References

Araldi D, Ferrari LF, Levine JD (2018) Role of GPCR (mu-opioid)-receptor tyrosine kinase (epidermal growth factor) crosstalk in opioid-induced hyperalgesic priming (type II). Pain 159(5):864-875. doi: 10.1097/j.pain.0000000000001155

Objective: To determine the the mechanisms mediating the induction of opioid-induced hyperalgesia and the prolongation of prostaglandinE2-induced hyperalgesia in type II hyperalgesic priming.

Summary: Understanding the mechanisms responsible for the induction of type II hyperalgesic priming, a form of neuroplasticity in the peripheral terminal of the primary afferent nociceptor, may provide useful information for the design of drugs with improved therapeutic profiles to treat neuroplasticity induced by chronic use of opioids.

Usage: SSP-SAP was prepared in saline (5 ng/mL), and 20 mL was injected intrathecally into rats, 14 days before nociceptive tests.

Related Products: SSP-SAP (Cat. #IT-11)

Tata A, Kobayashi Y, Chow RD, Tran J, Desai A, Massri AJ, McCord TJ, Gunn MD, Tata PR (2018) Myoepithelial cells of submucosal glands can function as reserve stem cells to regenerate airways after injury. Cell Stem Cell 22:668-683. doi: 10.1016/j.stem.2018.03.018 PMID: 29656943

Objective: To show that myoepithelial cells (MECs) of the submucosal glands (SMGs) proliferate and migrate to repopulate the airway surface epithelium (SE) in multiple injury models.

Summary: SMG-like cells appear on the SE of extra-and intra-lobular airways of large animal lungs following severe injury. The transcription factor SOX9 is necessary for MEC plasticity in airway regeneration.

Usage: Immunostaining

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Lynch TJ, Anderson PJ, Rotti PG, Tyler SR, Crooke AK, Choi SH, Montoro DT, Silverman CL, Shahin W, Zhao R, Jensen-Cody CW, Adamcakova-Dodd A, Evans TIA, Xie W, Zhang Y, Mou H, Herring BP, Thorne PS, Rajagopal J, Yeaman C, Parekh KR, Engelhardt JF (2018) Submucosal gland myoepithelial cells are reserve stem cells that can regenerate mouse tracheal epithelium. Cell Stem Cell 22(5):653-667.e5. doi: 10.1016/j.stem.2018.03.017 PMID: 29656941

Objective: To investigate whether a lineage relationship exists between two stem cell compartments that contribute to airway repair – basal cells in the surface airway epithelium (SAE) and an unknown submucosal gland (SMG) celltype.

Summary: Using lineage tracing of glandular myoepithelial cells (MECs), they demonstrate that MECs can give rise to seven cell types of the SAE and SMGs following severe airway injury. MECs progressively adopted a basal cell phenotype on the SAE and established asting progenitors capable of further regeneration following reinjury. MECs activate Wnt-regulated transcription factors (Lef-1/TCF7) following injury and Lef-1 induction in cultured MECs promoted transition to a basal cell phenotype. Surprisingly, dose-dependent MEC conditional activation of Lef-1in vivo promoted self-limited airway regeneration in the absence of injury.

Usage: immunofluorescence

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Sanvanson P, Li Z, Ward BD, Shaker R (2018) Mo1545 – Vagal nerve modulates the effects of esophageal acid on the periaqueductal gray functional connectivity in a rat model. Gastroenterology 154:S-747-S-748. doi: 10.1016/S0016-5085(18)32596-4

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Winston J (2018) Mo1546 – Neonatal colon inflammation-induced increases in pituitary adenylate cyclase activating peptide expression in the parabrachial nucleus contributes to reduced meal consumption, to increased meal-induced aversive and anxiety-like behaviors in adult rats. Gastroenterology 154:S-748. doi: 10.1016/S0016-5085(18)32597-6

Summary: Infusion of Anti-DBH-SAP into the dorsal vagal complex significantly increased meal volumes and increased open field activity compared to IgG-saporin treatment.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Pintus R, Riggi M, Cannarozzo C, Valeri A, de Leo G, Romano M, Gulino R, Leanza G (2018) Essential role of hippocampal noradrenaline in the regulation of spatial working memory and TDP‐43 tissue pathology. J Comp Neurol 526:1131-1147. doi: 10.1002/cne.24397

Objective: To determine the noradrenergic contribution to cognitive and histopathological changes in Alzheimer’s Disease.

Summary: Integrity of ascending noradrenergic inputs to the hippocampus may be required for the regulation of specific aspects of learning and memory and to prevent TDP-43 tissue pathology.

Usage: Anti-DBH-SAP was used at a dose of 0.50 µg dissolved in sterile PBS.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Meng J, Ni J, Wu Z, Jiang M, Zhu A, Qing H, Nakanishi H (2018) The critical role of IL-10 in the antineuroinflammatory and antioxidative effects of Rheum tanguticum on activated microglia. Oxid Med Cell Longev 2018:12. doi: 10.1155/2018/1083596

Objective: To investigate anti-inflammatory and antioxidative effects of a traditional Tibetan medicine (Rheum tanguticum; RT) on activated microglia.

Summary: RT may be useful for the pharmacological intervention against excessive inflammatory and oxidative responses associated with AD by inducing the production of IL-10 by microglia.

Usage: Mac-1-SAP (Cat. #IT-06, 1.3nM) was applied to hippocampal slice cultures 24 h prior to stimulation with chromogranin A or pancreastatin.

Related Products: Mac-1-SAP mouse/human (Cat. #IT-06)

Ogawa T, Li Y, Lua I, Hartner A, Asahina K (2018) Isolation of a unique hepatic stellate cell population expressing integrin α8 from embryonic mouse livers. 247(6):867-881. doi: 10.1002/dvdy.24634 PMID: 29665133

Objective: The aim of this study is to identify specific markers for embryonic Hepatic stellate cells (HSCs).

Summary: ITGA8 is a specific cell surface marker of MC-derived HSCs and perivascular mesenchymal cells in the developing liver. Our data suggest that ITGA8+ mesenchymal cells maintain the phenotype of hepatoblast in liver development.

Usage: Immunofluorescence (1:1000)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Ljubojevic V, Luu P, Gill PR, Beckett L-A, Takehara-Nishiuchi K, De Rosa E (2018) Cholinergic modulation of frontoparietal cortical network dynamics supporting supramodal attention. J Neurosci 38:3988-4005. doi: 10.1523/JNEUROSCI.2350-17.2018

Objective: To examine whether neurochemical acetylcholine (ACh) contributes to a state of readiness for target detection, by engaging frontoparietal cortical attentional networks independent of modality.

Summary: ACh supported alerting attention to an impending presentation of either visual or olfactory targets. Enhanced stimulus detection was associated with an anticipatory increase in power in the beta oscillation range prior to the target’s appearance within the posterior parietal cortex (PPC) as well as increased synchrony. In beta, this was also detected between the prefrontal cortex and PPC. ACh may act, in a supramodal manner, to prepare frontoparietal cortical attentional networks for target detection.

Usage: The ACh-NBM-lesioned rats received an injection of 0.2 μl of 0.3 μg/μl 192 IgG-SAP

Related Products: 192-IgG-SAP (Cat. #IT-01)

Zhang Q, De Corte B, Jung D, Kim Y, Geerling J, Narayanan N (2018) Cholinergic modulation targeting medial prefrontal cortex leads to behavior deficit in interval timing task. Neurology 90 (15 Supplement):P5.195.

Objective: To determine the effect of cholinergic lesion targeting medial prefrontal cortex on interval timing behavior.

Summary: Mice receiving medial prefrontal mu-p75-saporin injection performed poorly compared to control mice in interval timing task. Cholinergic lesion targeting medial prefrontal cortex caused interval timing behavior deficit in wild type mice.

Usage: mu-p75-SAP, a toxin targeting cholinergic neurons, into the bilateral medial prefrontal cortical regions of wild type mice pre-trained in interval timing task. Control mice (also pre-trained in interval timing task, n=8) received stereotactic injection of Rabbit IgG-SAP.

Related Products: mu p75-SAP (Cat. #IT-16), Rabbit IgG-SAP (Cat. #IT-35)