References

Kitahama K, Araneda S, Geffard M, Sei H, Okamura H (2005) Tyramine-immunoreactive neuronal structures in the rat brain: abundance in the median eminence of the mediobasal hypothalamus. Neurosci Lett 383(3):215-219. doi: 10.1016/j.neulet.2005.04.029 PMID: 15955414

Related Products: Tyramine Rabbit Polyclonal, Conjugated (Cat. #AB-T071)

Wiley RG, Lappi DA (2005) Molecular neurosurgery with targeted toxins. Humana Press, Totowa, New Jersey.

Summary: The idea behind the book was to provide a road map for the users of Molecular Neurosurgery to see how experienced scientists used these exceptional reagents in their work. Experiments with several targeted toxins are described, and readers can get an idea either specifically about a targeted toxin that they’re using, or about how a type of molecule is used and at what dosage, in a paradigm similar to theirs.

Related Products: 192-IgG-SAP (Cat. #IT-01), ME20.4-SAP (Cat. #IT-15), Anti-DBH-SAP (Cat. #IT-03), SSP-SAP (Cat. #IT-11), Dermorphin-SAP / MOR-SAP (Cat. #IT-12), IB4-SAP (Cat. #IT-10), CTB-SAP (Cat. #IT-14)

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Kim I, Wilson RE, Wellman CL (2005) Aging and cholinergic deafferentation alter GluR1 expression in rat frontal cortex. Neurobiol Aging 26(7):1073-1081. doi: 10.1016/j.neurobiolaging.2004.09.005

Summary: Neuronal plasticity is involved in several processes during adulthood, including learning and memory, and recovery from injury. Recent evidence suggests that aging reduces this plasticity. The authors used 0.15 µg injections of 192-Saporin (Cat. #IT-01) into the nucleus basalis magnocellularis of rats to investigate how the loss of cortical plasticity would affect the expression of GluR1. Younger animals displayed a marked increase in the number of GluR1-expressing neurons, a compensatory response not seen in older animals.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Harrell LE, Parsons DS, Kolasa K (2005) Pro- and anti-apoptotic evidence for cholinergic denervation and hippocampal sympathetic ingrowth in rat dorsal hippocampus. Exp Neurol 194(1):182-190. doi: 10.1016/j.expneurol.2005.02.009

Summary: Cholinergic denervation of the hippocampus results in hippocampal sympathetic ingrowth (HSI) of fibers from the superior cervical ganglion; this ingrowth may exert an anti-apoptotic effect. After 1-µg injections of 192-Saporin (Cat. #IT-01) into the medial septum of rats, the authors investigated the levels of apoptotic protein expression and DNA fragmentation. The findings suggest that cholinergic denervation causes pro-apoptotic responses, but HSI exerts a protective effect against programmed cell death.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Pappas BA, Payne KB, Fortin T, Sherren N (2005) Neonatal lesion of forebrain cholinergic neurons: Further characterization of behavioral effects and permanency. Neuroscience 133(2):485-492. doi: 10.1016/j.neuroscience.2005.02.040

Summary: Neonatal rats treated with bilateral intracerebroventricular injections of 300 ng of 192-Saporin (Cat. #IT-01) showed basal forebrain cholinergic neuron loss that was still evident at 24 months of age. The authors tested the reference memory and attentional processing of these rats in a Morris water maze. The results suggest that impaired performance of the treated animals in complex maze tasks reflects reduced problem solving ability rather than a deficit in attentional processing.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Abe T, Ohshita N, Sugiyo S, Moritani M, Kobayashi M, Takemura M (2005) Elimination of neurokinin-1 receptor neurons in caudal nucleus reverses the effects of systemic bicuculline on c-Fos expression in rat trigeminal sensory nucleus: I. High intensity electrical stimulation of the trigeminal ganglion. Neuroscience 133(3):739-747. doi: 10.1016/j.neuroscience.2005.03.021

Summary: The authors investigated the role of NK-1r + neurons in lamina 1 of the trigeminal caudal nucleus (Vc) for ora-facial nociception. After a 5 µl injection of 5 µM SP-SAP (Cat. #IT-07) into the cisterna magna, c-fos expression in the Vc was evaluated. SP-SAP treatment, along with use of bicuculline, a GABAA receptor antagonist, showed that NK-1r+ neurons in laminae I and III of the Vc are involved in nociceptive processing in the trigeminal sensory nucleus.

Related Products: SP-SAP (Cat. #IT-07)

Pagliardini S, Ren J, Wevrick R, Greer JJ (2005) Developmental abnormalities of neuronal structure and function in prenatal mice lacking the prader-willi syndrome gene necdin. Am J Pathol 167(1):175-191. doi: 10.1016/S0002-9440(10)62964-1 PMID: 15972963

Related Products: Antibody to NK-1 Receptor (Cat. #AB-N04)

Zhang RX, Wang L, Liu B, Qiao JT, Ren K, Berman BM, Lao L (2005) Mu opioid receptor-containing neurons mediate electroacupuncture-produced anti-hyperalgesia in rats with hind paw inflammation. Brain Res 1048(1-2):235-240. doi: 10.1016/j.brainres.2005.05.008

Summary: Electroacupuncture has been shown to significantly reduce inflammatory hyperalgesia. To examine whether this effect is modulated by spinal mu opioid receptors, the authors injected 400 ng of dermorphin-SAP (Cat. #IT-12) into the subarachnoid space at the level of the lumbar spinal cord of rats. The anti-hyperalgesic effect of electroacupuncture was blocked by dermorphin-SAP administration, indicating that mu opioid receptor-containing neurons are involved in this pathway.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12)

Smith HR, Pang KC (2005) Orexin-saporin lesions of the medial septum impair spatial memory. Neuroscience 132(2):261-271. doi: 10.1016/j.neuroscience.2004.12.037

Summary: The medial septum and diagonal band of Broca (MSDB) have been shown to be important for spatial learning and memory. The authors investigated the role orexin-containing neurons from the hypothalamus play in these processes. Rats were treated with three injections of 40-120 ng of orexin-SAP (Cat. #IT-20) into the MSDB. Performance in spatial working and spatial reference memory tasks indicate that orexin innvervation of the MSDB may modulate spatial memory through both GABAergic and cholinergic septohippocampal neurons.

Related Products: Orexin-B-SAP (Cat. #IT-20)

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Paban V, Jaffard M, Chambon C, Malafosse M, Alescio-Lautier B (2005) Time course of behavioral changes following basal forebrain cholinergic damage in rats: Environmental enrichment as a therapeutic intervention. Neuroscience 132(1):13-32. doi: 10.1016/j.neuroscience.2004.11.024

Summary: In this study the authors examined the effects of 192-Saporin (Cat. #IT-01) administration to the medial septum (37.5 ng/side) and nucleus basalis magnocellularis (75 ng/side) of rats. The results suggest that behavioral deficits immediately after lesioning are due to cholinergic depletion, while deficits later in life may be connected to a gradual degeneration process. Environmental enrichment had a significant positive effect on lesioned rats, indicating a level of cognitive plasticity.

Related Products: 192-IgG-SAP (Cat. #IT-01)