Gerashchenko D, Chou TC, Blanco-Centurion CA, Saper CB, Shiromani PJ (2004) Effects of lesions of the histaminergic tuberomammillary nucleus on spontaneous sleep in rats. Sleep 27(7):1275-1281. doi: 10.1093/sleep/27.7.1275
Summary: Although evidence suggests that histaminergic neurons in the tuberomammillary nucleus (TMN) promote wakefulness, this has not been investigated using specific lesioning agents. In this study, the authors utilize the fact that TMN neurons express the orexin-B receptor by eliminating these neurons with an injection of 50 ng of orexin-SAP (Cat. #IT-20) into the posterior hypothalamus. The data indicate that histaminergic neurons are not required for the homeostatic regulation of sleep.
Related Products: Orexin-B-SAP (Cat. #IT-20)