Dadashkhan S, Irani S, Bonakdar S, Ghalandari B (2021) P75 and S100 gene expression induced by cell-imprinted substrate and beta-carotene to nerve tissue engineering. Journal of Applied Polymer Science 138(26):50624. doi: 10.1002/app.50624

Objective: Aim to differentiate adipose-derived stem cells (ADSCs) to Schwann cells (SCs), as one of the major cell sources in nerve regeneration.

Summary: Use of synergistic application of imprinting method and beta-carotene BC. Schwann cell imprinted substrates can mimic the morphology and topography of SCs and induce differentiation signals in mesenchymal stem cells. BC stimulates neural differentiation.

Usage: Immunocytochemistry

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Wang D, Shao X, Wang Q, Pan X, Dai Y, Yao S, Yin T, Wang Z, Zhu J, Xi X, Chen Z, Chen S, Zhang G (2021) Activated factor X targeted stored in platelets as an effective gene therapy strategy for both hemophilia A and B. Clin Transl Med 11(3):e375. doi: 10.1002/ctm2.375

Summary: Treatment of hemophiliacs with inhibitors remains challenging, and new treatments are in urgent need. Coagulation factor X plays a critical role in downstream blood coagulation cascade, which could serve as a bypassing agent for hemophilia therapy. Target expression of the FXa precursor to platelets can generate a storage pool of FXa in platelet α-granules, the platelet-stored FXa is effective in treating HA and HB with inhibitors, suggesting that this could be a novel choice for hemophilia patients with inhibitors.

Usage: A single dose of CD45.2-SAP (biotinylated Anti-CD45 mixed with Streptavidin-ZAP) enabled efficient engraftment of donor cells (> 90%) and full correction of sickle-cell anemia. (3 mg/kg iv; Palchaudhuri et al.).

See: Palchaudhuri R et al. Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nat Biotechnol 34:738-745, 2016.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Li Y, Lv S, Yuan H, Ye G, Mu W, Fu Y, Zhang X, Feng Z, He Y, Chen W (2021) Peripheral nerve regeneration with 3D printed bionic scaffolds loading neural crest stem cell derived Schwann cell progenitors. Advanced Functional Materials 31(16):2010215. doi: 10.1002/adfm.202010215

Objective: Regeneration efficiency and behavior of peripheral nerves are compared under three treatment strategies: 1) transplantation of Schwann cell progenitors induced from purified neural crest stem cells; 2) implantation of a multiscale scaffold based on high-resolution 3D printing; and 3) implantation of this bionic scaffold loading Schwann cell progenitors.

Summary: Implantation of multiscale scaffolds preloaded with neural crest stem cell derived Schwann cell progenitors is the best strategy to promote peripheral nerve regeneration with improved anatomy and function among the three different strategies.

Usage: Immunostaining (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Koeniger T, Bell L, Mifka A, Enders M, Hautmann V, Mekala SR, Kirchner P, Ekici AB, Schulz C, Wörsdörfer P, Mencl S, Kleinschnitz C, Ergün S, Kuerten S (2021) Bone marrow-derived myeloid progenitors in the leptomeninges of adult mice. Stem Cells 39(2):227-239. doi: 10.1002/stem.3311

Summary: This report confirms the presence of myeloid progenitors at the meningeal border of the brain and lays the foundation to unravel their possible functions in CNS surveillance and local immune cell production. Compared to bone marrow transfer after whole-body irradiation, chimerism developed more slowly in the CD45-SAP (biotinylated anti-CD45 mixed with Streptavidin-ZAP) model and only reached around 50% in the blood myeloid compartment 15 weeks after transplantation.

See: Palchaudhuri R et al. Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nat Biotechnol 34:738-745, 2016.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Motohashi T, Kawamura N, Watanabe N, Kitagawa D, Goshima N, Kunisada T (2020) Sox10 functions as an inducer of the direct conversion of keratinocytes into neural crest cells. Stem Cells Dev 29(23):1510-1519. doi: 10.1089/scd.2020.0106 PMID: 33040687

Objective: To investigate whether Sox10 functions in the direct conversion of other somatic cells into neural crest cells (NCCs).

Summary: Results demonstrate that Sox10 functions as an inducer of direct conversion into NCCs in cooperation with the transcription factors involved in NCC generation.

Usage: Flow cytometry and cell sorting

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Persaud SP, Ritchey JK, Choi J, Ruminski PG, Cooper ML, Rettig MP, DiPersio JF (2020) Antibody-drug conjugates targeting CD45 plus Janus kinase inhibitors effectively condition for allogeneic hematopoietic stem cell transplantation. bioRxiv 2020.10.02.324475. doi: 10.1101/2020.10.02.324475

Related Products: Streptavidin-ZAP (Cat. #IT-27), Blank-Streptavidin-SAP (Cat. #IT-27B)

Wu X, Cao Z, Chen H, Ou Q, Huang X, Wang Y (2020) Downregulation of Linc-RNA activator of myogenesis lncRNA participates in FGF2-mediated proliferation of human periodontal ligament stem cells. J Periodontol 91(3):422-427. doi: 10.1002/JPER.19-0317 PMID: 31378921

Usage: Western (1:1200)

Related Products: Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)

Ali A, Syed SM, Jamaluddin MFB, Colino-Sanguino Y, Gallego-Ortega D, Tanwar PS (2020) Cell lineage tracing identifies hormone-regulated and wnt-responsive vaginal epithelial stem cells. Cell Rep 30(5):1463-1477.e7. doi: 10.1016/j.celrep.2020.01.003 PMID: 32023462

Objective: To learn more about the role of Wnt signaling in vaginal epithelium (VE) homeostasis.

Summary: Data define heterogeneity in VE and identify VE stem cells. It was shown that canonical Wnt/b-catenin signaling is required for the proliferation and differentiation of VE stem cells.

Usage: histology and immunostaining (1:250)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Gao Y, Gao B, Zhu H, Yu Q, Xie F, Chen C, Li Q (2019) Adipose-derived stem cells embedded in platelet-rich plasma scaffolds improve the texture of skin grafts in a rat full-thickness wound model. Burns 46(2):377-385. doi: 10.1016/j.burns.2019.07.041 PMID: 31852618

Usage: western (1:500)

Related Products: Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)

Lee J, Seo Y, Shin J, Kong C, Na Y, Chang W, Chang J (2019) Increased transplantation efficacy of mesenchymal stem cell by focused ultrasound and improvement of the spatial memory in the 192 IgG-saporin rat model. Neuroscience 2019 Abstracts 048.01. Society for Neuroscience, Chicago, IL.

Summary: Introduction: Stem cell therapy has been found to have therapeutic effects in neurodegenerative disease, but traditional transplant methods, such as parenchymal or intravenous injection, possess limitations like secondary injuries, infection, and low survival rate of stem cells in the brain. Meanwhile, recently the focused ultrasound(FUS) was found to have promising results regarding transplantation of stem cells into the rat brain. However, the mechanism of stem cell transplantation with FUS and possibility of cognitive recovery remain elusive. Therefore, this study investigates a possibility for non-invasive focused ultrasound use in stem cell transplantation into the brain of dementia rat model. Materials & methods: We divided rats into five groups: Normal, Lesion, Cell only, FUS + Cell, and FUS only. We used 192 IgG-saporin for degeneration of basal forebrain cholinergic neuron and it was injected into all rats except for the normal group. After a week, 5p mesenchymal stem cells (MSC: 3*106/200ul) were injected in the tail vein of all rats of the cell only and FUS + Cell group, and the FUS + Cell group received the FUS three hours before cell transplantation. FUS was applied with parameters of 0.25Mpa, 300s (Targeted hippocampal region: AP -3.5, ML ±2). And last, FUS only group was received only FUS without any treatment. Five weeks after transplantation, rats performed the Morris water maze test. Results: MSC were detected in both cell only and FUS + Cell group of the hippocampus region. After comparing FUS+MSC & cell only rats, it was confirmed that FUS increases MSC homing in the sonicated rat’s brain. In addition, the most effective memory restoration occurred in the FUS + Cell group. Moreover, the FUS + Cell group exhibited better recall of the platform position than the other groups. And FUS only group did not recover. Conclusion: Noninvasive FUS can increase the efficacy of stem cell delivery. And memory impairment due to cholinergic denervation can be effectively improved by cell transplantation with FUS. The results of this study suggest possibility of stem cell homing and therapeutic effects of the FUS in dementia rat model. However, further study regarding the function of stem cells transplanted in the brain and a more detailed mechanism of stem cell homing by FUS is needed.

Related Products: 192-IgG-SAP (Cat. #IT-01)