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Lack of phenotypical and morphological evidences of endothelial to hematopoietic transition in the murine embryonic head during hematopoietic stem cell emergence.
Iizuka K, Yokomizo T, Watanabe N, Tanaka Y, Osato M, Takaku T, Komatsu N (2016) Lack of phenotypical and morphological evidences of endothelial to hematopoietic transition in the murine embryonic head during hematopoietic stem cell emergence. PLoS One 11:e0156427. doi: 10.1371/journal.pone.0156427 PMID: 27227884
Summary: Hemogenic endothelial cells have been observed in several embryonic tissues, such as the dorsal aorta, the placenta and the yolk sac. Recent work also suggest that the mouse embryonic head also produces hematopoietic stem cells (HSCs)/progenitors. However, a histological basis for HSC generation in the head hasn’t been determined because the hematopoietic cluster and hemogenic endothelium have not been well characterized. The authors in this study used whole-mount immunostaining and 3D confocal reconstruction techniques to analyze both c-Kit hematopoietic cluster and Runx1 hemogenic endothelium in the whole-head vasculature. Alexa488 labeled anti-NGFr (Cat. #FL-N01AP) was used in flow cytometry. The number of c-Kit hematopoietic cells was 20-fold less in the head arteries than in the dorsal aorta. In addition, nascent hematopoietic cells, observed by a budding structure and a Runx1 hemogenic endothelium, were not observed in the head. These results suggest that head HSCs may not be or are rarely generated from the endothelium in the same manner as aortic HSCs.
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified Alexa488-labeled (Cat. #AB-N01APFLA)
Polyurethane/polylactide-blend films doped with zinc ions for the growth and expansion of human olfactory ensheathing cells (OECs) and adipose-derived mesenchymal stromal stem cells (ASCs) for regenerative medicine applications
Marycz K, Marędziak M, Grzesiak J, Szarek D, Lis A, Laska J (2016) Polyurethane/polylactide-blend films doped with zinc ions for the growth and expansion of human olfactory ensheathing cells (OECs) and adipose-derived mesenchymal stromal stem cells (ASCs) for regenerative medicine applications. Polymers (Basel 8(5):175. doi: 10.3390/polym8050175 PMID: 30979270
Objective: To show that polyurethane/polylactide blends (PU/PLDL) may be further improved by the addition of ZnO nanoparticles for the delivery of bioactive zinc oxide for cells.
Summary: The researchers showed that PU/PLDL blends doped with 0.001% of ZnO exert beneficial influence on adipose stromal stem cells and olfactory ensheathing cells in vitro.
Usage: Immunofluorescence staining to verify the p75+/GFAP+ phenotype of olfactory ensheathing cells (1:1000)
Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
The specification and maturation of nociceptive neurons from human embryonic stem cells
Boisvert EM, Engle SJ, Hallowell SE, Liu P, Wang ZW, Li XJ (2015) The specification and maturation of nociceptive neurons from human embryonic stem cells. Sci Rep 5:16821. doi: 10.1038/srep16821 PMID: 26581770
Usage: Immunocytochemistry 1:200
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)
proBDNF and p75NTR control excitability and persistent firing of cortical pyramidal neurons.
Gibon J, Buckley S, Unsain N, Kaartinen V, Séguéla P, Barker P (2015) proBDNF and p75NTR control excitability and persistent firing of cortical pyramidal neurons. J Neurosci 35:9741-9753. doi: 10.1523/JNEUROSCI.4655-14.2015 PMID: 26134656
Summary: Principal neurons in the entorhinal cortex (EC) display persistent firing (PF) during working-memory tasks. Much of the communication between the hippocampus and the neocortex passes through the EC, and the EC also receives some cholinergic input from the medial septum and diagonal band of Broca. In this work the authors investigated the role of pro-brain-derived neurotrophic factor (proBDNF) and the p75 receptor in excitability and PF in the EC. The authors propose the proBDNF/p75 system as a regulator for pyramidal neuron excitability and PF in the EC, preventing runaway activity. Some of the western blot and current-clamp data was generated using Anti-p75 (Cat. #AB-N01; no concentration information provided).
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)
Characterization of cultured multipotent zebrafish neural crest cells
Kinikoglu B, Kong Y, Liao EC (2014) Characterization of cultured multipotent zebrafish neural crest cells. Exp Biol Med (Maywood) 239(2):159-168. doi: 10.1177/1535370213513997 PMID: 24326414
Summary: This work details the isolation of neural crest cells (NCCs) from transgenic zebrafish embryos expressing GFP and flow cytometry; the authors analyzed lineage markers and differentiation of the NCCs. Anti-mu p75 (Cat. #AB-N01AP) was used in immunocytochemistry at a 1:20 dilution on fixed cells.
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
BMP9 ameliorates amyloidosis and the cholinergic defect in a mouse model of Alzheimer’s disease.
Burke RM, Norman TA, Haydar TF, Slack BE, Leeman SE, Blusztajn JK, Mellott TJ (2013) BMP9 ameliorates amyloidosis and the cholinergic defect in a mouse model of Alzheimer’s disease. Proc Natl Acad Sci U S A 110(48):19567-19572. doi: 10.1073/pnas.1319297110 PMID: 24218590
Summary: During development bone morphogenetic protein 9 (BMP9) induces the cholinergic phenotype in the basal forebrain. The authors investigated the use of BMP9 as a treatment of basal forebrain cholinergic degeneration, such as is seen in Alzheimer’s disease (AD). Transgenic mice displaying AD phenotypes and expressing GFP in cholinergic neurons received icv infusions of BMP9, and several cholinergic markers were assessed. Anti-p75NTR (Cat. #AB-N01) was used in immunoblotting at a 1:3000 dilution to measure p75 levels. The results demonstrate the protective and therapeutic activity of BMP9 on AD symptoms.
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)
Adenosine inhibits glutamatergic input to basal forebrain cholinergic neurons.
Hawryluk JM, Ferrari LL, Keating SA, Arrigoni E (2012) Adenosine inhibits glutamatergic input to basal forebrain cholinergic neurons. J Neurophysiol 107(10):2769-2781. doi: 10.1152/jn.00528.2011 PMID: 22357797
Summary: Using patch-clamp recordings from mouse brain slices the authors demonstrate that adenosine not only directly inhibits cholinergic neurons in the basal forebrain, it also reduces excitatory inputs to these neurons as well. Cy3-anti-mu p75 (Cat. #FL-05, 40-60 ng) was injected into the lateral cerebroventricle.
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified Cy3-labeled (Cat. #AB-N01APFL3)
Neurotrophin/receptor expression in urinary bladder of mice with overexpression of NGF in urothelium.
Girard BM, Malley SE, Vizzard MA (2011) Neurotrophin/receptor expression in urinary bladder of mice with overexpression of NGF in urothelium. Am J Physiol Renal Physiol 300(2):F345-55. doi: 10.1152/ajprenal.00515.2010 PMID: 21048026
Summary: Chronic overexpression of nerve growth factor (NGF) in the urinary bladder results in neuronal sprouting, increased voiding frequency, and referred somatic hypersensitivity. The authors investigated several growth factor and receptor responses to chronic overexpression of NGF. A p75 antibody (Cat. #AB-N01) was used to obtain the immunohistochemistry data. The data suggest that changes due to NGF overexpression may be a compensatory attempt to reduce voiding frequency.
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)
BMP9 (bone morphogenetic protein 9) induces NGF as an autocrine/paracrine cholinergic trophic factor in developing basal forebrain neurons.
Schnitzler AC, Mellott TJ, Lopez-Coviella I, Tallini YN, Kotlikoff MI, Follettie MT, Blusztajn JK (2010) BMP9 (bone morphogenetic protein 9) induces NGF as an autocrine/paracrine cholinergic trophic factor in developing basal forebrain neurons. J Neurosci 30(24):8221-8228. doi: 10.1523/JNEUROSCI.5611-09.2010 PMID: 20554873
Summary: Bone morphogentic protein (BMP) 9 is a cholinergic differentiation factor that increases acetylcholine synthesis and choline acetyltransferase gene expression. The authors investigated whether BMP9 could induce cholinergic trophic factors in murine septal cells. One experiment involved the sorting of E18 septal cells using anti-p75 (Cat. #AB-N01AP, 5µg/2×106 cells). The increased Ngf gene expression in response to BMP9 in p75-positive basal forebrain cholinergic neurons indicates an autocrine/paracrine role for NGF in the development and maintenance of these cells.
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Basal cells as stem cells of the mouse trachea and human airway epithelium
Rock JR, Onaitis MW, Rawlins EL, Lu Y, Clark CP, Xue Y, Randell SH, Hogan BL (2009) Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A 106(31):12771-12775. doi: 10.1073/pnas.0906850106 PMID: 19625615
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)