antibodies

Cops J, Mullens W, Verbrugge FH, Swennen Q, De Moor B (2018) Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function. Sci Rep 8:17757. doi: 10.1038/s41598-018-36189-3 PMID: 30532057

Objective: The consequences of isolated abdominal venous congestion on morphology and function of the kidneys, liver and heart were studied in a rat model.

Summary: This study provides relevant insight in the pathophysiology of abdominal congestion on organ function.

Usage: Western blot. PVDF membrane was incubated overnight at 4 °C in the presence of antibody (1/2000).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Fujii S, Yoshida S, Inagaki E, Hatou S, Tsubota K, Takahashi M, Shimmura S, Sugita S (2019) Immunological properties of neural crest cells derived from human induced pluripotent stem cells. Stem Cells Dev 28(1):28-43. doi: 10.1089/scd.2018.0058 PMID: 30251915

Objective: To assess the immunological properties of human induced pluripotent stem cells derived neural crest cells (iPSC-NCCs)

Summary: Cultured human NCCs can fully suppress T cell activation in vitro and may be used in cell-based medicine.

Usage: IHC; 1:200 dilution

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Bailey D, Catron M, Kovtun O, Macdonald R, Zhang Q, Rosenthal S (2018) Single quantum dot tracking reveals serotonin transporter diffusion dynamics are correlated with cholesterol-sensitive threonine 276 phosphorylation status in primary midbrain neurons. ACS Chem Neurosci 9:2534-2541. doi: 10.1021/acschemneuro.8b00214 PMID: 29787674

Objective: Visualizing SERT behavior at the single molecule level in endogenous systems remains a challenge. The authors utilize quantum dot (QD) single particle tracking (SPT) to capture SERT dynamics in primary rat midbrain neurons.

Summary: Results provide new insights into endogenous neuronal SERT mobility and its associations with membrane cholesterol and SERT phosphorylation status.

Usage: Live Cell QD Tracking – Cells were incubated with 5 μg/mL primary SERT antibody in warm fluorescent media at 37 °C and 5% CO2 for 6 min.

Related Products: SERT Mouse Monoclonal (Cat. #AB-N40)

Manzuoerh R, Farahpour MR, Oryan A, Sonboli A (2019) Effectiveness of topical administration of Anethum graveolens essential oil on MRSA-infected wounds. Biomed Pharmacother 109:1650-1658. doi: 10.1016/j.biopha.2018.10.117 PMID: 30551419

Usage: immunohistochemistry (1:500)

Related Products: Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)

Shibata S, Hayashi R, Okubo T, Kudo Y, Katayama T, Ishikawa Y, Toga J, Yagi E, Honma Y, Quantock AJ, Sekiguchi K, Nishida K (2018) Selective laminin-directed differentiation of human induced pluripotent stem cells into distinct ocular lineages. Cell Rep 25:1668-1679. doi: 10.1016/j.celrep.2018.10.032 PMID: 30404017

Usage: Immunostaining, flow cytometry

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Ebadi R, Kordi-Tamandani DM, Ghaedi K, Nasr-Esfahani MH (2018) Comparison of two different media for maturation rate of neural progenitor cells to neuronal and glial cells emphasizing on expression of neurotrophins and their respective receptors. 45(6):2377-2391. doi: 10.1007/s11033-018-4404-4 PMID: 30306506

Objective: To compare neural differentiation potential of two different media, NB + 5%ES-FBS + N2B27 and Ko-DMEM + 5%ES-FBS for conversion of mESC derived neural progenitors (NPs) into mature neural cells with emphasis on effect of these two media on neurotrophins and their respective receptors expression.

Summary: Results indicated that NB + 5%ES-FBS + N2B27 medium promoted neural differentiation process of mESCs and caused enhancement of neurotrophins protein expression in addition to their cognate receptors.

Usage: Western Blot (1:4000)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Mao S, Wang L, Chen P, Lan Y, Guo R, Zhang M (2018) Nanoparticle-mediated delivery of Tanshinone IIA reduces adverse cardiac remodeling following myocardial infarctions in a mice model: Role of NF-κB pathway. Artif Cells Nanomed Biotechnol 46(sup3):S707-S716. doi: 10.1080/21691401.2018.1508028 PMID: 30284484

Objective: To develop a nanoparticle-mediated drug delivery system for Tanshinone IIA.

Summary: Demonstrated a novel delivery mechanism of a therapeutic agent, tanshinone IIA-NP, which prevented IκB phosphorylation and subsequent NF-κB activation resulting in the suppression of inflammatory responses and reduction of cardiomyocytes apoptosis and fibrotic process, contributing to the mitigation of LV remodeling and improvement of cardiac functions following MI.

Usage: Immunoblotting analysis for the activation of AT-1R on an ischemic border zone 4 weeks after explosion to coronary artery ligation.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Sabbah S, Papendorp C, Koplas E, Beltoja M, Etebari C, Gunesch AN, Carrete L, Kim MT, Manoff G, Bhatia-Lin A, Zhao T, Schreck D, Dowling H, Briggman KL, Berson DM (2018) Synaptic circuits for irradiance coding by intrinsically photosensitive retinal ganglion cells. bioRxiv 442954. doi: 10.1101/442954

Objective: To explore the synaptic networks responsible for the unique capacity of intrinsically photosensitive retinal ganglion cells (ipRGCs) to encode overall light intensity. This luminance signal is crucial for circadian, pupillary and related reflexive responses light.

Summary: Most ipRGCs sample from all bipolar terminals costratifying with their dendrites, but M1 cells avoid all OFF bipolar input and accept only ectopic ribbon synapses from ON cone bipolar axonal shafts. These monad synapses are equipped with as many as a dozen ribbons and only one postsynaptic process.

Usage: Immunohistochemistry of retina – after recording, retinas were fixed and counterstained with rabbit anti-melanopsin (1:1000) to enhance the fluorescence of the GFP-based GCaMP6f indicator.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38), Metabotropic Glutamate Receptor 2 (mGluR2) Mouse Monoclonal (Cat. #AB-N32)

Liu H, Chen L, Giffen KP, Stringham ST, Li Y, Judge PD, Beisel KW, He DZZ (2018) Cell-specific transcriptome analysis shows that adult pillar and deiters’ cells express genes encoding machinery for specializations of cochlear hair cells. Front Mol Neurosci 11:356. doi: 10.3389/fnmol.2018.00356 PMID: 30327589

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Kamath SP, Chen AI (2018) Myocyte enhancer factor 2c regulates dendritic complexity and connectivity of cerebellar Purkinje cells. Mol Neurobiol 56:4102–4119. doi: 10.1007/s12035-018-1363-7 PMID: 30276662

Objective: To investigate the roles of Mef2c in cerebellar Purkinje cells during the first three weeks of postnatal development.

Summary: Mef2c haploinsufficiency is implicated in behavioral deficits related to autism, schizophrenia, and intellectual disability.  Results reveal the specific expression and functional relevance of Mef2c in developing Purkinje cells and offer insight to how disruption of the expression of Mef2c in a GABAergic neuronal subtype may lead to pathogenesis of cerebellar-associated disorders.

Usage: Immunohistochemistry – mouse anti-mGluR2 (1:1500)

Related Products: Metabotropic Glutamate Receptor 2 (mGluR2) Mouse Monoclonal (Cat. #AB-N32)