antibodies

Rodríguez-Rodríguez P, Vieira-Rocha MS, Quintana-Villamandos B, Monedero-Cobeta I, Prachaney P, López de Pablo AL, González MDC, Morato M, Diniz C, Arribas SM (2021) Implication of RAS in postnatal cardiac remodeling, fibrosis and dysfunction induced by fetal undernutrition. Pathophysiology 28(2):273-290. doi: 10.3390/pathophysiology28020018 PMID: 35366262

Objective: To assess if alterations in renal artery stenosis (RAS) during lactation participate in cardiac dysfunction associated with fetal undernutrition.

Summary: In rats exposed to fetal undernutrition, RAS disbalance and associated cardiac remodeling during lactation may set the basis for later heart dysfunction.

Usage: Immunohistochemistry (1:50)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Delarue Q, Robac A, Massardier R, Marie JP, Guérout N (2021) Comparison of the effects of two therapeutic strategies based on olfactory ensheathing cell transplantation and repetitive magnetic stimulation after spinal cord injury in female mice. J Neurosci Res 99(7):1835-1849. doi: 10.1002/jnr.24836 PMID: 33960512

Objective: To compare two therapeutic approaches; individual primary olfactory ensheathing cell (OEC) transplantation was compared to repetitive trans-spinal magnetic stimulation (rTSMS). Then, these two therapeutic approaches were combined for tissue repair and functional recovery after spinal cord injury (SCI).

Summary: Results indicate that primary bulbar OEC transplantation and rTSMS treatment act through different mechanisms after SCI to induce functional recovery. The combination of the two therapies does not induce an additional benefit.

Usage: Flow cytometry

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Gonzalez EA, Leitão SAT, Dos Santos Soares D, Tavares AMV, Giugliani R, Baldo G, Matte U (2021) Cardiac pathology in Mucopolysaccharidosis I mice: Losartan modifies ERK1/2 activation during cardiac remodeling. J Inherit Metab Dis 44(3):740-750. doi: 10.1002/jimd.12327 PMID: 33145772

Objective: To investigate whether the pathways influenced by losartan and propranolol may modulate the cardiac remodeling process in MPS I mice.

Summary: Losartan and propranolol restore the heart function and could be used to ameliorate the cardiac disease in MPS I.

Usage: Western Blot 1:400

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Hou G, Jiang Y, Zheng Y, Zhao M, Chen Y, Ren Y, Wang C, Li W (2021) Mechanism of Radix Astragali and Radix Salviae Miltiorrhizae ameliorates hypertensive renal damage. Biomed Res Int eCollection:5598351. doi: 10.1155/2021/5598351 PMID: 33969119

Objective: To explore the mechanism of combination of Radix Astragali (RA) and Radix Salviae Miltiorrhizae (RS) ameliorating HRD by regulation of the renal sympathetic nerve.

Summary: The combination of RA and RS may inhibit the hyperexcitability of sympathetic nerves, reduce Ang II overexpression, avoid abnormal activation of the RAS system, and improve rat renal blood flow resistance, accordingly maintaining the normal physiological structure and function of kidney tissue and having a protective effect on the cardiovascular system.

Usage: Immunohistochemistry (1:150)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Phipps BL, Suwannasual U, Lucero J, Mitchell NA, Lund AK (2021) Vehicle emissions-exposure alters expression of systemic and tissue-specific components of the renin-angiotensin system and promotes outcomes associated with cardiovascular disease and obesity in wild-type C57BL/6 male mice. Toxicol Rep 8:846-862. doi: 10.1016/j.toxrep.2021.04.001 PMID: 33948438

Objective: To investigate the hypothesis that exposure to engine emissions increases systemic and local adipocyte RAS signaling, promoting the expression of factors involved in cardiovascular disease and obesity.

Summary: Subchronic traffic-generated air pollution exposure (MVE) results in elevated expression of AT1 in the systemic vasculature, which is associated with increased vascular adhesion molecule expression, monocyte/macrophage sequestration, and production of ROS, all of which are early tissue-level signaling events in the pathogenesis of CVD. Furthermore, kidney levels of renin and Ang II receptors, AT1 and AT2, were also elevated with MVE exposure.

Usage: Immunofluorescence (1:1000)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Fang J, Sia J, Soto J, Wang P, Li LK, Hsueh YY, Sun R, Faull KF, Tidball JG, Li S (2021) Skeletal muscle regeneration via the chemical induction and expansion of myogenic stem cells in situ or in vitro. Nat Biomed Eng 5(8):864-879. doi: 10.1038/s41551-021-00696-y PMID: 33737730

Objective: To show that myogenic stem cells expanded from obtainable dermal fibroblasts or skeletal muscle stem cells lead to functional muscle regeneration when transplanted into muscle injuries in adult, aged or dystrophic mice.

Summary: A cocktail of chemicals can selectively and efficiently expand myogenic cells from dermal fibroblast-like cells and skeletal muscle stromal cells. Also, nanoparticle-delivery of the chemical cocktail induces a robust in situ activation and expansion of satellite cells for adult and aged muscle regeneration.

Usage: Immunofluorescence staining (1:100)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Brodie-Kommit J, Clark BS, Shi Q, Shiau F, Kim DW, Langel J, Sheely C, Ruzycki PA, Fries M, Javed A, Cayouette M, Schmidt T, Badea T, Glaser T, Zhao H, Singer J, Blackshaw S, Hattar S (2021) Atoh7-independent specification of retinal ganglion cell identity. Sci Adv 7(11):eabe4983. doi: 10.1126/sciadv.abe4983 PMID: 33712461

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Ebadi R, Rabiee F, Kordi-Tamandani D, Nasr-Esfahani MH, Ghaedi K (2021) Fndc5 knockdown significantly decreased the expression of neurotrophins and their respective receptors during neural differentiation of mouse embryonic stem cells. Hum Cell 34(3):847-861. doi: 10.1007/s13577-021-00517-z PMID: 33683654

Objective: To evaluate whether knockdown of Fibronectin type III domain-containing-5 (Fndc5) decreases neural differentiation of mouse embryonic stem cells (mESCs) by affecting the neurotrophins and their receptor expression.

Summary: Results suggest that decreased efficiency of neural differentiation following the reduction of Fndc5 expression could be attributed to decreased levels of NGF and BDNF proteins in addition to their cognate receptors.

Usage: Western blot (1:4000)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Dadashkhan S, Irani S, Bonakdar S, Ghalandari B (2021) P75 and S100 gene expression induced by cell-imprinted substrate and beta-carotene to nerve tissue engineering. Journal of Applied Polymer Science 138(26):50624. doi: 10.1002/app.50624

Objective: Aim to differentiate adipose-derived stem cells (ADSCs) to Schwann cells (SCs), as one of the major cell sources in nerve regeneration.

Summary: Use of synergistic application of imprinting method and beta-carotene BC. Schwann cell imprinted substrates can mimic the morphology and topography of SCs and induce differentiation signals in mesenchymal stem cells. BC stimulates neural differentiation.

Usage: Immunocytochemistry

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, Huang H, Fu W, Liang J, Wu W, Li B, Yao H, Hu H, Song E (2021) The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis. Nat Commun 12(1):1341. doi: 10.1038/s41467-021-21535-3 PMID: 33637716

Objective: To investigate the contributions of HIF-1 in regulating glycolysis of cancer cells under hypoxia

Summary: HIF Antisense LncRNA (HIFAL) has a critical regulatory role in HIF-1-driven transactivation and glycolysis, identifying HIFAL as a therapeutic target of cancer treatment.

Usage: Western blot (1:500)

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)