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Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues
Zhou H, Huang J, Zhu L, Cao Y (2018) Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues. Mol Med Rep 17:5184-5192. doi: 10.3892/mmr.2018.8473 PMID: 29393490
Objective: To determine whether erythropoietin (EPO) improves post‑resuscitation myocardial dysfunction and how it affects the renin‑angiotensin system.
Summary: The present study confirmed that EPO treatment is beneficial for protecting cardiac function post‑resuscitation, and the roles of EPO in alleviating post‑resuscitation myocardial dysfunction may potentially be associated with enhanced myocardial expression of AT2R.
Usage: Western blot analysis of AT-1R (1:500) in the myocardium
Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)
The role of the supramammillary area of the hypothalamus in cognitive functions
Shim HS, Park H-J, Lee M-S, Ye M, Shim I (2018) The role of the supramammillary area of the hypothalamus in cognitive functions. Animal Cells and Systems 22:37-44. doi: 10.1080/19768354.2018.1427627
Related Products: 192-IgG-SAP (Cat. #IT-01)
Melanopsin retinal ganglion cells are not labeled in Thy-1YFP-16 transgenic mice
Grillo SL, Stella SL, Jr. (2018) Melanopsin retinal ganglion cells are not labeled in Thy-1YFP-16 transgenic mice. Neuroreport 29:118-122. doi: 10.1097/WNR.0000000000000918 PMID: 29251688
Objective: To determine whether mRGCs are labeled with YFP in Thy-1 YFP-16 transgenic mice. The transgenic Thy-1 YFP mouse line 16 (Thy-1 YFP-16) expresses yellow-fluorescent protein (YFP) in projection neurons, including RGCs.
Summary: The majority of mRGC somas and axons are not labeled with YFP in the transgenic Thy-1 YFP-16 mouse line; therefore, this mouse model may not suitable for research involving mRGC visual pathways.
Usage: Affinity purified rabbit polyclonal antibody raised against melanopsin (1:500) was used to label mRGCs (Retinal ganglion cells expressing melanopsin). Immunolabeled Thy-1 YFP-16 mouse retina with anti-melanopsin and found that majority (∼89%) of mRGCs are not YFP-positive despite numerous other YFP-positive RGCs in the retina.
Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)
Intracerebroventricular administration of 192IgG-saporin alters expression of microglia-associated genes in the dorsal but not ventral hippocampus
Dobryakova YV, Kasianov A, Zaichenko MI, Stepanichev MY, Chesnokova EA, Kolosov PM, Markevich VA, Bolshakov AP (2018) Intracerebroventricular administration of 192IgG-saporin alters expression of microglia-associated genes in the dorsal but not ventral hippocampus. Front Mol Neurosci 10:429. doi: 10.3389/fnmol.2017.00429
Objective: To analyze the postponed consequences of cholinergic deficit in different parts of the hippocampus.
Summary: Disturbance of memory-associated behavior after administration of 192-IgG-SAP is associated with upregulation of microglia-associated genes in the dorsal but not ventral hippocampus.
Usage: Rats received bilateral i.c.v.infusions of 192-IgG-SAP (4 mcg/site).
Related Products: 192-IgG-SAP (Cat. #IT-01)
Simvastatin ameliorates cognitive impairments via inhibition of oxidative stress‑induced apoptosis of hippocampal cells through the ERK/AKT signaling pathway in a rat model of senile dementia
Liu W, Zhao Y, Zhang X, Ji J (2018) Simvastatin ameliorates cognitive impairments via inhibition of oxidative stress‑induced apoptosis of hippocampal cells through the ERK/AKT signaling pathway in a rat model of senile dementia. Mol Med Rep 17(1):1885-1892. doi: 10.3892/mmr.2017.8098 PMID: 29257256
Objective: To investigate a potential mechanism underlying simvastatin activity in hippocampal cells.
Summary: The preclinical results indicate that simvastatin is efficient in preventing memory lapse and inhibiting apoptosis of hippocampal cells via the ERK/AKT signaling pathway, which may in the future improve cognitive decline and dementia in patients.
Usage: immunohistochemistry (1:500)
Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Raphe pallidus is not important to central chemoreception in a rat model of Parkinson’s disease.
Oliveira LM, Moreira TS, Takakura AC (2018) Raphe pallidus is not important to central chemoreception in a rat model of Parkinson’s disease. Neuroscience 369:350-362. doi: 10.1016/j.neuroscience.2017.11.038
Objective: To investigate if serotonin-expressing neurons in the Raphe pallidus/parapyramidal region (RPa/PPy) are also involved in the modulation of breathing during central chemoreception activation in a PD animal model.
Related Products: Anti-SERT-SAP (Cat. #IT-23), Saporin (Cat. #PR-01)
Extracellular matrix from periodontal ligament cells could induce the differentiation of induced pluripotent stem cells to periodontal ligament stem cell-like cells
Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H (2017) Extracellular matrix from periodontal ligament cells could induce the differentiation of induced pluripotent stem cells to periodontal ligament stem cell-like cells. Stem Cells Dev 27:100-111. doi: 10.1089/scd.2017.0077 PMID: 29160151
Usage: Immunofluorescence staining (1:200 dilution)
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Immunolesion of melanopsin neurons causes gonadal regression in Pekin drakes (Anas platyrhynchos domesticus).
Potter H, Alenciks E, Frazier K, Porter A, Fraley GS (2018) Immunolesion of melanopsin neurons causes gonadal regression in Pekin drakes (Anas platyrhynchos domesticus). Gen Comp Endocrinol 256:16-22. doi: 10.1016/j.ygcen.2017.08.006
Objective: Examine effects of loss of melanopsin in drakes.
Summary: Loss of melanopsin in PMM elicits decrease in GnRH mRNA expression, gonadal regression, and sex behaviors in drakes.
Usage: To specifically lesion melanopsin-receptive neurons, 3 μl of an anti-melanopsin-saporin conjugate (MSAP, 100 ng/ul) was injected into the lateral ventricle (n = 10). Control drakes were injected with 3 μl of equimolar unconjugated anti-melanopsin and saporin (SAP, n = 10).
Related Products: Melanopsin-SAP (Cat. #IT-44), Saporin (Cat. #PR-01)
Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat
Trifunovic S, Manojlovic-Stojanoski M, Nestorovic N, Ristic N, Sosic-Jurjevic B, Pendovski L, Milosevic V (2018) Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat. Acta Histochem 120:129-135. doi: 10.1016/j.acthis.2017.12.006 PMID: 29338944
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
CD44 signaling mediates high molecular weight hyaluronan-induced antihyperalgesia.
Ferrari LF, Khomula EV, Araldi D, Levine JD (2018) CD44 signaling mediates high molecular weight hyaluronan-induced antihyperalgesia. J Neurosci 38(2):308-321. doi: 10.1523/JNEUROSCI.2695-17.2017
Objective: To study the role of the cognate hyaluronan receptor, CD44, signaling in anti-hyperalgesia induced by high molecular weight hyaluronan (HMWH).
Summary: These results demonstrate the central role of CD44 signaling in HMWH-induced anti-hyperalgesia, and establish it as a therapeutic target against inflammatory and neuropathic pain.
Usage: Both IB4-SAP and SSP-SAP were diluted in saline to doses previously shown to deplete nonpeptidergic (3.2 mcg/rat for IB4-SAP) and peptidergic (100 ng/rat for SSP-SAP) fibers. The toxins were administered intrathecally, in a volume of 20 mcl, 14 d before intradermal injection of LMWH on the dorsum of the hindpaw. Treatment with either conjugate, or a combination of the two, did not significantly affect mechanical nociceptive threshold.
Related Products: IB4-SAP (Cat. #IT-10), SSP-SAP (Cat. #IT-11), Anti-CD44-SAP (Cat. #IT-72)