antibodies

Serapiglia V, Stephens CA, Joshi R, Aydin E, Oria M, Marotta M, Peiro JL, Varisco BM (2022) Fetal tracheal occlusion increases lung basal cells via increased yap signaling. Front Pediatr 9:780166. doi: 10.3389/fped.2021.780166 PMID: 35280447

Objective: To test if lung epithelial cell populations alter after tracheal occlusion (TO) with transcriptomic signatures implicating basal cells.

Summary: TO increases lung epithelial cell nuclear Yap, leading to basal cell expansion.

Usage: Immunohistochemistry (1:1000)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Cleverdon REG, Whitley KC, Marko DM, Hamstra SI, Braun JL, Roy BD, MacPherson REK, Fajardo VA (2021) Sarcoplasmic reticulum Ca2+ handling in young DBA/2J vs C57BL/10 mdx models of Duchenne muscular dystrophy. bioRxiv 2021.10.25.465805. doi: 10.1101/2021.10.25.465805

Objective: To investigate whether sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) function would differ in muscles from young D2 and C57 mdx mice.

Summary: The study demonstrates that SERCA function is drastically impaired in young D2 mdx mice.

Usage: Western blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Lin HJ, Mahendran R, Huang HY, Chiu PL, Chang YM, Day CH, Chen RJ, Padma VV, Liang-Yo Y, Kuo WW, Huang CY (2021) Aqueous extract of Solanum nigrum attenuates Angiotensin-II induced cardiac hypertrophy and improves cardiac function by repressing protein kinase C-ζ to restore HSF2 deSUMOlyation and Mel-18-IGF-IIR signaling suppression. J Ethnopharmacol 8:114728. doi: 10.1016/j.jep.2021.114728 PMID: 34634367

Objective: To investigate the ability of Solanum nigrum to attenuate Angiotensin II – induced cardiac hypertrophy and improve cardiac function through the suppression of protein kinase PKC-ζ and Mel-18-IGF-IIR signaling leading to the restoration of HSF2 desumolyation.

Summary: The data reveals the cardioprotective effect of S. nigrum inhibiting PKC-ζ with alleviated IGF IIR level in the heart that profoundly remits cardiac hypertrophy for hypertension-induced heart failure.

Usage: Western Blot

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Amano M, Amiya N, Fukushima K, Hagio H, Yamamoto N, Sakakura Y (2022) Effects of crowding stress on the hypothalamo-pituitary-interrenal axis of the self-fertilizing fish, Kryptolebias marmoratus. Comp Biochem Physiol A Mol Integr Physiol 264:111110. doi: 10.1016/j.cbpa.2021.111110 PMID: 34737084

Objective: To test whether crowding stress affects the hypothalamo-pituitary-interrenal (HPI) axis of the self-fertilizing fish, Kryptolebias marmoratus.

Summary: The number of CRH-ir cell bodies and cortisol levels were significantly higher in paired fish. The results indicate that crowding stress affects the HPI axis in K. marmoratus which thrive in small burrows with limited water volume.

Usage: Immunohistochemistry (1:2000)

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Napoli M, Wu SJ, Gore BL, Abbas HA, Lee K, Checker R, Dhar S, Rajapakshe K, Tan AC, Lee MG, Coarfa C, Flores ER (2022) ΔNp63 regulates a common landscape of enhancer associated genes in non-small cell lung cancer. Nat Commun 13(1):614. doi: 10.1038/s41467-022-28202-1 PMID: 35105868

Objective: To investigate the underlying mechanistic role regulated by ΔNp63 in lung cancer development.

Summary: Use of a ΔNp63-specific conditional knockout mouse model and xenograft models of lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC). Results show that ΔNp63 promotes non-small cell lung cancer by maintaining the lung stem cells necessary for lung cancer cell initiation and progression in quiescence. ΔNp63 regulates enhancers of cell identity genes.

Usage: Immunofluorescence and IHC (1:100)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Schulze J, Staecker H, Wedekind D, Lenarz T, Warnecke A (2022) Expression pattern of brain-derived neurotrophic factor and its associated receptors: Implications for exogenous neurotrophin application. Hear Res 413:108098. doi: 10.1016/j.heares.2020.108098 PMID: 33143996

Objective: To investigate the mechanisms of brain-derived neurotrophic factor (BDNF) in the inner ear, by analyzing the expression of mature BDNF (mBDNF), its proform proBDNF and their respective receptors the low affinity p75 neurotrophin receptor (p75NTR) and the neurotrophic receptor tyrosine kinase 2 (NTRK2).

Summary: Treatment with proBDNF is not toxic for spiral ganglion neuron (SGN) survival but simultaneously not protective. However, combined treatment of mBDNF and proBDNF maintained and perhaps slightly increased the protective effect of mBDNF.

Usage: Immunohistochemistry (1:250); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Joshi R, Batie MR, Fan Q, Varisco BM (2022) Mouse lung organoid responses to reduced, increased, and cyclic stretch. Am J Physiol Lung Cell Mol Physiol 322(1):L162-L173. doi: 10.1152/ajplung.00310.2020 PMID: 34851724

Objective: To test two mouse lung organoid mechanotransductive models to address deficiencies in cell culture models.

Summary: Cyclic stretch increased TGF-β and integrin-mediated signaling, with upstream analysis indicating roles for histone deacetylases, microRNAs, and long noncoding RNAs.

Usage: Immunostaining

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Li Y, Hollis E (2021) Basal forebrain cholinergic neurons selectively drive coordinated motor learning in mice. J Neurosci 41(49):10148-10160. doi: 10.1523/JNEUROSCI.1152-21.2021 PMID: 34750228

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP), mu p75-SAP (Cat. #IT-16)

Kim HS, Kim HY (2022) Hypertensive effects of transforming growth factor-β1 in vascular smooth muscles cells from spontaneously hypertensive rats are mediated by sulfatase 2. Cytokine 150:155754. doi: 10.1016/j.cyto.2021.155754 PMID: 34808537

Objective: To examine the role of extracellular sulfatases on TGF-β1-induced effects associated with the expression of mediators related to hypertension in vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats (SHR).

Summary: The hypertensive effects of TGF-β1 through the AT1 R pathway were mainly dependent on Sulf2 activity in SHR VSMCs. Taken together, these results suggest that Sulf2, but not Sulf1, plays a major role in mediating the increased effects of TGF-β1 in hypertensive VSMCs.

Usage: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Sasaki N, Gusain P, Hayano M, Sugaya T, Tonegawa N, Hatanaka Y, Tamura R, Okuyama K, Osada H, Ban N, Mitsukura Y, Lang RA, Mimura M, Tsubota K (2021) Violet light modulates the central nervous system to regulate memory and mood. bioRxiv 2021.11.02.466604. doi: 10.1101/2021.11.02.466604

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)