Anacker A, Esser KH, Lenarz T, Paasche G (2022) Purification of fibroblasts from the spiral ganglion. Front Neurol 13:877342. doi: 10.3389/fneur.2022.877342 PMID: 35493807

Objective: To provide a purified preparation of cochlear fibroblasts, which can be used in vitro for further investigations.

Summary: Subcultivation of the primary spiral ganglion cells culture removed the neurons from the culture and increased the fibroblast to glial cell ratio in the preparations. Direct immunolabeling for the Thy1-glycoprotein and the p75NGFR-enabled fluorescence-based cell sorting. This resulted in a cell culture of cochlear fibroblasts with a purity of more than 99%.

Usage: Cell Sorting (1:400)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Serapiglia V, Stephens CA, Joshi R, Aydin E, Oria M, Marotta M, Peiro JL, Varisco BM (2022) Fetal tracheal occlusion increases lung basal cells via increased yap signaling. Front Pediatr 9:780166. doi: 10.3389/fped.2021.780166 PMID: 35280447

Objective: To test if lung epithelial cell populations alter after tracheal occlusion (TO) with transcriptomic signatures implicating basal cells.

Summary: TO increases lung epithelial cell nuclear Yap, leading to basal cell expansion.

Usage: Immunohistochemistry (1:1000)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Napoli M, Wu SJ, Gore BL, Abbas HA, Lee K, Checker R, Dhar S, Rajapakshe K, Tan AC, Lee MG, Coarfa C, Flores ER (2022) ΔNp63 regulates a common landscape of enhancer associated genes in non-small cell lung cancer. Nat Commun 13(1):614. doi: 10.1038/s41467-022-28202-1 PMID: 35105868

Objective: To investigate the underlying mechanistic role regulated by ΔNp63 in lung cancer development.

Summary: Use of a ΔNp63-specific conditional knockout mouse model and xenograft models of lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC). Results show that ΔNp63 promotes non-small cell lung cancer by maintaining the lung stem cells necessary for lung cancer cell initiation and progression in quiescence. ΔNp63 regulates enhancers of cell identity genes.

Usage: Immunofluorescence and IHC (1:100)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Schulze J, Staecker H, Wedekind D, Lenarz T, Warnecke A (2022) Expression pattern of brain-derived neurotrophic factor and its associated receptors: Implications for exogenous neurotrophin application. Hear Res 413:108098. doi: 10.1016/j.heares.2020.108098 PMID: 33143996

Objective: To investigate the mechanisms of brain-derived neurotrophic factor (BDNF) in the inner ear, by analyzing the expression of mature BDNF (mBDNF), its proform proBDNF and their respective receptors the low affinity p75 neurotrophin receptor (p75NTR) and the neurotrophic receptor tyrosine kinase 2 (NTRK2).

Summary: Treatment with proBDNF is not toxic for spiral ganglion neuron (SGN) survival but simultaneously not protective. However, combined treatment of mBDNF and proBDNF maintained and perhaps slightly increased the protective effect of mBDNF.

Usage: Immunohistochemistry (1:250); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Joshi R, Batie MR, Fan Q, Varisco BM (2022) Mouse lung organoid responses to reduced, increased, and cyclic stretch. Am J Physiol Lung Cell Mol Physiol 322(1):L162-L173. doi: 10.1152/ajplung.00310.2020 PMID: 34851724

Objective: To test two mouse lung organoid mechanotransductive models to address deficiencies in cell culture models.

Summary: Cyclic stretch increased TGF-β and integrin-mediated signaling, with upstream analysis indicating roles for histone deacetylases, microRNAs, and long noncoding RNAs.

Usage: Immunostaining

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Li Y, Hollis E (2021) Basal forebrain cholinergic neurons selectively drive coordinated motor learning in mice. J Neurosci 41(49):10148-10160. doi: 10.1523/JNEUROSCI.1152-21.2021 PMID: 34750228

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP), mu p75-SAP (Cat. #IT-16)

Kovler ML, Gonzalez Salazar AJ, Fulton WB, Lu P, Yamaguchi Y, Zhou Q, Sampah M, Ishiyama A, Prindle T Jr, Wang S, Jia H, Wipf P, Sodhi CP, Hackam DJ (2021) Toll-like receptor 4-mediated enteric glia loss is critical for the development of necrotizing enterocolitis. Sci Transl Med 13(612):eabg3459. doi: 10.1126/scitranslmed.abg3459 PMID: 34550727

Objective: Hypothesized that enteric glia loss in the premature intestine could lead to dysmotility, exaggerated Toll-like receptor 4 (TLR4) signaling and necrotizing enterocolitis (NEC) development.

Summary: TLR4-induced enteric glia loss leads to experimental NEC through Brain Derived Neurotrophic Factor (BDNF) loss, which can be treated with compound ‘J11.’

Usage: Immunofluorescence

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Tadokoro T, Tanaka K, Osakabe S, Kato M, Kobayashi H, Hogan BLM, Taniguchi H (2021) Dorso-ventral heterogeneity in tracheal basal stem cells. Biol Open 10(9):bio058676. doi: 10.1242/bio.058676 PMID: 34396394

Objective: To determine whether tracheal basal cells (BCs) from the dorso-ventral airways have intrinsic molecular and behavioural differences relevant to their in vivo function.

Summary: This study revealed the differences in the characteristics of BCs based on spatial distribution, and also indicated the role of epithelial-mesenchymal interactions in tissue homoeostasis in the trachea. The findings of this study suggest that careful observation is necessary in future research, as the influence of environment on the phenomenon should be considered.

Usage: Immunohistochemistry (1:100)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Cui YH, Zhou SF, Liu Y, Wang S, Li F, Dai RP, Hu ZL, Li CQ (2021) Injection of anti-proBDNF attenuates hippocampal-dependent learning and memory dysfunction in mice with sepsis-associated encephalopathy. Front Neurosci 15:665757. doi: 10.3389/fnins.2021.665757 PMID: 34354558

Objective: To study the effects and associated mechanism of hippocampal proBDNF in a lipopolysaccharide (LPS)-induced sepsis-associated encephalopathy (SAE) mouse model.

Summary: Mice showed cognitive dysfunction after LPS injection. The expression of proBDNF and its receptor, p75NTR, was increased in the hippocampus. The levels of BDNF and its receptor, TrkB, were decreased. An intrahippocampal or intraperitoneal injection of anti-proBDNF antibody ameliorated LPS-induced cognitive dysfunction.

Usage: Western blot (1:1000)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Kiyokawa H, Yamaoka A, Matsuoka C, Tokuhara T, Abe T, Morimoto M (2021) Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfβ-Id2 axis, for tissue regeneration. Dev Cell 56(13):1917-1929.e9. doi: 10.1016/j.devcel.2021.05.016 PMID: 34129836

Objective: To investigate the molecular mechanisms regulating how basal cells contribute to adult tissue regeneration by shifting from slow cycling to proliferating and subsequently back to slow cycling.

Summary: TGF-β-Id2 axis commonly regulates the proliferation transitions in basal cells during development and regeneration, and its fine-tuning is critical for normal regeneration while avoiding basal cell hyperplasia.

Usage: Immunostaining

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)