References

Seven YB, Mitchell GS (2019) Mechanisms of compensatory plasticity for respiratory motor neuron death. Respir Physiol Neurobiol 265:32-39. doi: 10.1016/j.resp.2019.01.001

Summary: Discusses recent advances in understanding of mechanisms giving rise to compensatory respiratory plasticity in response to respiratory motor neuron death.

Related Products: CTB-SAP (Cat. #IT-14)

Koshy Cherian A, Kucinski A, Wu R, deJong IEM, Sarter M (2019) Co-treatment with rivastigmine and idalopirdine reduces the propensity for falls in a rat model of falls in Parkinson’s disease. Psychopharmacology 236:1701–1715. doi: 10.1007/s00213-018-5150-y

Objective: The authors used a longer and more taxing rotating beam apparatus to determine the potential therapeutic efficacy of idalopirdine when combined with rivastigmine.

Summary: The results extend the prediction that the combined treatment with idalopirdine and an AChE inhibitor improves complex movement control and reduces propensity for falls in patients with movement disorders.

Usage: Basal forebrain cholinergic neurons situated in the nucleus basalis and substantia innominata were targeted with 192-IgG-SAP in aCSF infused bilaterally (200 ng/μL; 0.8 μL/hemisphere).

Related Products: 192-IgG-SAP (Cat. #IT-01)

Saika F, Kiguchi N, Matsuzaki S, Kobayashi D, Kishioka S (2019) Inflammatory macrophages in the sciatic nerves facilitate neuropathic pain associated with type 2 diabetes mellitus. J Pharmacol Exp Ther 368(3):535-544. doi: 10.1124/jpet.118.252668

Objective: To determine whether inflammatory macrophages contribute to neuropathic pain associated with type 2 diabetes-mellitus (T2DM).

Summary: Inhibitory agents for macrophage-driven neuroinflammation could be potential candidates for novel pharmacotherapy against intractable neuropathic pain.

Usage: Injections of Mac-1-SAP or unconjugated Saporin (10 μl) were administered 3 times every 2 days. Perineural administration of Mac-1-SAP improved high-fat diet (HFD)-induced mechanical allodynia and the accumulation of F4/80+ macrophages and the upregulation of inflammatory mediators in the SCN after HFD-feeding.

Related Products: Mac-1-SAP mouse/human (Cat. #IT-06), Saporin (Cat. #PR-01)

Wang Y, Li G, Wang X, Zhu S (2019) Effects of Shugan Hewei Granule on depressive behavior and protein expression related to visceral sensitivity in a rat model of nonerosive reflux disease. Evid Based Complement Alternat Med 2019:1505693. doi: 10.1155/2019/1505693 PMID: 30719054

Usage: immunofluorescent staining (1:200)

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)

Gao ZR, Chen WZ, Liu MZ, Chen XJ, Wan L, Zhang XY, Yuan L, Lin JK, Wang M, Zhou L, Xu XH, Sun YG (2019) Tac1-expressing neurons in the periaqueductal gray facilitate the itch-scratching cycle via descending regulation. Neuron 101(1):45-59.e9. doi: 10.1016/j.neuron.2018.11.010

Objective: To determine the neural mechanism promoting the itch-scratching cycle.

Summary: Ablation of Tac1+ but not SST+ neurons decreases itch-induced scratching behavior. l/vlPAG Tac1+ neurons Induce Scratching Behavior via a Descending Pathway.

Usage: To ablate the spinal GRPR+ neurons, mice were intrathecally injected with Bombesin-SAP or Control Blank-SAP (400 ng/5 mL).

Related Products: Bombesin-SAP (Cat. #IT-40), Blank-SAP (Cat. #IT-21)

Sweeney NT, James KN, Nistorica A, Lorig-Roach RM, Feldheim DA (2019) Expression of transcription factors divides retinal ganglion cells into distinct classes. J Comp Neurol 527(1):225-235. doi: 10.1002/cne.24172 PMID: 28078709

Usage: Melanopsin (Opn4) Immunostaining 1:1000

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Quattrochi LE, Stabio ME, Kim I, Ilardi MC, Michelle Fogerson P, Leyrer ML, Berson DM (2019) The M6 cell: A small-field bistratified photosensitive retinal ganglion cell. J Comp Neurol 527(1):297-311. doi: 10.1002/cne.24556 PMID: 30311650

Summary: M6 cells express low levels of melanopsin and have correspondingly weak intrinsic light responses.

Usage: In all experiments involving melanopsin immunofluorescence, melanopsin immunodetection was enhanced using tyramide signal amplification coupled with horseradish peroxidase (HRP)-paired with goat anti-rabbit IgG and an Alexa fluorophore. 1:10,000.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Dhande OS, Seabrook TA, Phan AH, Salaly LD, Ishiko N, Nguyen PL, Wang JT, Evans SM, Huberman AD (2018) Assembly of functionally antagonistic visual circuits for controlling pupil dynamics. bioRxiv 500868. doi: 10.1101/500868

Objective: Investigate the mechanisms controlling the specification and establishment of parallel sensory pathways.

Summary: Identification of a novel genetic program that marks and is required for the development of non-image-forming parallel visual pathways.

Usage: Immunohistochemistry (1:1000)

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Säwen P, Eldeeb M, Erlandsson E, Kristiansen TA, Laterza C, Kokaia Z, Karlsson G, Yuan J, Soneji S, Mandal PK, Rossi DJ, Bryder D (2018) Murine HSCs contribute actively to native hematopoiesis but with reduced differentiation capacity upon aging. Elife 7:e41258. doi: 10.7554/elife.41258 PMID: 30561324

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Berhani O, Glasner A, Kahlon S, Duev-Cohen A, Yamin R, Horwitz E, Enk J, Moshel O, Varvak A, Porgador A, Jonjic S, Mandelboim O. (2019) Human anti-NKp46 antibody for studies of NKp46-dependent NK cell function and its applications for type 1 diabetes and cancer research. Eur J Immunol 49(2):228-241. doi: 10.1002/eji.201847611 PMID: 30536875

Objective: To investigate human NKp46 activity and its critical role in Natural Killer (NK) cell biology.

Summary: A unique anti-human NKp46 monoclocal antibody was developed and conjugated to Saporin. Targeted toxin inhibits growth of NKp46-positive cells; thus, exemplifying the potential as an immunotherapeutic drug to treat NKp46-dependent diseases, such as, type I diabetes and NK and T cell related malignancies.

Usage: Conjugation of the antibodies to Saporin, treatment of cells, and cell viability assay Biotin-Z Kit instructions.

Related Products: Streptavidin-ZAP (Cat. #IT-27)