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Neuroprotective effects of placenta-derived mesenchymal stem cell for rat model of dementia

Lee J, Jeong D, Chang W, Chang J (2015) Neuroprotective effects of placenta-derived mesenchymal stem cell for rat model of dementia. Neuroscience 2015 Abstracts 626.12/AA3. Society for Neuroscience, Chicago IL.

Summary: Introduction: The neuroprotective effects of mesenchymal stem cell (MSC) in neurodegenerative disease have been recently reported. In contrast of the transplantation effect of MSC derived from bone marrow, adipose tissue and human umbilical cord blood, the study of placenta-derived mesenchymal stem cell (pMSC) is still little known. In this study, we studied the effective method of placenta-derived mesenchymal stem cell (pMSC) transplantation by comparing intracerebroventrical (icv) with intravenous (iv) injection. In addition, we also tried to compare the effect of pMSC transplantation and standard treatment for dementia. Materials and Methods: We used the rat model of dementia by damaging basal forebrain cholinergic neurons using 192 IgG-saporin. 1 week after administration of 192 IgG-saporin, pMSC was injected via intraventricular route (icv, 1.2 x 106 cells/ul) or intravenous route (iv, 5 x 106 cells/200 ul) and Cyclosporine (immunosuppressant drug) were administrated at peritoneal cavity for preventing immune reaction by innate immunity (daily / 5 weeks). To compare the effect of stem cell therapy and standard therapy, some rats were treated with donepezil and 5 weeks after transplantation, all animals were tested visuo-spatial cognitive functions by Morris water maze. Results: The probe test of water maze, performance of pMSC transplantation group and donepezil group increased time spent in target quadrant and in platform zone. Also acetylcholinesterase(AChE) activity was increased in the hippocampus and medial prefrontal cortex(mPFC). Interestingly, iv group showed more improved behavior performance and acetylcholinesterase(AChE) activity than icv group. Immunohistochemistry of stem121 marker of stem cell and iba1 maker of microglia and Western blot of DCX and BDNF were also suggested the beneficial effect of both stem cell therapy and standard donepezil treatment. Conclusions: Our result show that pMSC recover spatial memory of dementia model by increasing acetylcholinesterase(AChE) activity. Intravenous injection of pMSC seemed to be more beneficial route for both risk managing and symptom improvement. And pMSC transplantation also showed similar efficacy of the donepezil for improving cognitive function. For determining the superiority of both treatment, further investigation should be needed. Acknowledgements: This work was supported (YonseiChallenge) by the Yonsei University Future-leading Research Initiative of 2014 and CABMC (Control of Animal Brain using MEMS Chip) funded by Defense Acquisition Program Administration (UD140069ID).

Related Products: 192-IgG-SAP (Cat. #IT-01)

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