antibodies

Braun JL, Geromella MS, Hamstra SI, Messner HN, Fajardo VA (2021) Characterizing SERCA function in murine skeletal muscles after 35-37 days of spaceflight. Int J Mol Sci 22(21):11764. doi: 10.3390/ijms222111764 PMID: 34769190

Objective: To investigate SERCA function, SERCA regulatory protein content, and reactive oxygen/nitrogen species (RONS) protein adduction in murine skeletal muscle after 35–37 days of spaceflight.

Summary: Spaceflight affects SERCA function differently between the soleus and tibialis anterior.

Usage: Western blot (20 mg)

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Kovler ML, Gonzalez Salazar AJ, Fulton WB, Lu P, Yamaguchi Y, Zhou Q, Sampah M, Ishiyama A, Prindle T Jr, Wang S, Jia H, Wipf P, Sodhi CP, Hackam DJ (2021) Toll-like receptor 4-mediated enteric glia loss is critical for the development of necrotizing enterocolitis. Sci Transl Med 13(612):eabg3459. doi: 10.1126/scitranslmed.abg3459 PMID: 34550727

Objective: Hypothesized that enteric glia loss in the premature intestine could lead to dysmotility, exaggerated Toll-like receptor 4 (TLR4) signaling and necrotizing enterocolitis (NEC) development.

Summary: TLR4-induced enteric glia loss leads to experimental NEC through Brain Derived Neurotrophic Factor (BDNF) loss, which can be treated with compound ‘J11.’

Usage: Immunofluorescence

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Bhat SA, Fatima Z, Sood A, Shukla R, Hanif K (2021) The protective effects of AT2R agonist, CGP42112A, against angiotensin ii-induced oxidative stress and inflammatory response in astrocytes: role of AT2R/PP2A/NFκB/ROS signaling. Neurotox Res 39(6):1991-2006. doi: 10.1007/s12640-021-00403-4 PMID: 34529240

Objective: To evaluate the role and molecular mechanism of AT2R agonist CGP against Angiotensin II-induced astrocytic activation in primary astrocytes, and in a rat model of hypertension.

Summary: AT2R activation by CGP abrogated Ang II-induced astrocytic activation, by mitigating the ROS production, mitochondrial dysfunction, IκB-α degradation, NFκB nuclear translocation, and release of TNF-α in astrocytes.

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Tadokoro T, Tanaka K, Osakabe S, Kato M, Kobayashi H, Hogan BLM, Taniguchi H (2021) Dorso-ventral heterogeneity in tracheal basal stem cells. Biol Open 10(9):bio058676. doi: 10.1242/bio.058676 PMID: 34396394

Objective: To determine whether tracheal basal cells (BCs) from the dorso-ventral airways have intrinsic molecular and behavioural differences relevant to their in vivo function.

Summary: This study revealed the differences in the characteristics of BCs based on spatial distribution, and also indicated the role of epithelial-mesenchymal interactions in tissue homoeostasis in the trachea. The findings of this study suggest that careful observation is necessary in future research, as the influence of environment on the phenomenon should be considered.

Usage: Immunohistochemistry (1:100)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

de Sanctis JB (2022) Detection of nitric oxide enzymes and its metabolites. (eds. K Agrawal, J Bouchal, V Das, J Drábek, P Dzubák, M Hajdúch, K Koberna, A Ligasová, M Mistrik, JB de Sanctis, J Srovnal). In: Laboratory Techniques in Cellular and Molecular Medicine 243-252. Palacky University. PMID: 0

Objective: To provide a protocol for detection of nitrotyrosine by ELISA.

Summary: Reiss reaction sulfanilic acid (SA) in the presence of acid medium (phosphoric acid) from the diazonium salt interacts with the azo dye agent, N-alpha-naphthyl-ethylenediamine (NAD), to form a pink color which is read at 540 nm.

Usage: Western blot; detection of S-nitrosocysteine

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Sangartit W, Ha KB, Lee ES, Kim HM, Kukongviriyapan U, Lee EY, Chung CH (2021) Tetrahydrocurcumin ameliorates kidney injury and high systolic blood pressure in high-fat diet-induced type 2 diabetic mice. Endocrinol Metab (Seoul) 36(4):810-822. doi: 10.3803/EnM.2021.988 PMID: 34474516

Objective: To investigate the protective effect of tetrahydrocurcumin (THU) on intrarenal RAS expression, kidney injury, and systolic blood pressure (SBP) in high-fat diet (HFD)-induced type 2 diabetic mice.

Summary: THU alleviated kidney injury in mice with HFD-induced type 2 diabetes, possibly by blunting the activation of the intrarenal RAS/nicotinamide adenine dinucleotide phosphate oxidase IV (NOX4)/monocyte chemoattractant protein 1 (MCP-1) axis and by lowering the high SBP.

Usage: Western Blot (1:1000)

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Dookwah M, Wagner SK, Ishihara M, Yu SH, Ulrichs H, Kulik MJ, Zeltner N, Dalton S, Strauss KA, Aoki K, Steet R, Tiemeyer M (2021) Neural-specific alterations in glycosphingolipid biosynthesis and cell signaling associated with two human ganglioside GM3 Synthase Deficiency variants. bioRxiv 2021.07.29.454399. doi: 10.1101/2021.07.29.454399

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Meng H, Du Z, Lu W, Wang Q, Sun X, Jiang Y, Wang Y, Li C, Tu P (2021) Baoyuan decoction (BYD) attenuates cardiac hypertrophy through ANKRD1-ERK/GATA4 pathway in heart failure after acute myocardial infarction. Phytomedicine 89:153617. doi: 10.1016/j.phymed.2021.153617 PMID: 34157504

Objective: To explore whether BYD plays a protective role against heart failure (HF) by attenuating cardiac hypertrophy (CH) via the ANKRD1-ERK/GATA4 pathway.

Summary: BYD ameliorates CH and improves HF through the ANKRD1-ERK/GATA4 pathway, providing a novel therapeutic option for the treatment of HF.

Usage: Western Blot

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Cui YH, Zhou SF, Liu Y, Wang S, Li F, Dai RP, Hu ZL, Li CQ (2021) Injection of anti-proBDNF attenuates hippocampal-dependent learning and memory dysfunction in mice with sepsis-associated encephalopathy. Front Neurosci 15:665757. doi: 10.3389/fnins.2021.665757 PMID: 34354558

Objective: To study the effects and associated mechanism of hippocampal proBDNF in a lipopolysaccharide (LPS)-induced sepsis-associated encephalopathy (SAE) mouse model.

Summary: Mice showed cognitive dysfunction after LPS injection. The expression of proBDNF and its receptor, p75NTR, was increased in the hippocampus. The levels of BDNF and its receptor, TrkB, were decreased. An intrahippocampal or intraperitoneal injection of anti-proBDNF antibody ameliorated LPS-induced cognitive dysfunction.

Usage: Western blot (1:1000)

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Kiyokawa H, Yamaoka A, Matsuoka C, Tokuhara T, Abe T, Morimoto M (2021) Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfβ-Id2 axis, for tissue regeneration. Dev Cell 56(13):1917-1929.e9. doi: 10.1016/j.devcel.2021.05.016 PMID: 34129836

Objective: To investigate the molecular mechanisms regulating how basal cells contribute to adult tissue regeneration by shifting from slow cycling to proliferating and subsequently back to slow cycling.

Summary: TGF-β-Id2 axis commonly regulates the proliferation transitions in basal cells during development and regeneration, and its fine-tuning is critical for normal regeneration while avoiding basal cell hyperplasia.

Usage: Immunostaining

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