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Bronchoconstrictor effect of the tachykinin NK₃-receptor agonists [MePhe⁷]-neurokinin B and senktide in the isolated guinea pig lung
Corboz MR, Rivelli MA, Eckel SP (2010) Bronchoconstrictor effect of the tachykinin NK₃-receptor agonists [MePhe⁷]-neurokinin B and senktide in the isolated guinea pig lung. Exp Lung Res 36(9):509-521. doi: 10.3109/01902141003777582
Related Products: NKB-SAP (Cat. #IT-63)
Dorsal horn neurons expressing NK-1 receptors mediate scratching in rats.
Carstens EE, Carstens MI, Simons CT, Jinks SL (2010) Dorsal horn neurons expressing NK-1 receptors mediate scratching in rats. Neuroreport 21:303-308. doi: 10.1097/WNR.0b013e328337310a
Summary: The itch signal is passed through the superficial dorsal horn. The authors investigated whether ablation of NK-1 receptor-expressing neurons in this area would affect itch-related scratching behavior. Rats received 20 µl of 2.27-µM SP-SAP (Cat. #IT-07) as an intracisternal injection. The reduction in itch response to intradermal 5-hydroxytryptamine indicates that NK-1 receptor-expressing superficial dorsal horn neurons are important for spinal itch transmission.
Related Products: SP-SAP (Cat. #IT-07)
Targeted ablation of mesenteric projecting sympathetic neurons reduces the hemodynamic response to pain in conscious spinal cord transected rats.
Lujan HL, Palani G, Peduzzi J, Dicarlo SE (2010) Targeted ablation of mesenteric projecting sympathetic neurons reduces the hemodynamic response to pain in conscious spinal cord transected rats. Am J Physiol Regul Integr Comp Physiol 298(5):R1358-1365. doi: 10.1152/ajpregu.00755.2009
Summary: Autonomic dysreflexia is a life-threatening hypertension as a result of a spinal cord injury above thoracic level 6. The authors investigated whether reduction of sympathetic activity can reduce the severity of this condition. Rats received 13.5 µg injections of CTB-SAP (Cat. #IT-14) into the celiac ganglion resulting in ablation of mesenteric projecting sympathetic neurons. Lesioned animals displayed a reduced pressor response to pain after spinal cord transection, to some extent ameliorating autonomic dysreflexia.
Related Products: CTB-SAP (Cat. #IT-14)
Unique contributions of distinct cholinergic projections to motor cortical plasticity and learning.
Conner JM, Kulczycki M, Tuszynski MH (2010) Unique contributions of distinct cholinergic projections to motor cortical plasticity and learning. Cereb Cortex 20(11):2739-2748. doi: 10.1093/cercor/bhq022
Summary: This work mapped the basal cholinergic forebrain system associations with skilled motor learning and motor function recovery after cortical injury. Rats were lesioned with 192-IgG-SAP (Cat. #IT-01). The animals received either two rostrocaudal injections of 75-112 ng; two 19 ng injections into the “prefrontal depletion site”; or two 19 ng injections into the “motor cortex depletion site.” Loss of motor cortex cholinergic systems disrupts map plasticity and skilled motor behavior, indicating that control of these systems rests within the motor cortex.
Related Products: 192-IgG-SAP (Cat. #IT-01)
The brainstem noradrenergic systems in stress, anxiety, and depression.
Itoi K, Sugimoto N (2010) The brainstem noradrenergic systems in stress, anxiety, and depression. J Neuroendocrinol 22(5):355-361. doi: 10.1111/j.1365-2826.2010.01988.x
Summary: In this review the authors examine the relationship between the central noradrenergic system, fear/anxiety states, and depression. The use of anti-DBH-SAP (Cat. #IT-03) to investigate the function of the noradrenergic system in these paradigms is described.
Related Products: Anti-DBH-SAP (Cat. #IT-03)
Targeted ablation of cardiac sympathetic neurons reduces the susceptibility to ischemia-induced sustained ventricular tachycardia in conscious rats.
Lujan HL, Palani G, Zhang L, Dicarlo SE (2010) Targeted ablation of cardiac sympathetic neurons reduces the susceptibility to ischemia-induced sustained ventricular tachycardia in conscious rats. Am J Physiol Heart Circ Physiol 298:H1330-H1339. doi: 10.1152/ajpheart.00955.2009
Summary: It has been shown that reduction of cardiac sympathetic activity protects against ventricular tachy-arrhythmias, which are the leading cause of death in industrially developed countries. Rats received 10 µg injections of CTB-SAP (Cat. #IT-14) into each stellate ganglia. Using comparison of ventricular tachycardia onset times after coronary artery occlusion it was found that lesioned rats were less susceptible to tachycardia events.
Related Products: CTB-SAP (Cat. #IT-14)
Transplant of GABAergic precursors restores hippocampal inhibitory function in a mouse model of seizure susceptibility.
Zipancic I, Calcagnotto ME, Piquer-Gil M, Mello LE, Alvarez-Dolado M (2010) Transplant of GABAergic precursors restores hippocampal inhibitory function in a mouse model of seizure susceptibility. Cell Transplant 19(5):549-564. doi: 10.3727/096368910X491383
Summary: Although medial ganglionic eminence-derived cells can be grafted into the neonatal brain and become functionally mature GABAergic neurons it is not clear whether the grafted cells can rescue loss of function. The authors injected mice with 1.6-2.0 ng of SSP-SAP (Cat. #IT-11) into the anterior and posterior hippocampus to eliminate GABAergic interneurons. Neuron function in mice receiving the grafts returned to near normal.
Related Products: SSP-SAP (Cat. #IT-11)
Recent progress in research on ribosome inactivating proteins.
Ng TB, Wong JH, Wang H (2010) Recent progress in research on ribosome inactivating proteins. Curr Protein Pept Sci 11(1):37-53. doi: 10.2174/138920310790274662
Summary: This review discusses recent literature on ribosome inactivating proteins including the use of saporin-based conjugates in neuroscience and cancer research. Brief descriptions of research done using 192-IgG-SAP (Cat. #IT-01), OX7-SAP (Cat. #IT-02), dermorphin-SAP (Cat. #IT-12), anti-SERT-SAP (Cat. #IT-23), SSP-SAP (Cat. #IT-11), anti-DBH-SAP (Cat. #IT-03), CTB-SAP (Cat. #IT-14), and other conjugates are provided along with basic information about ribosome inactivating proteins.
Related Products: 192-IgG-SAP (Cat. #IT-01), OX7-SAP (Cat. #IT-02), Dermorphin-SAP / MOR-SAP (Cat. #IT-12), Anti-SERT-SAP (Cat. #IT-23), SSP-SAP (Cat. #IT-11), Anti-DBH-SAP (Cat. #IT-03), CTB-SAP (Cat. #IT-14)
The cerebellum harbors a circadian oscillator involved in food anticipation.
Mendoza J, Pevet P, Felder-Schmittbuhl MP, Bailly Y, Challet E (2010) The cerebellum harbors a circadian oscillator involved in food anticipation. J Neurosci 30:1894-1904. doi: 10.1523/JNEUROSCI.5855-09.2010
Summary: The authors report on a circadian oscillator in the cerebellum that is sensitive to feeding cues. Mice received intracerebroventricular injections of 0.12, 0.25, or 0.50 µg of OX7-SAP (Cat. #IT-02). Lesioned animals displayed attenuated food-anticipatory activity, and less locomotor activity after fasting, indicating that Purkinje cells in the cerebellum are involved in the circadian connection to feeding.
Related Products: OX7-SAP (Cat. #IT-02)
Enrichment of xenograft-competent genetically modified pig cells using a targeted toxin, isolectin BS-I-B4 conjugate.
Akasaka E, Watanabe S, Himaki T, Ohtsuka M, Yoshida M, Miyoshi K, Sato M (2010) Enrichment of xenograft-competent genetically modified pig cells using a targeted toxin, isolectin BS-I-B4 conjugate. Xenotransplantation 17:81-89. doi: 10.1111/j.1399-3089.2010.00568.x
Summary: Genetically modified pigs lacking the gala1-3gal epitope may be suitable for production of organs that could be transplanted to humans. The ability to select for a homozygous population of donor somatic cells would accelerate the process of generating these animals, which would otherwise take approximately 2 years. The authors incubated a heterozygous population of 107 porcine embryonic fibroblasts with 1.6 µg of IB4-SAP (Cat. #IT-10). Even after 6 months the treated cells were negative for the agal epitope.
Related Products: IB4-SAP (Cat. #IT-10)