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Labeling neuronal proteins with quantum dots for single-molecule imaging
Thal LB, Kovtun O, Rosenthal SJ (2020) Labeling neuronal proteins with quantum dots for single-molecule imaging. (eds. Fontes A, Santos B). In: Quantum Dots. Methods in Molecular Biology. 2135:169-177. Humana Press, New York, NY. doi: 10.1007/978-1-0716-0463-2_9 PMID: 32246334
Objective: The authors describe how nanometer-sized fluorescent semiconductors called quantum dots (QD) can be used to label neuronal proteins in a single QD imaging format.
Usage: Secondary antibody-conjugated QDs target membrane proteins pre-treated with SERT Mouse Monoclonal.
Related Products: SERT Mouse Monoclonal (Cat. #AB-N40)
Intracerebroventricular administration of 192IgG-saporin alters the state of microglia in the neocortex.
Volobueva MN, Dobryakova YV, Manolova AO, Stepanichev MY, Kvichansky AA, Gulyaeva NV, Markevich VA, Bolshakov AP (2020) Intracerebroventricular administration of 192IgG-saporin alters the state of microglia in the neocortex. Neurochem J 14(1):37-42. doi: 10.1134/S1819712420010213
Objective: The effect of intracerebroventricular (icv) immunotoxin administration on the state of microglia in tissues adjacent to the ventricle (striatum and parietal cortex) and remotely located but receiving innervation from the medial septal region and diagonal band of Broca (entorhinal cortex and olfactory bulbs).
Summary: 1.5 months after the administration of immunotoxin, microglia are activated only in the neocortical areas, not in the striatum or olfactory bulbs.
Usage: Injected bilaterally at a dose of 4 μg in each ventricle.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Thyroid hormone in the pathogenesis of congenital intestinal dysganglionosis
Wang F, Jing P, Zhan P, Zhang H (2020) Thyroid hormone in the pathogenesis of congenital intestinal dysganglionosis. Pediatr Dev Pathol 23(4):285-295. doi: 10.1177/1093526620908984 PMID: 32212960
Objective: To investigate the role of thyroid hormone (TH) in the pathogenesis of intestinal dysganglionosis (ID).
Summary: 6-propyl-2-thiouracil (PTU) inhibited TH production, reduced the number of enteric neurons, impaired intestinal motility, and impeded ENCC mitosis in zebrafish, suggesting a possible role of CH in the pathogenesis of ID.
Usage: Immunostaining (1:60); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Exploiting the internalization feature of an antibody against the glycosphingolipid SSEA-4 to deliver immunotoxins in breast cancer cells
Ruggiero FM, Rodríguez-Walker M, Daniotti JL (2020) Exploiting the internalization feature of an antibody against the glycosphingolipid SSEA-4 to deliver immunotoxins in breast cancer cells. Immunol Cell Biol 98(3):187-202. doi: 10.1111/imcb.12314 PMID: 31916611
Cholinergic basal forebrain degeneration due to obstructive sleep apnoea increases Alzheimer’s pathology in mice.
Qian L, Kasas L, Milne MR, Rawashdeh O, Marks N, Sharma A, Bellingham MC, Coulson EJ (2020) Cholinergic basal forebrain degeneration due to obstructive sleep apnoea increases Alzheimer’s pathology in mice. bioRxiv 2020.03.12.989848. doi: 10.1101/2020.03.12.989848
Usage: bilateral injections of urotensin II-saporin (UII-SAP; 0.07 μg/μL per site – unless stated otherwise; generous gift from Advanced Targeting Systems)
Related Products: Custom Conjugates, Blank-SAP (Cat. #IT-21)
Early forebrain neurons and scaffold fibers in human embryos.
Qin J, Wang M, Zhao T, Xiao X, Li X, Yang J, Yi L, Goffinet AM, Qu Y, Zhou L (2020) Early forebrain neurons and scaffold fibers in human embryos. Cerebral Cortex 30(3):913–928. doi: 10.1093/cercor/bhz136 PMID: 31298263
Objective: To study neural progenitor proliferation, neuronal migration, areal organization, and pioneer axon wiring are critical events during early forebrain development.
Summary: Axons from pioneer neurons in prethalamus, ventral telencephalon, and cortical preplate cross the diencephalon–telencephalon junction and the pallial–subpallial boundary, forming scaffolds that could guide thalamic and cortical axons at later stages.
Usage: Immunofluorescence (1:600); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal, affinity-purified
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors.
Barry DM, Liu XT, Liu B, Liu XY, Gao F, Zeng X, Liu J, Yang Q, Wilhelm S, Yin J, Tao A, Chen ZF (2020) Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors. Nat Commun 11(1):1397. doi: 10.1038/s41467-020-15230-y
Objective: To determine the role of GRP in sensory neurons.
Summary: GRP is a neuropeptide in sensory neurons for nonhistaminergic itch, and GRP sensory neurons are dedicated to itch transmission.
Usage: Bombesin-SAP (200 ng/5 μL, i.t.) was injected 2 weeks prior to optical stimulation.
Related Products: Bombesin-SAP (Cat. #IT-40)
Saponin facilitates anti-robo1 immunotoxin cytotoxic effects on maxillary sinus squamous cell carcinoma
Komatsu N, Komatsu M, Ohashi R, Horii A, Hoshi K, Takato T, Abe T, Hamakubo T (2020) Saponin facilitates anti-robo1 immunotoxin cytotoxic effects on maxillary sinus squamous cell carcinoma. J Oncol 2020:9593516. doi: 10.1155/2020/9593516 PMID: 32256588
Objective: To examine whether a flashed treatment with saponin exerted a significant specific cytotoxic effect on cancer cells.
Summary: The authors have shown previously the enhanced cytotoxic effects of saporin-conjugated anti-Robo1 immunotoxin (IT-Robo1) on the HNSCC cell line HSQ-89 in combination with a photochemical internalization technique. The combination of an immunotoxin with saponin also exhibited a significant tumor-suppressive effect on mice HSQ-19 xenograft.
Usage: A saporin-conjugated anti-Robo1 antibody (IT-Robo1) and saporin-conjugated negative control antibody (IT-NC), were prepared by incubating 121μl of 1.1μM streptavidin-saporin and 138μl of 1.1μM biotinylated monoclonal antibodies for 30 min at room temperature. HSQ-89 cells were seeded at 2.0×104cells per well in 96-well plates and cultured overnight. On the following day, they were exposed to various concentrations (0.054 pM∼4.2 nM) of either IT-Robo1 or IT-NC.
Related Products: Streptavidin-ZAP (Cat. #IT-27)
Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors.
Bernabe CS, Caliman IF, Truitt WA, Molosh AI, Lowry CA, Hay-Schmidt A, Shekhar A, Johnson PL (2020) Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors. J Psychopharmacol 34(4):400-411. doi: 10.1177/0269881119900981
Objective: To investigate the role of amygdala-projecting 5-HT neurons in the DR in innate anxiety and conditioned fear behaviors.
Summary: The studies support the hypothesis that amygdala-projecting 5-HT neurons in the DR represent an anxiety and fear-on network.
Usage: Each rat received two bilateral microinjections per site (100 nL each, 1 μM in artificial cerebrospinal fluid) of either Anti-SERT-SAP or the control Mouse IgG-SAP.
Related Products: Anti-SERT-SAP (Cat. #IT-23), Mouse IgG-SAP (Cat. #IT-18)
YY1-mediated overexpression of long noncoding RNA MCM3AP-AS1 accelerates angiogenesis and progression in lung cancer by targeting miR-340-5p/KPNA4 axis.
Li X, Yu M, Yang C (2020) YY1-mediated overexpression of long noncoding RNA MCM3AP-AS1 accelerates angiogenesis and progression in lung cancer by targeting miR-340-5p/KPNA4 axis. J Cell Biochem 121(3):2258-2267. doi: 10.1002/jcb.29448 PMID: 31693222
Usage: western (1:1000)
Related Products: Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)