References

Jia M (2020) Vascular-cognitive impairment after chronic high-thoracic spinal cord injury. University of British Columbia, Experimental Medicine Thesis. PMID: 0

Objective: To investigate whether chronic disruption of cardiovascular control following experimental spinal cord injury (SCI) results in cerebrovascular decline and vascular cognitive impairment.

Summary: Chronic experimental SCI is associated with structural alteration and endothelial dysfunction in cerebral arteries that likely contributes to significantly reduced CBF and vascular cognitive impairment.

Usage: Immunofluorescence (1:600)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Hoffmann RM, Mele S, Cheung A, Larcombe-Young D, Bucaite G, Sachouli E, Zlatareva I, Morad HOJ, Marlow R, McDonnell JM, Figini M, Lacy KE, Tutt AJN, Spicer JF, Thurston DE, Karagiannis SN, Crescioli S (2020) Rapid conjugation of antibodies to toxins to select candidates for the development of anticancer antibody-drug conjugates (ADCs). Sci Rep 10(1):8869. doi: 10.1038/s41598-020-65860-x

Objective: To facilitate rapid and cost-effective screening of antibody and toxin combinations for activity and safety, based on streptavidin-biotin conjugation

Summary: For antibody selection, the authors evaluated internalization by target cells using streptavidin-linked antibodies conjugated to biotinylated saporin, a toxin unable to cross cell membranes. For payload selection, the authors biotinylated toxins and conjugated them to antibodies linked to streptavidin to evaluate antitumour activity and pre-clinical safety.

Usage: Streptavidin-linked antibody was incubated for 30min with biotinylated Saporin (BT-ZAP) at a molar ratio of 1:6.

Related Products: Streptavidin-ZAP (Cat. #IT-27), MonoBiotin-ZAP (Cat. #BT-ZAP)

Xiao D, Deng Q, Guo Y, Huang X, Zou M, Zhong J, Rao P, Xu Z, Liu Y, Hu Y, Shen Y, Jin K, Xiang M (2020) Generation of self-organized sensory ganglion organoids and retinal ganglion cells from fibroblasts. Sci Adv 6(22):eaaz5858. doi: 10.1126/sciadv.aaz5858 PMID: 32523990

Objective: To examine whether sensory ganglion (SG) organoids can be reprogrammed by transcription factors (TFs) into sensory ganglion (SG) neurons.

Summary: This study identifies a combination of triple TFs Ascl1, Brn3b/3a, and Isl1 (ABI) as an efficient means to reprogram mouse and human fibroblasts into self-organized and networked induced SG (iSG) organoids. iSG organoids may serve as a model to decipher the pathogenesis of sensorineural diseases and screen effective drugs and a source for cell replacement therapy.

Usage: Immunohistochemistry, Immunocytochemistry (1:500); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Easton A, Barros M, Lever C (2020) Acetylcholine and spontaneous recognition memory in rodents and primates. Curr Top Behav Neurosci 45:29-45. doi: 10.1007/7854_2020_132

Summary: Review of lesioning designed to specifically target cells that express acetylcholine as a transmitter.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Parikh V, Bangasser DA (2020) Cholinergic signaling dynamics and cognitive control of attention. Shoaib M., Wallace T (Ed.): Behavioral Pharmacology of the Cholinergic System. Current Topics in Behavioral Neurosciences. 45:71-87. Springer, Cham doi: 10.1007/7854_2020_133

Summary: A plethora of studies conducted in rodents demonstrated that selective lesions of BF cholinergic neurons and their cortical inputs produced by the immunotoxin 192-IgG-SAP impair performance in various tasks of attention.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Qi R, Li TC, Chen X (2020) The role of the renin-angiotensin system in regulating endometrial neovascularization during the peri-implantation period: literature review and preliminary data. Ther Adv Endocrinol Metab 11:2042018820920560. doi: 10.1177/2042018820920560 PMID: 32499907

Objective: The role of the renin–angiotensin system (RAS) in the peri-implantation endometrium.

Summary: Altered vasculature of the endometrium in the peri-implantation period is detrimental to implantation and may lead to recurrent miscarriage. Being an angiogenic mediator, endometrial RAS may play a role around the time of embryo implantation, affecting subsequent pregnancy outcomes.

Usage: Immunohistochemistry (1:100)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Souza-Silva E, Stein T, Mascarin LZ, Dornelles FN, Bicca MA, Tonussi CR (2020) Intra-articular injection of 2-pyridylethylamine produces spinal NPY-mediated antinociception in the formalin-induced rat knee-joint pain model. Brain Res 1735:146757. doi: 10.1016/j.brainres.2020.146757 PMID: 32135147

Objective: To investigate the participation of spinal NPY in the antinociceptive effect produced by 2-pyridylethylamine (2-PEA).

Summary: These data support the idea that antinociception induced by H1R agonists in the knee-joint of rats may be mediated by the spinal release of NPY, and this peptide seems to be acting via Y1R.

Usage: IT pretreatment with NPY-SAP (750 ng) reduced immunoblotting for Y1R in spinal cord homogenates.

Related Products: NPY-SAP (Cat. #IT-28)

Vasquez JH, Borniger JC (2020) Neuroendocrine and behavioral consequences of hyperglycemia in cancer. Endocrinology 161(5):bqaa047. doi: 10.1210/endocr/bqaa047 PMID: 32193527

Summary: Ablation of norepinephrine containing projections to the arcuate (via Anti-DBH-SAP injections) alters AgRP and neuropeptide (NPY) concentrations, leading to impairments in hypoglycemic (glucoprivic) or ghrelin-induced feeding.

Usage: Anti-DBH-SAP (bilateral 42-ng intracranial injections) was used in rats to investigate the role of hindbrain catecholamine afferents in increased ARC NPY and AgRP gene expression.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

See Also:

• Fraley GS et al. Immunolesion of norepinephrine and epinephrine afferents to medial hypothalamus alters basal and 2-deoxy-D-glucose-induced neuropeptide Y and agouti gene-related protein messenger ribonucleic acid expression in the arcuate nucleus. Endocrinology 144(1):75-83, 2003.

Harris RBS (2020) Loss of leptin receptor-expressing cells in the hindbrain decreases forebrain leptin sensitivity. Am J Physiol Endocrinol Metab 318(5):E806-E816. doi: 10.1152/ajpendo.00020.2020

Objective: This study tested whether loss of hindbrain leptin receptor signaling changed sensitivity to forebrain leptin.

Summary: Loss of VMH (ventromedial nucleus of hippocampus) leptin receptor-expressing cells prevents weight loss. The integrated response between the hindbrain and forebrain is heavily dependent upon leptin activity in the VMH.

Usage: To test forebrain leptin sensitivity Leptin-SAP and Blank-SAP rats received third ventricle injections of 0, 0.05, 0.1, 0.25 or 0.5 μg leptin.

Related Products: Leptin-SAP (Cat. #IT-47), Blank-SAP (Cat. #IT-21)

Silva JDN, Oliveira LM, Souza FC, Moreira TS, Takakura AC (2020) GABAergic neurons of the medullary raphe regulate active expiration during hypercapnia. J Neurophysiol 123(5):1933-1943. doi: 0.1152/jn.00698.2019 PMID: 32267190

Objective: To test the hypothesis that the medullary raphe, a region known to be involved in breathing, also contributes to expiratory activity under hypercapnic conditions.

Summary: Depletion of serotonergic neurons with Anti-SERT-SAP (IT-23) injections into raphe obscurus (ROb) and magnus (RMg) did not increase the amplitude of abdominal activity during hypercapnia. These results show that the presumably GABAergic neurons within the RMg and ROb may be the inhibitory source to modulate the activity of the parafacial respiratory group during hypercapnia condition.

Usage: Intraparenchymal injections of Anti-SERT-SAP (IT-23) were injected into ROb or RMg at (10 µg/µl).

Related Products: Anti-SERT-SAP (Cat. #IT-23)