References

Benade V, Daripelli S, Tirumalasetty C, Subramanian R, Petlu S, Badange R, Nirogi R (2019) 0054 SUVN-G3031, a histamine H3 receptor inverse agonist produces wake promoting effect in orexin-2-saporin lesioned rats. Sleep 42(Supplement_1):A22-A23. doi: 10.1093/sleep/zsz067.053

Summary: Rats lesioned with Orexin-SAP in lateral hypothalamus produced narcoleptic-like behavior.

Related Products: Orexin-B-SAP (Cat. #IT-20)

Deura C, Minabe S, Ikegami K, Inoue N, Uenoyama Y, Maeda KI, Tsukamura H (2019) Morphological analysis for neuronal pathway from the hindbrain ependymocytes to the hypothalamic kisspeptin neurons. J Reprod Dev 65(2):129-137. doi: 10.1262/jrd.2018-122

Objective: To examine the existence of a neuronal pathway from the hindbrain ependymocytes to kisspeptin neurons in the arcuate nucleus (ARC) and anteroventral periventricular nucleus (AVPV).

Summary: The hindbrain ependymocytes have neuronal connections with the kisspeptin neurons, most probably via hindbrain noradrenergic and CRH neurons to relay low energetic signals for regulation of reproduction.

See: I’Anson H et al. Immunotoxic destruction of distinct catecholaminergic neuron populations disrupts the reproductive response to glucoprivation in female rats. Endocrinology 144(10):4325-4331, 2003.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Urquhart MA, Ross JA, Reyes BAS, Nitikman M, Thomas SA, Mackie K, Van Bockstaele EJ (2019) Noradrenergic depletion causes sex specific alterations in the endocannabinoid system in the Murine prefrontal cortex. Neurobiology of Stress 10:100164. doi: 10.1016/j.ynstr.2019.100164

Objective: To determine the effects of NE depletion on the eCB system.

Summary: The results suggest that the endocannabinoids (eCB) system may be more responsive in males than females under conditions of NE perturbation, thus having potential implications for sex-specific treatment strategies of stress-related psychiatric disorders.

Usage: The authors used a DBH knockout model and DSP-4 (a compound that depletes endogenous norepinephrine and enhances release of [3H]norepinephrine). More specific mechanisms for further corroboration could be undertaken, such as using Anti-DBH-SAP to provide a more complete lesion compared to DSP-4.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Abudukeyoumu N, Garcia-Munoz M, Nakano Y, Arbuthnott GW (2018) Featured Article: Impaired reach-to-grasp responses in mice depleted of striatal cholinergic interneurons. Targeting Trends 19

Related Products: Anti-ChAT-SAP (Cat. #IT-42)

Read the featured article in Targeting Trends.

See Also:

• Abudukeyoumu N et al. Impaired reach-to-grasp responses in mice depleted of striatal cholinergic interneurons. Neuroscience 2018 Abstracts 491.01 / MM13, 2018. Society for Neuroscience, San Diego, CA

Manavella DD, Cacciottola L, Payen VL, Amorim CA, Donnez J, Dolmans MM (2019) Adipose tissue-derived stem cells boost vascularization in grafted ovarian tissue by growth factor secretion and differentiation into endothelial cell lineages. Mol Hum Reprod 25(4):184-193. doi: 10.1093/molehr/gaz008 PMID: 30824937

Usage: immunohistochemistry (1:2000)

Related Products: Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)

Toledo C, Andrade DC, Del Rio R (2019) Brainstem pre-sympathetic neurons contribute to irregular breathing patterns in volume overload heart failure. FASEB J 33(1):lb630. Experimental Biology 2019 Meeting Abstracts doi: 10.1096/fasebj.2019.33.1_supplement.lb630

Objective: To investigate the contribution of RVLM-C1 neurons on breathing disorders in chronic heart failure (CHF).

Summary: RVLM-C1 neurons play a critical role in the maintenance of altered breathing patterns in CHF rats and highlighted their contribution to the worsening of cardiac function during central chemoreflex activation. DBH-SAP treatment decreased active expiration in CHF rats and deleterious effects of central chemoreflex activation on diastolic cardiac function and cardiac autonomic control were blunted.

Usage: Stereotaxic bilateral injections of Anti-DBH-SAP (5 ng/150 nl).

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Sajjadi E, Seven YB, Simon AK, Zwick A, Satriotomo I, Mitchell GS (2019) Adenosine 2A receptor inhibition promotes neuroprotection following toxic insult to phrenic motor neurons. FASEB J 33(1):844.3. Experimental Biology 2019 Meeting Abstracts doi: 10.1096/fasebj.2019.33.1_supplement.844.3

Objective: The authors explored the role of A2A receptors in phrenic motor neuron cell death in vivo.

Summary: A2A receptors, which contribute to motor neuron death during toxic insults, are upregulated in spared phrenic motor neurons of CTB-SAP treated rats. This is an important finding since A2A receptor upregulation may accelerate motor neuron death in neurodegenerative diseases like ALS.

Usage: CTB-SAP selectively killed nearly all phrenic motor neurons within a week and caused diaphragm paralysis (p<0.01).

Related Products: CTB-SAP (Cat. #IT-14)

Borkowski LF, Nichols NL (2019) A2A and 5‐HT receptors are differentially required for respiratory plasticity over the course of motor neuron loss in intrapleurally CTB-SAP treated rats. FASEB J 33(1):843.3. Experimental Biology 2019 Meeting Abstracts doi: 10.1096/fasebj.2019.33.1_supplement.843.3

Objective: To investigate the role of serotonin (5-HT) and adenosine 2A (A2A) receptors in respiratory plasticity.

Summary: A2A receptors are necessary for respiratory plasticity early (7d), but 5-HT receptors are required late (28d).

Usage: Bilateral, intrapleural injections of: 1) CTB-SAP (25 μg), or 2) un-conjugated CTB and SAP (control) in rats.

Related Products: CTB-SAP (Cat. #IT-14)

Krajewski-Hall SJ, Miranda Dos Santos F, McMullen NT, Blackmore EM, Rance NE (2019) Glutamatergic neurokinin 3 receptor neurons in the median preoptic nucleus modulate heat-defense pathways in female mice. Endocrinology 160(4):803-816. doi: 10.1210/en.2018-00934

Objective: To characterize the thermoregulatory role of MnPO NK3R neurons in female mice.

Summary: Study suggests that KNDy neurons modulate thermosensory pathways for heat defense indirectly via a subpopulation of glutamatergic MnPO neurons that express NK3R.

Usage: Mice were bilaterally injected with 10 ng NK3-SAP in 100 nL PBS (n = 14) or blank-SAP (n = 8) in the preoptic area adjacent to the MnPO.

Related Products: NKB-SAP (Cat. #IT-63), Blank-SAP (Cat. #IT-21)

Amano M, Amiya N, Yamamoto N, Osugi T, Tsutsui K (2019) Immunohistochemical detection of prolactin-releasing peptide2 in the brain of the inshore hagfish Eptatretus burgeri. Gen Comp Endocrinol 274:1-7. doi: 10.1016/j.ygcen.2018.12.005 PMID: 30571962

Objective: To identify Prolactin-releasing peptide2 (PrRP2) in hagfish.

Summary: The study demonstrates, for the first time, that PrRP2 is expressed in the brain of inshore hagfish. The restricted distribution of PrRP2-ir fibers in the HYinf suggests that PrRP2 does not directly regulate the pituitary gland, but regulates the function of the HYinf where PQRFa and CRH are expressed.

Usage: Immunohistochemistry (1:1000). The authors clarified the cross-reactivity of the antibody against other CRH family peptides, such as urocortin-I, II, III, urotensin-I, and sauvagine: the cross-reactivity of anti-CRH against CRH family peptides was found to be less than 0.01%, indicating the specificity of the antibody.

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)