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Toll-like receptor 4-mediated enteric glia loss is critical for the development of necrotizing enterocolitis
Kovler ML, Gonzalez Salazar AJ, Fulton WB, Lu P, Yamaguchi Y, Zhou Q, Sampah M, Ishiyama A, Prindle T Jr, Wang S, Jia H, Wipf P, Sodhi CP, Hackam DJ (2021) Toll-like receptor 4-mediated enteric glia loss is critical for the development of necrotizing enterocolitis. Sci Transl Med 13(612):eabg3459. doi: 10.1126/scitranslmed.abg3459 PMID: 34550727
Objective: Hypothesized that enteric glia loss in the premature intestine could lead to dysmotility, exaggerated Toll-like receptor 4 (TLR4) signaling and necrotizing enterocolitis (NEC) development.
Summary: TLR4-induced enteric glia loss leads to experimental NEC through Brain Derived Neurotrophic Factor (BDNF) loss, which can be treated with compound ‘J11.’
Usage: Immunofluorescence
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Dorso-ventral heterogeneity in tracheal basal stem cells
Tadokoro T, Tanaka K, Osakabe S, Kato M, Kobayashi H, Hogan BLM, Taniguchi H (2021) Dorso-ventral heterogeneity in tracheal basal stem cells. Biol Open 10(9):bio058676. doi: 10.1242/bio.058676 PMID: 34396394
Objective: To determine whether tracheal basal cells (BCs) from the dorso-ventral airways have intrinsic molecular and behavioural differences relevant to their in vivo function.
Summary: This study revealed the differences in the characteristics of BCs based on spatial distribution, and also indicated the role of epithelial-mesenchymal interactions in tissue homoeostasis in the trachea. The findings of this study suggest that careful observation is necessary in future research, as the influence of environment on the phenomenon should be considered.
Usage: Immunohistochemistry (1:100)
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Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfβ-Id2 axis, for tissue regeneration
Kiyokawa H, Yamaoka A, Matsuoka C, Tokuhara T, Abe T, Morimoto M (2021) Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfβ-Id2 axis, for tissue regeneration. Dev Cell 56(13):1917-1929.e9. doi: 10.1016/j.devcel.2021.05.016 PMID: 34129836
Objective: To investigate the molecular mechanisms regulating how basal cells contribute to adult tissue regeneration by shifting from slow cycling to proliferating and subsequently back to slow cycling.
Summary: TGF-β-Id2 axis commonly regulates the proliferation transitions in basal cells during development and regeneration, and its fine-tuning is critical for normal regeneration while avoiding basal cell hyperplasia.
Usage: Immunostaining
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)
Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine
Yuan H, Hu H, Chen R, Mu W, Wang L, Li Y, Chen Y, Ding X, Xi Y, Mao S, Jiang M, Chen J, He Y, Wang L, Dong Y, Tou J, Chen W (2021) Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine. Stem Cells Transl Med 10(6):922-938. doi: 10.1002/sctm.20-0469 PMID: 33481357
Objective: To determine whether premigratory NCSCs (pNCSCs) could form enteric neurons and mediate the motility.
Summary: The results show that the pNCSCs that were previously assumed to not be induced by intestinal environment or cues can innervate the intestine and establish neuron-dependent motility.
Usage: Immunochemistry (1:200)
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)
Peripheral nerve regeneration with 3D printed bionic scaffolds loading neural crest stem cell derived Schwann cell progenitors
Li Y, Lv S, Yuan H, Ye G, Mu W, Fu Y, Zhang X, Feng Z, He Y, Chen W (2021) Peripheral nerve regeneration with 3D printed bionic scaffolds loading neural crest stem cell derived Schwann cell progenitors. Advanced Functional Materials 31(16):2010215. doi: 10.1002/adfm.202010215
Objective: Regeneration efficiency and behavior of peripheral nerves are compared under three treatment strategies: 1) transplantation of Schwann cell progenitors induced from purified neural crest stem cells; 2) implantation of a multiscale scaffold based on high-resolution 3D printing; and 3) implantation of this bionic scaffold loading Schwann cell progenitors.
Summary: Implantation of multiscale scaffolds preloaded with neural crest stem cell derived Schwann cell progenitors is the best strategy to promote peripheral nerve regeneration with improved anatomy and function among the three different strategies.
Usage: Immunostaining (1:200)
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)
Sox10 functions as an inducer of the direct conversion of keratinocytes into neural crest cells
Motohashi T, Kawamura N, Watanabe N, Kitagawa D, Goshima N, Kunisada T (2020) Sox10 functions as an inducer of the direct conversion of keratinocytes into neural crest cells. Stem Cells Dev 29(23):1510-1519. doi: 10.1089/scd.2020.0106 PMID: 33040687
Objective: To investigate whether Sox10 functions in the direct conversion of other somatic cells into neural crest cells (NCCs).
Summary: Results demonstrate that Sox10 functions as an inducer of direct conversion into NCCs in cooperation with the transcription factors involved in NCC generation.
Usage: Flow cytometry and cell sorting
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Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung
Cassandras M, Wang C, Kathiriya J, Tsukui T, Matatia P, Matthay M, Wolters P, Molofsky A, Sheppard D, Chapman H, Peng T (2020) Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung. Nat Cell Biol 22(11):1295-1306. doi: 10.1038/s41556-020-00591-9 PMID: 33046884
Objective: To show that Gli1+ mesenchymal stromal cells (MSCs) promote metaplastic differentiation of airway progenitors into KRT5+ basal cells.
Summary: Gli1+ MSCs integrate hedgehog signaling as a rheostat to control BMP activation in the progenitor niche to determine regenerative outcome in fibrosis.
Usage: Histology and Immunofluorescence (1:200)
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Cell lineage tracing identifies hormone-regulated and wnt-responsive vaginal epithelial stem cells
Ali A, Syed SM, Jamaluddin MFB, Colino-Sanguino Y, Gallego-Ortega D, Tanwar PS (2020) Cell lineage tracing identifies hormone-regulated and wnt-responsive vaginal epithelial stem cells. Cell Rep 30(5):1463-1477.e7. doi: 10.1016/j.celrep.2020.01.003 PMID: 32023462
Objective: To learn more about the role of Wnt signaling in vaginal epithelium (VE) homeostasis.
Summary: Data define heterogeneity in VE and identify VE stem cells. It was shown that canonical Wnt/b-catenin signaling is required for the proliferation and differentiation of VE stem cells.
Usage: histology and immunostaining (1:250)
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Development of intestinal scaffolds that mimic native mammalian intestinal tissue
Ladd MR, Costello CM, Gosztyla C, Werts AD, Johnson B, Fulton WB, Martin LY, Redfield EJ, Crawford B, Panaparambil R, Sodhi CP, March JC, Hackam DJ (2019) Development of intestinal scaffolds that mimic native mammalian intestinal tissue. Tissue Eng Part A 25(17-18):1225-1241. doi: 10.1089/ten.TEA.2018.0239 PMID: 30652526
Objective: To develop a scaffold for the generation of an artificial intestine that specifically mimics the architecture and biomechanical properties of the native small intestine, and to evaluate the scaffold in vitro and in vivo.
Summary: Novel scaffolds were developed using PGS with promise for use in artificial intestine for individuals with short bowel syndrome.
Usage: Histology, immunohistochemistry, microscopy (1:500)
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Nicotinamide mononucleotide adenylyltransferase 2 maintains neuronal structural integrity through the maintenance of golgi structure.
Pottorf T, Mann A, Fross S, Mansel C, Vohra BPS (2018) Nicotinamide mononucleotide adenylyltransferase 2 maintains neuronal structural integrity through the maintenance of golgi structure. Neurochem Int 121:86-97. doi: 10.1016/j.neuint.2018.09.010 PMID: 30278188
Objective: To Investigate the link between NMNAT2 loss, Golgi fragmentation and axon degeneration
Summary: Results degeneration signals converge on a common cytNmnat1 mediated axon protective program and are distinct from the SARM1 mediated caspase independent axon degeneration
Usage: Immunohistochemistry (1:1000)
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)