References

Kim M, Vargas M, Harlan B, Killoy K, Ball L, Comte-Walters S, Gooz M, Yamamoto Y, Beckman J, Barbeito L, Pehar M (2018) Nitration and glycation turn mature NGF into a toxic factor for motor neurons: A role for p75. Antioxid Redox Signal 28:1587-1602. doi: 10.1089/ars.2016.6966 PMID: 28537420

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Harvey BP, Cohen-Solal J, Kaymakcalan Z (2018) SAT0058 Adalimumab: TNF complexes are cleared more efficiently by human osteoclasts than those with etanercept through FCG-receptor binding and internalisation. Ann Rheum Dis 77:893. EULAR 2018, Amsterdam, The Netherlands doi: 10.1136/annrheumdis-2018-eular.3804

Objective: To determine whether Fc-gamma receptor (FcgR)-mediated internalization of the biologic:TNF complexes is a contributing mechanism responsible for the difference in effectiveness between ADA and ETN in preventing TNF- enhanced OCgenesis.

Summary: Human osteoclast  (OC) precursors can bind and internalise ADA:TNF complexes more efficiently than ETN:TNF complexes. In addition, this process is partially mediated through FcgRII.

Usage: FcgR-mediated nternalization was assessed by monitoring a reduction in OC survival in response to preformed bio- logic: TNF complexes (25:1 ratio) bound with FabFC-ZAP human ± FcgR blocking antibodies.

Related Products: FabFc-ZAP human (Cat. #IT-65)

Sparks NR (2018) The embryotoxic effects of harm reduction tobacco products on osteoblasts developing from human embryonic stem cells. University of California Riverside Thesis.

Objective: To investigate the effect of maternal smoking in the development of embryonic skeleton and the molecular mechanisms involved in skeletal embryotoxicity induced by conventional and harm-reduction tobacco.

Summary: An in vitro model based on human embryonic stem cells was developed, that mimics the development of the human embryo; their differentiation into osteoblasts in vitro can be used to study chemical toxicity and the molecular mechanisms thereof. Tobacco-induced oxidative stress disrupts osteogenic differentiation in human embryonic stem cells.

Usage: Immunocytochemistry

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Sakamoto A, Kato K, Hasegawa T, Ikeda S (2018) An agonistic antibody to EPHA2 exhibits antitumor effects on human melanoma cells. Anticancer Res 38:3273-3282. doi: 10.21873/anticanres.12592

Objective: Investigate the therapeutic potential of antibody to EPHA2 against melanoma in vitro.

Summary: Observations indicate a promising role for EPHA2 as a target in antibody treatments for melanoma, and demonstrate the potential therapeutic effects of an agonistic antibody to EPHA2.

Usage: A375 cells were plated into a flat-bottom, 96-well plate (2,000 cells per well) and incubated for 4 days at 37˚C. Cell suspension included different concentrations of Mab-ZAP, along with either anti-EPHA2 mAb (SHM16, SHM17, or SHM20 at 2 μg/ml final concentration), or a control IgG1 mAb (2 μg/ml final concentration).

Related Products: Mab-ZAP (Cat. #IT-04)

Suarez AN, Hsu TM, Liu CM, Noble EE, Cortella AM, Nakamoto EM, Hahn JD, de Lartigue G, Kanoski SE (2018) Gut vagal sensory signaling regulates hippocampus function through multi-order pathways. Nat Commun 9(1):2181. doi: 10.1038/s41467-018-04639-1

Objective: To determine the  endogenous relevance of GIderived vagal HPC communication.

Summary: Endogenous derived vagal sensory signaling promotes HPC-dependent memory function via a multi-order brainstem–septal pathway, thereby identifying a previously unknown role for the gut–brain axis in memory control.

Usage: A 1-µl volume of CCK-SAP (250 ng/µl) or control Saporin (250 ng/µl) was injected at two sites: 0.5 µl rostral and 0.5 µl caudal to the laryngeal nerve branch.

Related Products: CCK-SAP (Cat. #IT-31), Saporin (Cat. #PR-01)

Sadhukhan R, Brown N, Ouellette D, Banach D, Filoti DI, Winarta D, Raghavendra R, Sousa S, Darcy A, Alessandri L, Ivanov A, Bose S, Eaton L, Preston G, Freeman J, Correia I (2018) Engineering elastic properties into an anti-TNFα monoclonal antibody. Cogent Biol 4(1):1469387. doi: 10.1080/23312025.2018.1469387

Objective: To engineer elastic properties into a TNFalpha antibody.

Summary: The results presented in this report with an anti-TNFα ELP mAb are a foundation for building on a new generation of fusion ELP mAbs, or other formats, that are stable, active, responsive to cues in local environment, and, with the FcRn mutation, cleared rapidly from circulation. More detailed studies are warranted to identify the appropriate ELP sequences for IA delivery, calculate residence time in the IA space, and demonstrate pharmacodynamics effect of the ELP-fusion protein.

Usage: Fab-ZAP human was mixed with anti-TNFα-ELP fusion monoclonal.

Related Products: Fab-ZAP human (Cat. #IT-51)

Sánchez-Migallón MC, Valiente-Soriano FJ, Nadal-Nicolás FM, Di Pierdomenico J, Vidal-Sanz M, Agudo-Barriuso M (2018) Survival of melanopsin expressing retinal ganglion cells long term after optic nerve trauma in mice. Exp Eye Res 174:93-97. doi: 10.1016/j.exer.2018.05.029 PMID: 29856984

Summary: Melanopsin-positive retinal ganglion cells (RGCs) do not respond to axotomy in the same way than the rest of RGCs, and so while image-forming RGCs die in two exponential phases, non-image forming RGCs die only during the first quick phase.

Usage: Retinas were dissected as flat mounts and double-immunostained against melanopsin (1:5000).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Motohashi T, Kunisada T (2019) Direct conversion of mouse embryonic fibroblasts into neural crest cells. (eds. Turksen K). In: Skin Stem Cells. Methods in Molecular Biology. 1879:307-321. Humana Press, New York, NY. doi: 10.1007/7651_2018_145 PMID: 29797008

Objective: To describe methods for the direct conversion of mouse embryonic fibroblasts into neural crest cells (NCCs).

Summary: Sox10-IRES-Venus mouse fibroblasts were used for the conversion and isolation of converted NCCs as SOX10-positive cells.

Usage: Immunostaining for Flow Cytometry

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Dhez AC, Benedetti E, Antonosante A, Panella G, Ranieri B, Florio TM, Cristiano L, Angelucci F, Giansanti F, Di Leandro L, d’Angelo M, Melone M, De Cola A, Federici L, Galzio R, Cascone I, Raineri F, Cimini A, Courty J, Giordano A, Ippoliti R (2018) Targeted therapy of human glioblastoma via delivery of a toxin through a peptide directed to cell surface nucleolin. J Cell Physiol 233(5):4091-4105. doi: 10.1002/jcp.26205 PMID: 28941284

Staib JM, Della Valle R, Knox DK (2018) Disruption of medial septum and diagonal bands of Broca cholinergic projections to the ventral hippocampus disrupt auditory fear memory. Learn Mem 152:71-79. doi: 10.1016/j.nlm.2018.05.009

Objective: To determine which efferent projections are critical for contextual fear memory discrimination and extinction memory.

Summary: The results of this study suggest that MS/vDBB cholinergic neurons are critical for fear and extinction memory.

Usage: 192-IgG saporin was infused into all brain regions at a concentration of 0.2 μg/μL dissolved in 0.2 M PBS. The total volume of each injection was 0.5 μL. Sham surgeries were accomplished using the same volume (0.5 μL) of PBS.

Related Products: 192-IgG-SAP (Cat. #IT-01)