antibodies

Sparks NRL, Martinez IKC, Soto CH, Zur Nieden NA-O (2018) Low osteogenic yield in human pluripotent stem cells associates with differential neural crest promoter methylation. Stem Cells 36:349-362. doi: 10.1002/stem.2746 PMID: 29193426

Objective: To compare the osteogenic potential of two human induced pluripotent stem cell lines (RIV9 and RIV4) to human H9 embryonic stem cells.

Summary: The study demonstrates that different hPSC lines, including individual hiPSC clones, could have inherently different abilities to produce functional osteoblasts potentially based on the methylation marks placed on promotor regions important for lineage decisions

Usage: immunohistochemistry

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Maxwell GK, Szunyogova E, Shorrock HK, Gillingwater TH (2018) Developmental and degenerative cardiac defects in the Taiwanese mouse model of severe spinal muscular atrophy. J Anat 232:965-978. doi: 10.1111/joa.12793 PMID: 29473159

Objective: To investigate changes occurring in the heart, predominantly at pre- and early symptomatic ages, in the Taiwanese mouse model of severe Spinal muscular atrophy (SMA).

Summary: The findings support the requirement to develop systemic therapies for SMA capable of treating non-neuromuscular pathologies.

Usage: Immunohistochemistry, Western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

May Z, Kumar R, Fuehrmann T, Tam R, Vulic K, Forero J, Lucas Osma A, Fenrich K, Assinck P, Lee M, Moulson A, Shoichet M, Tetzlaff W, Biernaskie J, Fouad K (2018) Adult skin-derived precursor Schwann cell grafts form growths in the injured spinal cord of Fischer rats. Biomed Mater 13:034101. doi: 10.1088/1748-605X/aa95f8 PMID: 29068322

Objective: To improve grafted cell survival in the injured spinal cord, which is typically low.

Summary: It is of utmost importance to define the precise culture/transplantation parameters for maintenance of normal cell function and safe and effective use of cell therapy.

Usage: Immunohistochemistry (1:500)

Related Products: NGFr (192-IgG, p75) Mouse Monoclonal (Cat. #AB-N43)

Jones I, Novikova LN, Novikov LN, Renardy M, Ullrich A, Wiberg M, Carlsson L, Kingham PJ (2018) Regenerative effects of human embryonic stem cell-derived neural crest cells for treatment of peripheral nerve injury. J Tissue Eng Regen Med 12(4):e2099-e2109. doi: 10.1002/term.2642 PMID: 29327452

Objective: To determine if differentiated neural crest cells are promising supporting cell candidates to aid in peripheral nerve repair.

Summary: In this study, neural crest cells were differentiated from human embryonic stem cells. NGFR (mouse monoclonal, 1:100) Immunocytochemistry. The specificity of NGFR antibody was validated by immunocytochemical staining of both hESCs- and MACS-enriched cells.

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Nagarkoti S, Dubey M, Awasthi D, Kumar V, Chandra T, Kumar S, Dikshit M (2018) S-Glutathionylation of p47phox sustains superoxide generation in activated neutrophils. Biochim Biophys Acta Mol Cell Res 1865(2):444-454. doi: 10.1016/j.bbamcr.2017.11.014 PMID: 29195919

Objective: To investigate the role of S-Glutathionylation of p47phox.

Summary: In Phorbol 12-myristate 13-acetate (PMA) or Nitric oxide (NO) treated neutrophils a subunit of NOX2, p47phox gets glutathionylated to sustain ROS generation along with a decrease in catalase, Grx-1 activity and change in GSH/GSSG ratio.

Usage: Western blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Zhou H, Huang J, Zhu L, Cao Y (2018) Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues. Mol Med Rep 17:5184-5192. doi: 10.3892/mmr.2018.8473 PMID: 29393490

Objective: To determine whether erythropoietin (EPO) improves post‑resuscitation myocardial dysfunction and how it affects the renin‑angiotensin system.

Summary: The present study confirmed that EPO treatment is beneficial for protecting cardiac function post‑resuscitation, and the roles of EPO in alleviating post‑resuscitation myocardial dysfunction may potentially be associated with enhanced myocardial expression of AT2R.

Usage: Western blot analysis of AT-1R (1:500) in the myocardium

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Grillo SL, Stella SL, Jr. (2018) Melanopsin retinal ganglion cells are not labeled in Thy-1YFP-16 transgenic mice. Neuroreport 29:118-122. doi: 10.1097/WNR.0000000000000918 PMID: 29251688

Objective: To determine whether mRGCs are labeled with YFP in Thy-1 YFP-16 transgenic mice. The transgenic Thy-1 YFP mouse line 16 (Thy-1 YFP-16) expresses yellow-fluorescent protein (YFP) in projection neurons, including RGCs.

Summary: The majority of mRGC somas and axons are not labeled with YFP in the transgenic Thy-1 YFP-16 mouse line; therefore, this mouse model may not suitable for research involving mRGC visual pathways.

Usage: Affinity purified rabbit polyclonal antibody raised against melanopsin (1:500) was used to label mRGCs (Retinal ganglion cells expressing melanopsin). Immunolabeled Thy-1 YFP-16 mouse retina with anti-melanopsin and found that majority (∼89%) of mRGCs are not YFP-positive despite numerous other YFP-positive RGCs in the retina.

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Liu W, Zhao Y, Zhang X, Ji J (2018) Simvastatin ameliorates cognitive impairments via inhibition of oxidative stress‑induced apoptosis of hippocampal cells through the ERK/AKT signaling pathway in a rat model of senile dementia. Mol Med Rep 17(1):1885-1892. doi: 10.3892/mmr.2017.8098 PMID: 29257256

Objective: To investigate a potential mechanism underlying simvastatin activity in hippocampal cells.

Summary: The preclinical results indicate that simvastatin is efficient in preventing memory lapse and inhibiting apoptosis of hippocampal cells via the ERK/AKT signaling pathway, which may in the future improve cognitive decline and dementia in patients.

Usage: immunohistochemistry (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H (2017) Extracellular matrix from periodontal ligament cells could induce the differentiation of induced pluripotent stem cells to periodontal ligament stem cell-like cells. Stem Cells Dev 27:100-111. doi: 10.1089/scd.2017.0077 PMID: 29160151

Usage: Immunofluorescence staining (1:200 dilution)

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Trifunovic S, Manojlovic-Stojanoski M, Nestorovic N, Ristic N, Sosic-Jurjevic B, Pendovski L, Milosevic V (2018) Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat. Acta Histochem 120:129-135. doi: 10.1016/j.acthis.2017.12.006 PMID: 29338944

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)