antibodies

Q: Regarding your HRP-labeled Antibody to p53 (Cat. #AB-236), your data sheet states the antigen was 15-40 a.a. Does this a.a. count from N-terminus?

A: There was an error on the data sheet which has since been corrected. The AB-236 immunogen is a KLH-conjugated peptide corresponding to amino acids 6-45. The numbering does start from the N-terminus.

Related: HRP-labeled Antibody to p53 (Cat. #AB-236)

Q: Could you please tell me if the Somatostatin 14 antibody (Cat. #AB-04) will also pick up the Somatostatin 28 residue?

A: Yes, it will, because they share the sequence of SS14. However, the Somatostatin-28 antibody (Cat. #AB-05) will not see Somatostatin-14.

Related: Anti-Somatostatin-14 (Cat. #AB-04), Anti-Somatostatin-28 (Cat. #AB-05)

Anderberg C, Li H, Fredriksson L, Andrae J, Betsholtz C, Li X, Eriksson U, Pietras K (2009) Paracrine signaling by platelet-derived growth factor-CC promotes tumor growth by recruitment of cancer-associated fibroblasts. Cancer Res 69(1):369-378. doi: 10.1158/0008-5472.CAN-08-2724 PMID: 19118022

Usage: Receptor stimulation and immunoprecipitation.  B16/mock, B16/PDGF-C, and porcine aortic endothelial cells stably expressing PDGFR-a (a-PAE) were stimulated with 100 ng/mL core PDGF-CC for 1 h on ice. Immunoprecipitation was done with an antibody to fibroblast growth factor-2.  Western blot analysis was done with 1 ug/mL antibody.

Related Products: Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)

Göz D, Studholme K, Lappi DA, Rollag MD, Provencio I, Morin LP (2008) Targeted destruction of photosensitive retinal ganglion cells with a saporin conjugate alters the effects of light on mouse circadian rhythms. PLoS ONE 3(9):e3153. doi: 10.1371/journal.pone.0003153 PMID: 18773079

Summary: Retinal ganglion cells expressing melanopsin photopigment are thought to be involved in non-image forming visual responses to light. The authors had a custom conjugate made between saporin and an anti-melanopsin antibody. A 400-ng injection of the melanopsin-SAP conjugate into the eye of a mouse resulted in a 57% loss of the targeted cells. Rabbit IgG-SAP (Cat. #IT-35) was used as a control. The data indicates that melanopsin-containing cells are involved in the response to certain non-image forming visual input.

Related Products: Melanopsin-SAP (Cat. #IT-44), Melanopsin Rabbit Polyclonal (Cat. #AB-N38), Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39), Rabbit IgG-SAP (Cat. #IT-35)

Iwatsuki K, Yoshimine T, Kishima H, Aoki M, Yoshimura K, Ishihara M, Ohnishi Y, Lima C (2008) Transplantation of olfactory mucosa following spinal cord injury promotes recovery in rats. Neuroreport 19(13):1249-1252. doi: 10.1097/WNR.0b013e328305b70b PMID: 18695502

Related Products: NGFr (192-IgG, p75) Mouse Monoclonal (Cat. #AB-N43)

Watanabe T, Ito T, Inoue G, Ohtori S, Kitajo K, Doya H, Takahashi K, Yamashita T (2008) The p75 receptor is associated with inflammatory thermal hypersensitivity. J Neurosci Res 86:3566-3574. doi: 10.1002/jnr.21808 PMID: 18709654

Summary: Inflammation, which can lead to pain hypersensitivity, not only elevates NGF levels, but also can increase the sensitivity of high-threshold receptors. NGF is thought to be a major contributor to heightened pain states due to inflammation, and in this work the authors investigated the role that the p75 neurotrophin receptor might play in NGF-induced hyperalgesia. 30 minutes prior to injection with Freund’s adjuvant, mice received 10 μl of polyclonal anti-p75 (Cat. #AB-N01) into the plantar surface of the paw. The antibody administration blocked hyperalgesia, indicating that p75 plays an important role in inflammation-induced hyperalgesia.

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Belton RJ Jr (2008) Basigin-2 is a cell surface receptor for soluble basigin ligand. J Biol Chem 283(26):17805-17814. doi: 10.1074/jbc.M801876200 PMID: 18434307

Related Products: Antibody to Basigin (Cat. #AB-42)

Kublaoui BM, Gemelli T, Tolson KP, Wang Y, Zinn AR (2008) Oxytocin deficiency mediates hyperphagic obesity of Sim1 haploinsufficient mice. Mol Endocrinol 22(7):1723-1734. doi: 10.1210/me.2008-0067 PMID: 18451093

Summary: Central administration of neuropeptides in the paraventricular nucleus (PVN) is known to inhibit feeding. Here the authors examined altered hypothalamic expression of PVN neuropeptides in Sim1+/- mice. Hypothalamic expression of several neuropeptides, including corticotrophin releasing hormone (Crh) was measured. To do so, anti-Crh (Cat. #AB-02, 1:800) was used in immunohistochemistry. The data suggests that these neurons are involved in melanocortin feeding circuits.

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)

Chidlow G, Wood JP, Manavis J, Osborne NN, Casson RJ (2008) Expression of osteopontin in the rat retina: effects of excitotoxic and ischemic injuries. Invest Ophthalmol Vis Sci 49(2):762-771. doi: 10.1167/iovs.07-0726 PMID: 18235026

Related Products: Antibody to OX7 (Cat. #AB-N08)

Dixit G, Mikoryak C, Hayslett T, Bhat A, Draper RK (2008) Cholera toxin up-regulates endoplasmic reticulum proteins that correlate with sensitivity to the toxin. Exp Biol Med (Maywood) 233(2):163-175. doi: 10.3181/0705-RM-132 PMID: 18222971

Related Products: Cholera Toxin subunit A Rabbit Polyclonal (Cat. #AB-43)