Collins BE, Blixt O, Han S, Duong B, Li H, Nathan JK, Bovin N, Paulson JC (2006) High-affinity ligand probes of CD22 overcome the threshold set by cis ligands to allow for binding, endocytosis, and killing of B cells. J Immunol 177(5):2994-3003. doi: 10.4049/jimmunol.177.5.2994
Objective: To demonstrate the dynamic equilibrium that exists between CD22 (Siglec-2) and its cis and trans ligands, using a high-affinity multivalent sialoside probe that competes with cis ligands and binds to CD22 on native human and murine B cells.
Summary: The CD22 (Siglec-2) preferred ligand: sequence Siaa2-6Gal that is abundantly expressed on N-linked glycans of B cell glycoproteins. Conjugation of the sialoside probes to the toxin saporin resulted in toxin uptake and toxin-mediated killing of B lymphoma cell lines, suggesting an alternative approach for targeting CD22 for treatment of B cell lymphomas.
Usage: Cytotoxicity assay: BJAB lymphoma cell killing required both the targeting probe and the Streptavidin-ZAP, as no killing was observed in the absence of either.
Related Products: Streptavidin-ZAP (Cat. #IT-27)