References

Su Y, Liu Y, Behrens CR, Bidlingmaier S, Lee NK, Aggarwal R, Sherbenou DW, Burlingame AL, Hann BC, Simko JP, Premasekharan G, Paris PL, Shuman MA, Seo Y, Small EJ, Liu B (2018) Targeting CD46 for both adenocarcinoma and neuroendocrine prostate cancer. JCI Insight 3(17):e121497. doi: 10.1172/jci.insight.121497 PMID: 30185663

Objective: To investigate the suitability of a CD46 antibody as a metastatic castration-resistant prostate cancer (mCRPC) anti-tumor agent.

Summary: CD46 is an excellent candidate for antibody-based therapy development, which has potential to be applicable to both adenocarcinoma and neuroendocrine types of mCRPC that are resistant to current treatment.

Usage: Biotinylated UA20 IgG and Streptavidin-ZAP were mixed at a molar ratio of 1:1. The conjugate was used in cytotoxicity assays and shown to specifically kill the mCRPC cells.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Lénárd L, László K, Kertes E, Ollmann T, Péczely L, Kovács A, Kállai V, Zagorácz O, Gálosi R, Karádi Z (2018) Substance P and neurotensin in the limbic system: Their roles in reinforcement and memory consolidation. Neurosci Biobehav Rev 85:1-20. doi: 10.1016/j.neubiorev.2017.09.003

Summary: A specific neurotoxin, SSP-SAP can cause selective ablation of NK1 receptor expressing cells. This lesion in the BLA of rats resulted in anxiogenic effect, while in the CeA had no effect in EPM test.

See: Truitt WA et al. Anxiety-like behavior is modulated by a discrete subpopulation of interneurons in the basolateral amygdala. Neuroscience 160:284-294, 2009.

Related Products: SSP-SAP (Cat. #IT-11)

Berg D, Kartheiser K, Leyrer M, Saali A, Berson D (2018) Molecular diversity of intrinsically photosensitive ganglion cells. bioRxiv 381004. doi: 10.1101/381004

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Yang WH, Cheng CY, Chen MF, Wang TC (2018) Cell subpopulations overexpressing p75NTR have tumor-initiating properties in the c6 glioma cell line. 38(9):5183-5192. doi: 10.21873/anticanres.12841 PMID: 30194166

Objective: This study is focused on the possible role of p75NTR in glioma tumor initiation.

Summary: Cells with p75NTR overexpression demonstrated certain unique characteristics of tumor-initiating cells, such as neurosphere formation, high colony proliferation, and resistance to radio- and chemotherapy. With regard to the heterogeneous composition of glioma cells, p75NTR can be used as an alternative marker to identify a glioma subpopulation with tumor-initiating properties.

Usage: flow cytometry

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Micakovic T, Papagiannarou S, Clark E, Kuzay Y, Abramovic K, Peters J, Sticht C, Volk N, Fleming T, Nawroth P, Hammes HP, Alenina N, Grone HJ, Hoffmann SC (2018) The angiotensin II type 2 receptors protect renal tubule mitochondria in early stages of diabetes mellitus. Kidney Int 94:937-950. doi: 10.1016/j.kint.2018.06.006 PMID: 30190172

Objective: To investigate the role of the angiotensin II type 2 receptor (AT-2R) at the early stages of diabetic nephropathy.

Summary: Targeted AT-2R overexpression in renal cells may open new avenues to develop novel types of drugs preventing diabetic nephropathy.

Usage: Histology, immunohistochemistry; 1:100

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Donnelly CR, Gabreski NA, Suh EB, Chowdhury M, Pierchala BA (2018) Non-canonical Ret signaling augments p75-mediated cell death in developing sympathetic neurons. J Cell Biol 217:327. doi: 10.1083/jcb.201703120 PMID: 30018091

Summary: The authors investigated the interactions of pro-survival and pro-apoptotic receptors in Programmed Cell Death (PCD) using the sympathetic nervous system as a model. They demonstrate that Ret, a receptor tyrosine kinase required for the survival of many neuronal populations, is restricted to a subset of degenerating neurons that rapidly undergo apoptosis.

Usage: Proximity ligation assays (1:500); Western blotting (1:1,000)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Tools to Study Programmed Cell Death

Naser PV, Kuner R (2018) Molecular, cellular and circuit basis of cholinergic modulation of pain. Neuroscience 387:135-148. doi: 10.1016/j.neuroscience.2017.08.049

Related Products: 192-IgG-SAP (Cat. #IT-01)

Diaz-delCastillo M, Woldbye DPD, Heegaard AM (2018) Neuropeptide Y and its involvement in chronic pain. Neuroscience 387:162-169. doi: 10.1016/j.neuroscience.2017.08.050

Related Products: NPY-SAP (Cat. #IT-28)

Pitake S, Ralph PC, DeBrecht J, Mishra SK (2018) Atopic dermatitis linked cytokine interleukin-31 induced itch mediated via a neuropeptide natriuretic polypeptide b. Acta Derm Venereol 98:795-796. doi: 10.2340/00015555-2977

Objective: To determine if NPPB is involved as a neuropeptide in IL-31-mediated itch in atopic dermatitis (AD) via natriuretic polypeptide receptor A (NPRA) in the spinal cord.

Summary: This study reveals an important role of neuropeptide NPPB in AD that could provide a therapeutic target for alleviating chronic itch associated with AD.

Usage: To further demonstrate the IL-31-mediated itch response by NPRA receptors expressed in the spinal cord, Nppb-SAP (5 μg) was used to eliminate neurons expressing NPRA receptors in the spinal cord.

Related Products: Nppb-SAP (Cat. #IT-69)

Zhang Z, Jiang SM, Ma YP, Dai PL, Wang YN, Zou GM, Gao HM, Yang Y, Li WG (2019) Expression of the intrarenal angiotensin receptor and the role of renin-angiotensin system inhibitors in IgA nephropathy. Mol Cell Biochem 453:103-110. doi: 10.1007/s11010-018-3435-4 PMID: 30159797

Objective: To investigate the role of intrarenal renin-angiotensin system (RAS) in the development of human kidney disease. Patients with IgA nephropathy (IgAN) were screened and their clinical characteristics analyzed.

Summary: Overexpression of AT1R on the mesangial cells of IgAN patients is associated with mesangial cell proliferation, glomerular segmental sclerosis, and crescent formation.

Usage: immunohistochemistry (1:500)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)