References

Huang L, Yuan T, Tan M, Xi Y, Hu Y, Tao Q, Zhao Z, Zheng J, Han Y, Xu F, Luo M, Sollars P, Pu M, Pickard G, So K, Ren C (2017) A retinoraphe projection regulates serotonergic activity and looming-evoked defensive behaviour. Nat Commun 8:14908. doi: 10.1038/ncomms14908 PMID: 28361990

Objective: To investigate how the dorsal raphe nucleus (DRN) and superior colliculus work in concert to extract and translate visual threats into defensive behavioural responses.

Summary: A dedicated population of RGCs signals rapidly approaching visual threats and their input to the DRN controls a serotonergic self-gating mechanism that regulates innate defensive responses.

Usage: Mice received bilateral intraocular injection (2 μg per eye) made between Streptavidin-Saporin and a biotinylated CTB antibody, or Anti-Melanopsin-SAP (2 μg per eye). For detection of melanopsin, retinas were incubated for 3 days at 4 °C with anti-melanopsin (1:600).

Related Products: Streptavidin-ZAP (Cat. #IT-27), Melanopsin-SAP (Cat. #IT-44), Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Malheiros-Lima M, Takakura A, Moreira T (2017) Depletion of rostral ventrolateral medullary catecholaminergic neurons impairs the hypoxic ventilatory response in conscious rats. Neuroscience 351:1-14.. doi: 10.1016/j.neuroscience.2017.03.031

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Kobayashi K, Maezawa T, Tanaka H, Onuki H, Horiguchi Y, Hirota H, Ishida T, Horiike K, Agata Y, Aoki M, Hoshi M, Matsumoto M (2017) The identification of ᴅ-tryptophan as a bioactive substance for postembryonic ovarian development in the planarian Dugesia ryukyuensis. Sci Rep 7:45175. doi: 10.1038/srep45175 PMID: 28338057

Usage: IHC 1:50

Ye J-H, Fu R, He W (2017) The rostromedial tegmental nucleus and alcohol addiction. Oncotarget 8:18624-18625.. doi: 10.18632/oncotarget.15822

Summary: The authors discuss their work with Dermorphin-SAP (Cat. #IT-12) and their demonstration that damage of RMTg MOR-expressing GABAergic neurons by Dermorphin-SAP increased the intake and preference for alcohol, boosted the expression and slowed down the extinction of alcohol conditioned place preference, and increased locomotion. Microinjection of DS into the RMTg substantially reduced the number of RMTg cells. Importantly, the rats that received DS injection elevated their alcohol intake and preference compared to those that received an injection of Blank-SAP (Cat. #IT-21), which did not cause neuronal damage.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12), Blank-SAP (Cat. #IT-21)

Ekman M, Zhu B, Swärd K, Uvelius B (2017) Neurite outgrowth in cultured mouse pelvic ganglia – Effects of neurotrophins and bladder tissue. Auton Neurosci 205:41-49. doi: 10.1016/j.autneu.2017.03.004 PMID: 28347639

Objective: To characterize the effects of the neurotrophins nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3) on the sprouting rate of sympathetic and parasympathetic neurites from the female mouse ganglion.

Summary: All three factors (BDNF, NT-3 and NGF) stimulated neurite outgrowth of both sympathetic and parasympathetic neurites although BDNF and NT-3 had a higher stimulatory effect on parasympathetic ganglion cells. Active forms of BDNF and NT-3 were detected in urinary bladder tissue, whereas tissue from the diaphragm expressed NGF.

Usage: in vitro culture (2 ul/ml)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Gupta A, Anjomani-Virmouni S, Koundouros N, Dimitriadi M, Choo-Wing R, Valle A, Zheng Y, Chiu YH, Agnihotri S, Zadeh G, Asara JM, Anastasiou D, Arends MJ, Cantley LC, Poulogiannis G (2017) PARK2 depletion connects energy and oxidative stress to PI3K/Akt activation via PTEN S-Nitrosylation. Mol Cell 65(6):999-101.e7. doi: 10.1016/j.molcel.2017.02.019 PMID: 28306514

Objective: To investigate the missing piece in the dynamic signaling and metabolic network governing PI3K/Akt activation.

Summary: PARK2 inactivation connects energy and oxidative stress to Akt activation via redox-mediated inactivation of PTEN by S-nitrosylation to support cell survival under conditions of energy deprivation.

Usage: Immunoblotting

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Gholamin S, Mitra SS, Feroze AH, Liu J, Kahn SA, Zhang M, Esparza R, Richard C, Ramaswamy V, Remke M, Volkmer AK, Willingham S, Ponnuswami A, McCarty A, Lovelace P, Storm TA, Schubert S, Hutter G, Narayanan C, Chu P, Raabe EH, Harsh G 4th, Taylor MD, Monje M, Cho YJ, Majeti R, Volkmer JP, Fisher PG, Grant G, Steinberg GK, Vogel H, Edwards M, Weissman IL, Cheshier SH (2017) Disrupting the CD47-SIRPα anti-phagocytic axis by a humanized anti-CD47 antibody is an efficacious treatment for malignant pediatric brain tumors. Sci Transl Med 9(381):eaaf2968. doi: 10.1126/scitranslmed.aaf2968 PMID: 28298418

Summary: A humanized anti-CD47 antibody has proven to be an effective treatment for malignant pediatric brain tumors by disrupting the CD47-SIRPα anti-phagocytic axis. This breakthrough therapy shows promise in improving outcomes for young patients with these challenging tumors.

Yu Y-Q, Barry DM, Hao Y, Liu X-T, Chen Z-F (2017) Molecular and neural basis of contagious itch behavior in mice. Science 355:1072. doi: 10.1126/science.aak9748

Summary: The authors selectively ablated the SCN gastrin-releasing peptide receptor (GRPR) neurons using Bombesin-SAP (Cat. #IT-40), a peptide-conjugated toxin that kills GRPR neurons in the spinal cord. After bilateral injection of Bombesin-SAP into the SCN, immunohistochemistry showed that Bombesin-SAP injection resulted in ablation of SCN GRPR+ neurons.

Related Products: Bombesin-SAP (Cat. #IT-40)

Maurice T, Goguadze N (2017) Sigma-1 (σ1) receptor in memory and neurodegenerative diseases. Handb Exp Pharmacol 244:81-108. doi: 10.1007/164_2017_15

Related Products: 192-IgG-SAP (Cat. #IT-01)

Somasundaram P, Wyrick G, Fernandez D, Ghahari A, Pinhal C, Simmonds Richardson M, Rupp A, Cui L, Wu Z, Brown R, Badea T, Hattar S, Robinson P (2017) C-terminal phosphorylation regulates the kinetics of a subset of melanopsin-mediated behaviors in mice. Proc Natl Acad Sci U S A 114:2741-2746. doi: 10.1073/pnas.1611893114 PMID: 28223508

Summary: The authors show that the melanopsin photoresponse shutoff due to C-terminal phosphorylation determines the kinetics of the intrinsic light response in ipRGCs, the PLR, and reentrainment, but not masking and phase angle of entrainment. Immunofluorescence was performed using rabbit Anti-Melanopsin (1:1,000, Cat. #AB-N38) as the primary antibody with a 2-d incubation period, followed by goat anti-rabbit IgG 488 as the secondary antibody.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)