antibodies

Eccardt AM, Pelzel RJ, Bell TP, Fisher JS (2022) Stimulation of Akt phosphorylation and glucose transport by metalloporphyrins with peroxynitrite decomposition catalytic activity. Catalysts 12(8):849. doi: 10.3390/catal12080849 PMID: 37123089

Objective: To determine whether a catalytic action of iron porphyrin compounds would be related to their stimulation of insulin signaling and glucose uptake in C2C12 myotubes.

Summary: Findings suggest that iron porphyrin compounds with both peroxynitrite decomposition activity and peroxidase activity can stimulate insulin signaling and glucose transport in skeletal muscle cells.

Usage: Western blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Negueruela S, Morenilla C, Herrera M, Coca Y, Florez-Paz D, López-Cascales MT, Gomis A, Herrera E (2022) Perinatal correlated retinal activity is required for the wiring of visual axons in non-image forming nuclei. bioRxiv 2022.07.27.501692. doi: 10.1101/2022.07.27.501692

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Gao X, Zhang S, Gou J, Wen Y, Fan L, Zhou J, Zhou G, Xu G, Zhang Z (2022) Spike-mediated ACE2 down-regulation was involved in the pathogenesis of SARS-CoV-2 infection. J Infect 85(4):418-427. doi: 10.1016/j.jinf.2022.06.030 PMID: 35793758

Objective: To determine the role of angiotensin converting enzyme 2 (ACE2) as the receptor for SARS-CoV-2 entry, is also an important regulator of renin-angiotensin system (RAS) homeostasis, which plays an unsettled role in the pathogenesis of COVID-19.

Summary: The downregulation of ACE2 potentially links COVID-19 to the imbalance of RAS.

Usage: Western blot

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Li S, Li W, Yuan J, Bullova P, Wu J, Zhang X, Liu Y, Plescher M, Rodriguez J, Bedoya-Reina OC, Jannig PR, Valente-Silva P, Yu M, Henriksson MA, Zubarev RA, Smed-Sörensen A, Suzuki CK, Ruas JL, Holmberg J, Larsson C, Christofer Juhlin C, von Kriegsheim A, Cao Y, Schlisio S (2022) Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome. Nat Metab 4(6):739-758. doi: 10.1038/s42255-022-00593-x PMID: 35760869

Objective: To investigate how mitochondria sense oxygen levels.

Summary: The authors report an oxygen-sensitive regulation of TFAM, an activator of mitochondrial transcription and replication, whose alteration is linked to tumours arising in the von Hippel–Lindau syndrome. The data obtained from this study offer pharmacological avenues to sensitize therapy-resistant VHL tumours by focusing on the mitochondria.

Usage: Immunoprecipitation (IP), Immunoblot (IB); 1:1000

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Copeland EN (2022) Characterizing SERCA function in the hippocampal and prefrontal cortex regions of the brain from C57 and D2 mdx mice. Brock University St. Catharines, Ontario, Canada Thesis. PMID: 0

Objective: To investigate the differences between two models of Duchenne muscular dystrophy (DMD): D2 mouse model with young mice showing cognitive deficits, and C57, the traditional mouse model.

Summary: Impaired sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) function in both C57 and D2 mdx brain – perhaps due to heightened oxidative/nitrosative stress.

Usage: Western blot; NO-L-Cysteine Mouse Monoclonal, Conjugated as a marker of oxidative/nitrosative stress.

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Anacker A, Esser KH, Lenarz T, Paasche G (2022) Purification of fibroblasts from the spiral ganglion. Front Neurol 13:877342. doi: 10.3389/fneur.2022.877342 PMID: 35493807

Objective: To provide a purified preparation of cochlear fibroblasts, which can be used in vitro for further investigations.

Summary: Subcultivation of the primary spiral ganglion cells culture removed the neurons from the culture and increased the fibroblast to glial cell ratio in the preparations. Direct immunolabeling for the Thy1-glycoprotein and the p75NGFR-enabled fluorescence-based cell sorting. This resulted in a cell culture of cochlear fibroblasts with a purity of more than 99%.

Usage: Cell Sorting (1:400)

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Lee DS, Kim JE (2022) P2X7 receptor augments LPS-induced nitrosative stress by regulating Nrf2 and GSH levels in the mouse hippocampus. Antioxidants (Basel) 11(4):778. doi: 10.3390/antiox11040778 PMID: 35453462

Objective: To elucidate whether P2X7R affects nuclear factor-erythroid 2-related factor 2 (Nrf2) activity/expression and glutathione (GSH) synthesis under nitrosative stress in response to lipopolysaccharide (LPS)-induced neuroinflammation.

Summary: The findings indicate that P2X7R may augment LPS-induced neuroinflammation by leading to Nrf2 degradation, aberrant glutamate-glutamine cycle, and impaired cystine/cysteine uptake, which would inhibit GSH biosynthesis.

Usage: Immunohistochemistry (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Sabbah S, Papendorp C, Behrendt I, Rasras H, Cann J, Leyrer ML, Koplas E, Beltoja M, Etebari C, Gunesch AN, Carrete L, Kim MT, Manoff G, Bhatia-Lin A, Zhao T, Dowling H, Briggman KL, Berson DM (2022) Intrinsically photosensitive retinal ganglion cells evade temporal filtering to encode environmental light intensity. bioRxiv 2022.04.09.487733. doi: 10.1101/2022.04.09.487733

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Liu G, Chen Y, Wang Y, Deng X, Xiao Q, Zhang L, Xu H, Han X, Lei A, He J, Li X, Cao Y, Zhou P, He C, Wu P, Jiang W, Tan M, Chen C, Yang Q, Lu L, Deng K, Yao Z, Zhou J (2022) Angiotensin II enhances group 2 innate lymphoid cell responses via AT1a during airway inflammation. J Exp Med 219(3):e20211001. doi: 10.1084/jem.20211001 PMID: 35044462

Objective: To identify angiotensin (Ang) II as a positive regulator of Group 2 innate lymphoid cells (ILC2s).

Summary: Ang II plays a critical role in regulating ILC2 responses and airways inflammation.

Usage: Administration of Ang II (30ug) i.n. or i.p. daily for 5 days.

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Aparicio Prieto MV, Rodríguez Gallego MV, Valdivia Palacín A, Franco Iriarte Y, Hervás Barbara G, Echevarría Orella E, Casis Saenz L (2022) Local renin angiotensin system and sperm DNA fragmentation. Asian J Androl 24(2):139-146. doi: 10.4103/aja202150 PMID: 34494558

Objective: The renin angiotensin system (RAS) appears to influence male fertility at multiple levels. The relationship between the RAS and DNA integrity was analyzed.

Summary: Fragmented DNA spermatozoa have a lower capacity to respond to bioactive RAS peptides.

Usage: Immunocytochemistry

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