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Increased transplantation efficacy of mesenchymal stem cell by focused ultrasound and improvement of the spatial memory in the 192 IgG-saporin rat model

Lee J, Seo Y, Shin J, Kong C, Na Y, Chang W, Chang J (2019) Increased transplantation efficacy of mesenchymal stem cell by focused ultrasound and improvement of the spatial memory in the 192 IgG-saporin rat model. Neuroscience 2019 Abstracts 048.01. Society for Neuroscience, Chicago, IL.

Summary: Introduction: Stem cell therapy has been found to have therapeutic effects in neurodegenerative disease, but traditional transplant methods, such as parenchymal or intravenous injection, possess limitations like secondary injuries, infection, and low survival rate of stem cells in the brain. Meanwhile, recently the focused ultrasound(FUS) was found to have promising results regarding transplantation of stem cells into the rat brain. However, the mechanism of stem cell transplantation with FUS and possibility of cognitive recovery remain elusive. Therefore, this study investigates a possibility for non-invasive focused ultrasound use in stem cell transplantation into the brain of dementia rat model. Materials & methods: We divided rats into five groups: Normal, Lesion, Cell only, FUS + Cell, and FUS only. We used 192 IgG-saporin for degeneration of basal forebrain cholinergic neuron and it was injected into all rats except for the normal group. After a week, 5p mesenchymal stem cells (MSC: 3*106/200ul) were injected in the tail vein of all rats of the cell only and FUS + Cell group, and the FUS + Cell group received the FUS three hours before cell transplantation. FUS was applied with parameters of 0.25Mpa, 300s (Targeted hippocampal region: AP -3.5, ML ±2). And last, FUS only group was received only FUS without any treatment. Five weeks after transplantation, rats performed the Morris water maze test. Results: MSC were detected in both cell only and FUS + Cell group of the hippocampus region. After comparing FUS+MSC & cell only rats, it was confirmed that FUS increases MSC homing in the sonicated rat’s brain. In addition, the most effective memory restoration occurred in the FUS + Cell group. Moreover, the FUS + Cell group exhibited better recall of the platform position than the other groups. And FUS only group did not recover. Conclusion: Noninvasive FUS can increase the efficacy of stem cell delivery. And memory impairment due to cholinergic denervation can be effectively improved by cell transplantation with FUS. The results of this study suggest possibility of stem cell homing and therapeutic effects of the FUS in dementia rat model. However, further study regarding the function of stem cells transplanted in the brain and a more detailed mechanism of stem cell homing by FUS is needed.

Related Products: 192-IgG-SAP (Cat. #IT-01)