAP (1-1.2 μg/μL) was injected to the bilateral icv areas.
Related Products: mu p75-SAP (Cat. #IT-16)
Neuroprotective effects of placenta-derived mesenchymal stem cell for rat model of dementia
Lee J, Jeong D, Chang W, Chang J (2015) Neuroprotective effects of placenta-derived mesenchymal stem cell for rat model of dementia. Neuroscience 2015 Abstracts 626.12/AA3. Society for Neuroscience, Chicago IL.
Summary: Introduction: The neuroprotective effects of mesenchymal stem cell (MSC) in neurodegenerative disease have been recently reported. In contrast of the transplantation effect of MSC derived from bone marrow, adipose tissue and human umbilical cord blood, the study of placenta-derived mesenchymal stem cell (pMSC) is still little known. In this study, we studied the effective method of placenta-derived mesenchymal stem cell (pMSC) transplantation by comparing intracerebroventrical (icv) with intravenous (iv) injection. In addition, we also tried to compare the effect of pMSC transplantation and standard treatment for dementia. Materials and Methods: We used the rat model of dementia by damaging basal forebrain cholinergic neurons using 192 IgG-saporin. 1 week after administration of 192 IgG-saporin, pMSC was injected via intraventricular route (icv, 1.2 x 106 cells/ul) or intravenous route (iv, 5 x 106 cells/200 ul) and Cyclosporine (immunosuppressant drug) were administrated at peritoneal cavity for preventing immune reaction by innate immunity (daily / 5 weeks). To compare the effect of stem cell therapy and standard therapy, some rats were treated with donepezil and 5 weeks after transplantation, all animals were tested visuo-spatial cognitive functions by Morris water maze. Results: The probe test of water maze, performance of pMSC transplantation group and donepezil group increased time spent in target quadrant and in platform zone. Also acetylcholinesterase(AChE) activity was increased in the hippocampus and medial prefrontal cortex(mPFC). Interestingly, iv group showed more improved behavior performance and acetylcholinesterase(AChE) activity than icv group. Immunohistochemistry of stem121 marker of stem cell and iba1 maker of microglia and Western blot of DCX and BDNF were also suggested the beneficial effect of both stem cell therapy and standard donepezil treatment. Conclusions: Our result show that pMSC recover spatial memory of dementia model by increasing acetylcholinesterase(AChE) activity. Intravenous injection of pMSC seemed to be more beneficial route for both risk managing and symptom improvement. And pMSC transplantation also showed similar efficacy of the donepezil for improving cognitive function. For determining the superiority of both treatment, further investigation should be needed. Acknowledgements: This work was supported (YonseiChallenge) by the Yonsei University Future-leading Research Initiative of 2014 and CABMC (Control of Animal Brain using MEMS Chip) funded by Defense Acquisition Program Administration (UD140069ID).
Related Products: 192-IgG-SAP (Cat. #IT-01)
Phenotypic and functional characterization of Bst+/- mouse retina.
Riazifar H, Sun G, Wang X, Rupp A, Vemaraju S, Ross-Cisneros F, Lang R, Sadun A, Hattar S, Guan M, Huang T (2015) Phenotypic and functional characterization of Bst+/- mouse retina. Dis Model Mech 8:969-976. doi: 10.1242/dmm.018176 PMID: 26035379
Summary: The belly spot and tail mutant mouse strain was first reported on in 1976. Among other phenotypic changes, it carries ocular mutations including retinal colobomas, reduced retinal ganglion cells (RGCs), and axon misrouting. In order to assess the use of this strain as a murine model for stem cell therapies of retinal degenerative diseases the authors performed a number of characterization experiments including electron microscopy, immunohistochemistry, testing of circadian rhythms, and morphological studies. Some of the immunohistochemistry was done using Anti-Melanopsin (Cat. #AB-N38) at a 1:5000 dilution.
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
Light-controlled endosomal escape of the novel CD133-targeting immunotoxin AC133-saporin by photochemical internalization – A minimally invasive cancer stem cell-targeting strategy.
Bostad M, Olsen C, Peng Q, Berg K, Høgset A, Selbo P (2015) Light-controlled endosomal escape of the novel CD133-targeting immunotoxin AC133-saporin by photochemical internalization – A minimally invasive cancer stem cell-targeting strategy. J Control Release 206:37-48. doi: 10.1016/j.jconrel.2015.03.008
Summary: Previously the authors demonstrated the use of photochemical internalization of a custom conjugate consisting of a CD133 antibody coupled to saporin (ATS Custom conjugation). Several cancer cell lines were plated, and incubated in the presence of a photosensitizer with either CD133-SAP at 8.6 pM or Saporin (Cat. #PR-01) at 24 pM. The different concentrations equalized the number of saporin molecules in each sample. A light source was used to initiate the internalization of the molecules. The results indicate that this is a viable strategy for the targeted treatment of cancer stem cells.
Related Products: Anti-CD133-SAP (Cat. #IT-82), Saporin (Cat. #PR-01), Custom Conjugates
Human embryonic stem cells form functional thyroid follicles
Ma R, Latif R, Davies TF (2015) Human embryonic stem cells form functional thyroid follicles. Thyroid doi: 10.1089/thy.2014.0537 PMID: 25585054
Summary: Lysates were prepared from single- and double-transfected cells by treating with 1 ·RIPA buffer for 20 min on ice. The samples were then centrifuged and treated with 5 · sample buffer at 100°C for 5 min and loaded on a 4–15% SDS-PAGE gel. Western blotting was performed according to a standard protocol.
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