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Immunohistochemical detection of corticotropin-releasing hormone (CRH) in the brain and pituitary of the hagfish, Eptatretus burgeri.
Amano M, Amiya N, Yokoyama T, Onikubo K, Yamamoto N, Takahashi A (2016) Immunohistochemical detection of corticotropin-releasing hormone (CRH) in the brain and pituitary of the hagfish, Eptatretus burgeri. Gen Comp Endocrinol 236:174-180. doi: 10.1016/j.ygcen.2016.07.018 PMID: 27444128
Summary: The distribution of corticotropin-releasing hormone (CRH) in the brain and pituitary of the hagfish Eptatretus burgeri, representing the earliest branch of vertebrates, was examined by immunohistochemistry to better understand the neuroendocrine system of hagfish. A rabbit polyclonal antibody raised against human/mouse/rat CRH (Cat. #AB-02) was used. A standard curve was obtained from 0.78 ng/ml to 50 ng/ml. The cross-reactivity of anti-CRH antibody against CRH family peptides was found to be less than 0.01%, indicating the specificity of the antibody. The specificity of the antibody raised against human/mouse/rat CRH was demonstrated by a TR-FIA and absorption test. CRH-ir cell bodies were detected in two brain regions; the preopticohypothalamic area (PO, POp, and Hyinf) and the medulla oblongata. CRH-ir fibers were mainly distributed in the hypothalamus and the medulla oblongata, in which CRH-ir cell bodies were detected.
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Corticotropin-releasing factor critical for zebrafish camouflage behavior is regulated by light and sensitive to ethanol.
Wagle M, Mathur P, Guo S (2011) Corticotropin-releasing factor critical for zebrafish camouflage behavior is regulated by light and sensitive to ethanol. J Neurosci 31(1):214-224. doi: 10.1523/JNEUROSCI.3339-10.2011 PMID: 21209207
Objective: To explore neural circuit assembly and function using the hardwired camouflage response of zebrafish.
Summary: Both light exposure and ethanol affect the camouflage response. Understanding this system could provide a tool to further investigate the effect of alcohol on neural circuits.
Usage: Immunostaining (1:500)
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
Oxytocin deficiency mediates hyperphagic obesity of Sim1 haploinsufficient mice.
Kublaoui BM, Gemelli T, Tolson KP, Wang Y, Zinn AR (2008) Oxytocin deficiency mediates hyperphagic obesity of Sim1 haploinsufficient mice. Mol Endocrinol 22(7):1723-1734. doi: 10.1210/me.2008-0067 PMID: 18451093
Summary: Central administration of neuropeptides in the paraventricular nucleus (PVN) is known to inhibit feeding. Here the authors examined altered hypothalamic expression of PVN neuropeptides in Sim1+/- mice. Hypothalamic expression of several neuropeptides, including corticotrophin releasing hormone (Crh) was measured. To do so, anti-Crh (Cat. #AB-02, 1:800) was used in immunohistochemistry. The data suggests that these neurons are involved in melanocortin feeding circuits.
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
Expression and role of the corticotropin-releasing hormone/urocortin-receptor-binding protein system in the primate corpus luteum during the menstrual cycle.
Xu J, Xu F, Hennebold JD, Molskness TA, Stouffer RL (2007) Expression and role of the corticotropin-releasing hormone/urocortin-receptor-binding protein system in the primate corpus luteum during the menstrual cycle. Endocrinology 148(11):5385-5395. doi: 10.1210/en.2007-0541 PMID: 17690168
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
Corticotropin-releasing hormone (CRH) requirement in Clostridium difficile toxin A-mediated intestinal inflammation.
Anton PM, Gay J, Mykoniatis A, Pan A, O’Brien M, Brown D, Karalis K, Pothoulakis C (2004) Corticotropin-releasing hormone (CRH) requirement in Clostridium difficile toxin A-mediated intestinal inflammation. Proc Natl Acad Sci U S A 101(22):8503-8508. doi: 10.1073/pnas.0402693101 PMID: 15159534
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
Secretion of corticotropin-releasing factor from cultured rat hypothalamic cells: effects of catecholamines.
Widmaier EP, Lim AT, Vale W (1989) Secretion of corticotropin-releasing factor from cultured rat hypothalamic cells: effects of catecholamines. Endocrinology 124:583-590. doi: 10.1210/endo-124-2-583 PMID: 2783568
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
Topographical and ontogenetic study of the neurons producing growth hormone-releasing factor in human hypothalamus.
Bloch B, Gaillard RC, Brazeau P, Lin HD, Ling N (1984) Topographical and ontogenetic study of the neurons producing growth hormone-releasing factor in human hypothalamus. Regul Pept 8(1):21-31. doi: 10.1016/0167-0115(84)90025-9 PMID: 6425993
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Assay of corticotropin releasing factor.
Vale W, Vaughan J, Yamamoto G, Bruhn T, Douglas C, Dalton D, Rivier C, Rivier J (1983) Assay of corticotropin releasing factor. In: Methods in Enzymology 103:565-577. Elsevier. doi: 10.1016/s0076-6879(83)03040-2 PMID: 6321896
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)